Asthma: a comparison of animal models using stereological methods

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Efflux of Naphthalene Oxide and Reactive Naphthalene Metabolites from Isolated Hepatocytes1

by Renton et at. (1978) and Klaunig et at. (1981). Briefly, the mouse was treated i.v. 30 mm before the start of surgery with 100 U of heparin. The animal was anesthetized with ether, the abdomen and chest cavity opened and a cannula inserted Vol. 242 into the vena cava through the right atrium. To determine quantitatively the rate oftransfer of naphthalene 1,2-oxide across intact hepatocyte membranes, a method had to be developed that was capable of trapping naphthalene oxide on the exterior of the cell. The approach used in the current studies is summarized i