Effect of genotype on duodenal expression of nutrient transporter genes in dairy cows

peer-reviewedBackground Studies have shown clear differences between dairy breeds in their feed intake and production efficiencies. The duodenum is critical in the coordination of digestion and absorption of nutrients. This study examined gene transcript abundance of important classes of nutrient transporters in the duodenum of non lactating dairy cows of different feed efficiency potential, namely Holstein-Friesian (HF), Jersey (JE) and their F1 hybrid. Duodenal epithelial tissue was collected at slaughter and stored at -80°C. Total RNA was extracted from tissue and reverse transcribed to generate cDNA. Gene expression of the following transporters, namely nucleoside; amino acid; sugar; mineral; and lipid transporters was measured using quantitative real-time RT-PCR. Data were statistically analysed using mixed models ANOVA in SAS. Orthogonal contrasts were used to test for potential heterotic effects and spearman correlation coefficients calculated to determine potential associations amongst gene expression values and production efficiency variables. Results While there were no direct effects of genotype on expression values for any of the genes examined, there was evidence for a heterotic effect (P < 0.05) on ABCG8, in the form of increased expression in the F1 genotype compared to either of the two parent breeds. Additionally, a tendency for increased expression of the amino acid transporters, SLC3A1 (P = 0.072), SLC3A2 (P = 0.081) and SLC6A14 (P = 0.072) was also evident in the F1 genotype. A negative (P < 0.05) association was identified between the expression of the glucose transporter gene SLC5A1 and total lactational milk solids yield, corrected for body weight. Positive correlations (P < 0.05) were also observed between the expression values of genes involved in common transporter roles. Conclusion This study suggests that differences in the expression of sterol and amino acid transporters in the duodenum could contribute towards the documented differences in feed efficiency between HF, JE and their F1 hybrid. Furthermore, positive associations between the expression of genes involved in common transporter roles suggest that these may be co-regulated. The study identifies potential candidates for investigation of genetic variants regulating nutrient transport and absorption in the duodenum in dairy cows, which may be incorporated into future breeding programmes

Observing CVs and LMXBs with SALT: Updates and Recent Results

I present an overview of the ongoing observational programs utilizing the Southern African Large Telescope (SALT), focussing on magnetic Cataclysmic Variables and Low Mass X-ray Binaries. SALT’s instruments and capabilities are well suited to time resolved studies of the accretion phenomena exhibited in these systems. Initial observations, using SALTICAM, have been used to derive high time resolution (~100ms) eclipse light curves, with high signal-to-noise, of Polars. Recently this work has been extended to time resolved spectroscopic studies, utilizing the SALT Robert Stobie Spectrograph (RSS), allowing an opportunity to probe how the emission lines change during eclipse. A program to search for and characterize quasi-periodic oscillations (QPOs) in magnetic CVs using the SAAO 1.9-m telescope, begun in 2012, has been expanded to include observations with a photon counting camera (BVIT) on SALT. A multi-longitude campaign involving SALT, other SAAO facilities plus the ESO NTT, was carried out in March/April 2012 on the enigmatic gamma ray source, XSS J12270–4859, which has revealed the that system exhibits spectral line variations, from absorption to emission, seemingly over timescales of &lt; 1 h

Adding value to cull cow beef

End of project reportThis project addressed the prospects of increasing the value of cull cow beef and examined the potential of a number of different management and dietary strategies. In Ireland, the national cow herd contributes 350,000 animals to total beef production annually, which represents 22% of all cattle slaughtered (DAF, 2007). A dominant feature of beef production in Ireland is the disposal of cows from the dairy and beef industries, the time of year at which culling occurs influences the number of cows available for slaughter. Suitability of a cow for slaughter is generally not a consideration for dairy or beef farmers

Prediction of cull cow carcass characteristics from live weight and body condition score measured pre slaughter

peer-reviewedA study was conducted to provide information on the degree of carcass finish of Irish cull cows and to investigate the usefulness of live animal measurements for the prediction beef breeds (albeit with a moderate R2 value compared to the carcass weight prediction) using objective, non-intrusive and easily measured live animal measurements, should be of benefit to farmers finishing cull cows in Ireland. of cull cow carcass characteristics. Live weight (LW) and body condition score (BCS) were recorded on cows entering an Irish commercial slaughter facility between September and November, 2005. Data pertaining to sire breed, age and carcass characteristics were collected and subsequently collated for each cow. For analysis, cows (n = 2163) were subdivided into three breed categories: dairy breed sired by Holstein/ Friesian (FR), sired by early-maturing beef breeds (EM) and sired by late-maturing beef breeds (LM). The proportion of cows slaughtered at the desired (TARGET) carcass standard (cold carcass weight ≥ 272 kg, carcass conformation class ≥ P+ and carcass fat class ≥ 3) was low (on average 0.30), but did differ (P < 0.001) between the dairy and beef breed categories (0.22, 0.47 and 0.53 for FR, EM and LM categories, respectively). Regression procedures were used to develop equations to predict cold carcass weight, carcass conformation score, carcass fat score and proportion in the TARGET category from LW and BCS. Equations predicting cold carcass weight had high R2 values for all breed categories (0.81, 0.85 and 0.79 for the FR, EM and LM, respectively). Equations predicting carcass fatness had moderate R2 values for the beef breed categories (0.65 and 0.59 for the EM and LM, respectively). Equations predicting carcass conformation and the TARGET category yielded lower R2 values. The successful prediction of carcass weight for all breed categories and of carcass fatness for th

Adhesion, friction, and deformation of ion-beam-deposited boron nitride films

The tribological properties and mechanical strength of boron nitride films were investigated. The BN films were predominantly amorphous and nonstoichiometric and contained small amounts of oxides and carbides. It was found that the yield pressure at full plasticity, the critical load to fracture, and the shear strength of interfacial adhesive bonds (considered as adhesion) depended on the type of metallic substrate on which the BN was deposited. The harder the substrate, the greater the critical load and the adhesion. The yield pressures of the BN film were 12 GPa for the 440C stainless steel substrate, 4.1 GPa for the 304 stainless steel substrate, and 3.3 GPa for the titanium substrate

SALT Spectropolarimetry and Self-Consistent SED and Polarization Modeling of Blazars

We report on recent results from a target-of-opportunity program to obtain spectropolarimetry observations with the Southern African Large Telescope (SALT) on flaring gamma-ray blazars. SALT spectropolarimetry and contemporaneous multi-wavelength spectral energy distribution (SED) data are being modelled self-consistently with a leptonic single-zone model. Such modeling provides an accurate estimate of the degree of order of the magnetic field in the emission region and the thermal contributions (from the host galaxy and the accretion disk) to the SED, thus putting strong constraints on the physical parameters of the gamma-ray emitting region. For the specific case of the $\gamma$-ray blazar 4C+01.02, we demonstrate that the combined SED and spectropolarimetry modeling constrains the mass of the central black hole in this blazar to $M_{\rm BH} \sim 10^9 \, M_{\odot}$.Comment: Submitted to Galaxies - Proceedings of "Polarized Emission from Astrophysical Jets", Ierapetra, Crete, June 12 - 16, 201