Human Serum Folate Can Be Measured Using Folate Binding Protein Linked To Enzyme-labeled Protein Ligand Binding Assay (Elplba) AS Well AS Elisa

Background: Folate is an important substance used for purine and pyrimidine nucleotide synthesis. One measurement of folate that already establishes is using ELISA (Enzyme-linked immunosorbent assay) method. Folate binding protein is a protein that can bind folate, therefore it considered can be used as a tool that can replace antibody dependent ELISA method.Objectives: The aim of this research was to create a method for folate measurement in serum called Enzyme-labeled protein ligand binding assay (ELPLBA) by replacing antibody as used in ELISA method with folate binding protein (FBP) that purified from the whey of milk.Methods: The method is tested using 20 serum samples and compared to ELISA. Folate binding protein was purified from bovine\u27s milk using ammonium sulfate up to 90% saturated, DEAE-cellulose anion exchange chromatography and affinity chromatography. SDS-PAGE and western blot were used to establish the protein band of FBP that has molecular weight of ~25-35 kDa. ELPLBA was arranged with stationary phase using aminohexyl-agarose, and folic acid linked on it using carbodiimide.Results: The result show there was no significant difference of folate concentration between ELPLBA (14.804 ± 2.795) and ELISA method (13.859 ± 3.638), p = 0.363.Conclusion: ELPLBA method show similarity for determination of folate in serum which was the same as standard folate measurement (ELISA)

Perencanaan Jaringan Komunikasi Berbasis Voip Menggunakan Cisco Unified Communication Manager

Computer network technology is growing rapidly. Computer communication technology is very Fast developing and has developed application systems such as VoIP (Voice Over Internet Protocol). This technology works by converting sound into a digital format that is sent over the internet. Prior to the current implementation, the voice or telephone communication network used at the head office of PT. SequisLife uses a PSTN-based telephone. This, of course, wastes the company's operational financing. By using VoIP (Voice Over Internet Protocol) to create a very appropriate solution to resolve what happened to the company. Building a VoIP server requires an open source based application, namely the CUCM application, with sufficient CUCM applications requiring functions and benefits that are stable and safe to use. To build a VoIP-based communication network only requires a separate server, ip phone, router and internet network connection. With the results obtained by communication planning with VoIP-based technology, employees or users associated with the environment of this company in one another cooperation safely and smoothly and low cos