55 research outputs found
Evaluation of microbial and enzymatic communities in soil and rizosphere from soybean plants.
Understanding the biological and biochemical soil properties, as well
as its enzymatic activity is important in designing an efficient
alternative to demonstrate desired modifications in the soil. Such
modifications are related to crop systems, cultivation practices or
other human activities. The objective of this study was to evaluate the
diversity of some microorganisms ( Bacillus , Pseudomonas ,
Trichoderma and Fusarium ) and enzymatic activity in soil from
soybean crops with different yields. Soil sampling was determined
according to the productivity yields of the property, which were
divided into high, medium, and low yield. Microorganisms were evaluated
by counting populations of fungi and bacteria through serial dilutions
of total bacteria, Bacillus, Pseudomonas, Trichoderma and Fusarium. The
\u3b2-glucosidase, acid phosphatase and arylsulphatase activities were
determined by spectrophotometry after one hour of incubation at 37
\ub0C, with the specific substrate
p-nitrophenol-\u3b2-D-glucopyranoside, p-nitrophenol-phosphate and
p-nitrophenyl sulphate, respectively, for each studied enzyme. Urease
was determined by the ammonium release method, after incubating the
soil with urea for two hours, at 37 \ub0C. Soil samples from the
high-yield plot had higher concentrations of Bacillus and total
bacteria. The low-yield soil showed a higher level of colonies of the
genus Fusarium. The studied enzyme activities (\u3b2-glycosidase,
arylsulphatase and urea) were found at lower values in soil samples
from the high-yield field and differed statistically from the low-yield
field. However, in the rhizosphere samples, these enzymes had a higher
activity in the high-yield field. In view of these results, it is
possible that the yield of soybean plants influences the number of
microorganisms and the enzymatic activity of the soil microbiota.Comprendre les propri\ue9t\ue9s biologiques et biochimiques du sol,
de m\ueame que son activit\ue9 enzymatique est important dans la
mise \u153uvre d\u2019une alternative efficiente pour d\ue9montrer
les modifications d\ue9sir\ue9es dans le sol. De telles
modifications sont reli\ue9es aux syst\ue8mes de culture, pratiques
culturales ou les autres activit\ue9s humaines. L\u2019objectif de
cette \ue9tude \ue9tait d\u2019\ue9valuer la diversit\ue9 de
quelques microorganismes ( Bacillus , Pseudomonas , Trichoderma
and Fusarium ) et activit\ue9 enzymatique dans le sol des cultures
du soja avec diff\ue9rents rendements. L\u2019\ue9chantillonnage
des sols \ue9tait d\ue9termin\ue9 selon la productivit\ue9 de
la propri\ue9t\ue9, qui \ue9tait divis\ue9e en
\ue9lev\ue9e, moyenne et faible rendement. Les microorganismes
\ue9taient \ue9valu\ue9s par comptage des populations des
champignons et bact\ue9ries \ue0 travers des s\ue9ries de
dilutions des bact\ue9ries totales, Bacillus, Pseudomonas,
Trichoderma and Fusarium. Les activit\ue9s du b-glucosidase, acide
phosphatase et arylsulphatase \ue9taient d\ue9termin\ue9es par la
spectrophotom\ue9trie apr\ue8s une heure d\u2019incubation \ue0
37\ub0C, avec le substrat sp\ue9cifique de
p-nitrophenol-\u3b2-D-glucopyranoside, p-nitrophenol-phosphate et
p-nitrophenyl sulphate, respectivement, pour chaque enzyme
\ue9tudi\ue9e. Ur\ue9ase \ue9tait d\ue9termin\ue9e par la
m\ue9thode de la lib\ue9ration d\u2019ammonium, apr\ue8s
incubation du sol avec l\u2019ur\ue9e pendant deux heures, \ue0
37\ub0C. Les \ue9chantillons des terres \ue0 haut rendement ont
les concentrations \ue9lev\ue9es en Bacillus et bact\ue9rie
totale. Les terres \ue0 faible rendement ont montr\ue9 un niveau
\ue9lev\ue9 de colonies du genre Fusarium. Les activit\ue9s des
enzymes \ue9tudi\ue9es (b-glycosidase, arylsulphatase et ur\ue9e)
\ue9taient trouv\ue9es \ue0 de faibles valeurs dans les
\ue9chantillons de terres \ue0 haut rendement et diff\ue8rent
statistiquement des terres \ue0 faible rendement. N\ue9anmoins,
dans les \ue9chantillons de la rhizosph\ue8re, ces enzymes ont une
activit\ue9 \ue9lev\ue9e sur les terres \ue0 rendement
\ue9lev\ue9. D\u2019apr\ue8s ces r\ue9sultats, il est possible
que le rendement des plants du soja influence le nombre de
microorganismes et l\u2019activit\ue9 des enzymes des sols
microbiote
Contagem bacteriana total do leite em diferentes sistemas de ordenha e de resfriamento
Tamanho da parcela para estudos de recuperação de fertilizante-15N por capim-tanzùnia
Closed soilless growing system for producing strawberry bare root transplants and runner tips
Semeadura do milho em sistema de plantio direto sem e com manejo mecùnico da matéria seca
Tracking viral particles in the intestinal contents of the American bullfrog, Lithobates catesbeianus, by Transmission Electron Microscopy
Atributos fĂsicos, quĂmicos e biolĂłgicos de um Latossolo de cerrado em plantio de espĂ©cies florestais
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