5,274 research outputs found
Characterization of Knots and Links Arising From Site-specific Recombination on Twist Knots
We develop a model characterizing all possible knots and links arising from
recombination starting with a twist knot substrate, extending previous work of
Buck and Flapan. We show that all knot or link products fall into three
well-understood families of knots and links, and prove that given a positive
integer , the number of product knots and links with minimal crossing number
equal to grows proportionally to . In the (common) case of twist knot
substrates whose products have minimal crossing number one more than the
substrate, we prove that the types of products are tightly prescribed. Finally,
we give two simple examples to illustrate how this model can help determine
previously uncharacterized experimental data.Comment: 32 pages, 7 tables, 27 figures, revised: figures re-arranged, and
minor corrections. To appear in Journal of Physics
Single-step method for β-galactosidase assays in Escherichia coli using a 96-well microplate reader
AbstractHistorically, the lacZ gene is one of the most universally used reporters of gene expression in molecular biology. Its activity can be quantified using an artificial substrate, o-nitrophenyl-ß-d-galactopyranoside (ONPG). However, the traditional method for measuring LacZ activity (first described by J. H. Miller in 1972) can be challenging for a large number of samples, is prone to variability, and involves hazardous compounds for lysis (e.g., chloroform, toluene).Here we describe a single-step assay using a 96-well microplate reader with a proven alternative cell permeabilization method. This modified protocol reduces handling time by 90%
Topographic controls on dike injection in volcanic rift zones
Author Posting. © Elsevier B.V., 2006. This is the author's version of the work. It is posted here by permission of Elsevier B.V. for personal use, not for redistribution. The definitive version was published in Earth and Planetary Science Letters 246 (2006): 188-196, doi:10.1016/j.epsl.2006.04.005.Dike emplacement in volcanic rift zones is often associated with the injection of “bladelike”
dikes, which propagate long distances parallel to the rift, but frequently remain
trapped at depth and erupt only near the tip of the dike. Over geologic time, this style of
dike injection implies that a greater percentage of extension is accommodated by magma
accretion at depth than near the surface. In this study, we investigate the evolution of
faulting, topography, and stress state in volcanic rift zones using a kinematic model for
dike injection in an extending 2-D elastic-viscoplastic layer. We show that the intrusion
of blade-like dikes focuses deformation at the rift axis, leading to the formation of an
axial rift valley. However, flexure associated with the development of the rift topography
generates compression at the base of the plate. If the magnitude of these deviatoric
compressive stresses exceeds the deviatoric tensile stress associated with far-field
extension, further dike injection will be inhibited. In general, this transition from tensile
to compressive deviatoric stresses occurs when the rate of accretion in the lower crust is
greater than 50-60% of the far-field extension rate. These results indicate that over
geologic time-scales the injection of blade-like dikes is a self-limiting process in which
dike-generated faulting and topography result in an efficient feedback mechanism that
controls the time-averaged distribution of magma accretion within the crust.Funding
for this research was provided by NSF Grants OCE 04-43246, OCE 05-50147, OCE 02-42597 and OCE 04-26575, and a Carnegie Postdoctoral Fellowship to M.B
A data comparison between a traditional and the single-step β-galactosidase assay
This article describes reproducibility of a single-step automated β-galactosidase, and the equivalence of its data to the traditional assay ("Experiments in Molecular Genetics" [1]). This was done via a pairwise comparison of both methods using strains with Miller Unit [MU] values ranging from 0 to over 2000. The data presented in this article is associated with the research article entitled "A single-step method for mid to high throughput β-galactosidase assays in Escherichia coli using a microplate reader" [2]
Cellular and molecular phenotypes depending upon the RNA repair system RtcAB of Escherichia coli
Biotechnology and Biological Sciences Research Council (BBSRC) [BB/J00717X/1]; Medical Research Council (MRC) [MR/M017672/1]; Queen's Fellowship (Queen's University Belfast, UK) (to C.E.); Antimicrobial Resistance Cross Council Initiative. Funding for open access charge: BBSRC [BB/J00717X/1]; MRC [MR/M017672/1]
Documentation of the data analysis system for the gamma ray monitor aboard OSO-H
The programming system is presented which was developed to prepare the data from the gamma ray monitor on OSO-7 for scientific analysis. The detector, data, and objectives are described in detail. Programs presented include; FEEDER, PASS-1, CAL1, CAL2, PASS-3, Van Allen Belt Predict Program, Computation Center Plot Routine, and Response Function Programs
Information and Particle Physics
Information measures for relativistic quantum spinors are constructed to
satisfy various postulated properties such as normalisation invariance and
positivity. Those measures are then used to motivate generalised Lagrangians
meant to probe shorter distance physics within the maximum uncertainty
framework. The modified evolution equations that follow are necessarily
nonlinear and simultaneously violate Lorentz invariance, supporting previous
heuristic arguments linking quantum nonlinearity with Lorentz violation. The
nonlinear equations also break discrete symmetries. We discuss the implications
of our results for physics in the neutrino sector and cosmology
Bremsstrahlung in Alpha-Decay
We present the first fully quantum mechanical calculation of photon radiation
accompanying charged particle decay from a barrier resonance. The soft-photon
limit agrees with the classical results, but differences appear at
next-to-leading-order. Under the conditions of alpha-decay of heavy nuclei, the
main contribution to the photon emission stems from Coulomb acceleration and
may be computed analytically. We find only a small contribution from the
tunneling wave function under the barrier.Comment: 12 pages, 2 Postscript figure
Possible production of exotic baryonia in relativistic heavy-ion collisions
Properties of a hypothetical baryonium with the quark content
(uds\ov{u}\ov{d}\ov{s}) are discussed. The MIT bag model predicts its mass to
be unexpectedly low, approximately 1210 MeV. Possible hadronic decay modes of
this state are analyzed. Ultrarelativistic heavy-ion collisions provide
favorable conditions for the formation of such particles from the baryon-free
quark-gluon plasma. We estimate multiplicities of such exotic baryonia on the
basis of a simple thermal model.Comment: 8 pages, 1 figur
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