30 years of hay meadow succession without fertilization: how does it affect soil and avifauna groups?

In the present study we investigated the effects of hay meadow succession in the brook valley system of the Drentse A Nature Reserve, in the NE of the Netherlands. In particular, we compared the plant and soil fauna composition in five grasslands that differed in the stage of vegetation succession in two well-studied chronosequences, dry and wet respectively. The sampled meadows include a control site (still fertilized meadow), a 5, 15, 25 and 32 years stage of vegetation succession after the cessation of fertilizer application. At all sites, vegetation and earthworm composition was studied in replicate subplots of 4 m2 respectively 0.04 m² each. Moreover, the breeding birds have been monitored in the area over the last 28 years by mapping territories overlapping the meadows of the chronosequences. Concerning the plants we found that diversity was increasing with time of succession. In the wet meadow series the plant species richness increased from ca. 13 species per site (40 m2) to a maximum of ca. 49 species per site in the latest successional stage. In the drier parts the increase in species was less and reached an over all maximum of 27 species in the intermediate (15 years old) stage of the succession in 40 m2, but still increased to a mean of 15 species at the scale of 4 m2 plots in the oldest stage. The diversity and abundance of earthworms dropped significantly over time of succession. The species all belonged to 4 genera with Allolobophora being the most abundant. Soil pH dropped significantly during the succession even below 3.8 in the dry series. This largely explains the unfavourable conditions for the earthworms to survive in the oldest stages of the dry succession. The earthworm biomass dropped in nearly all sites below 25 g/m2 during the summer period, indicating unfavourable conditions for a suit of grassland breeding waders.The abundance of breeding birds in general was low due to the rather small area covered in this study. Anyhow we could find obvious changes in the breeding bird community. In particular waders such as Lapwing (Vanellus vanellus) and Black-tailed Godwit (Limosa limosa) disappeared completely from the area and were followed up by Curlew (Numenius arquata) and Snipe (Gallinago gallinago) being currently also rare in the area. Probably due to changes in grassland vegetation (increase of amongst others Cirsium palustre) and insect abundance the Stonechat (Saxicola torquata) has entered the area as a breeding bird. The same happens to a set of bird species typical for developing carr woodlands such as Lesser Spotted Woodpecker (Dendrocopos minor) and Golden Oriole (Oriolus oriolus).Die vorliegende Studie thematisiert am Beispiel der Fließgewässerniederung der Drentse A, einem großflächigen Schutzgebiet im Nordosten der Niederlande, die Folgen einer langjährigen Heuwiesennutzung ohne Düngung. Im Einzelnen wurde von uns die Zusammensetzung der Vegetation und Bodenfauna auf fünf Grünlandflächen untersucht, die sich in der Dauer der Ausmagerung unterschieden. Getrennt betrachtet wurden dabei die bachnahen, moorigen Niederungsbereichen und die angrenzenden sandigen Geestbereiche. Die ausgewählten Grünlandflächen waren zum Zeitpunkt der Aufnahmen seit 5, 15, 25, und 32 Jahren gemäht aber nicht mehr gedüngt worden. Eine weitere, nach wie vor konventionell bewirtschaftete Wiesenfläche (incl. Düngung) diente als Kontrolle. Auf allen Flächen wurde die Vegetation und Regenwurmfauna in 10 Plots mit einer Größe von jeweils 4 m² bzw. 0.04 m² untersucht. Darüber hinaus wurden über 28 Jahre hinweg die Brutvögel des Gebietes mittels Revierkartierung erfasst. Die Pflanzenartendiversität hat sich mit Dauer der Ausmagerung signifikant erhöht. Sie stieg in den bachnahen Bereichen von 13 Arten in der Kontrollfläche (40 m²) auf 49 Arten in der am längsten ausgemagerten Grünlandfläche an. In den trockenen Geestbereichen war der Anstieg deutlich schwächer. Bezogen auf die Gesamtfläche von 40 m² wurden die meisten Arten hier in der 15 Jahre lang ausgemagerten Grünlandfläche gefunden, während bei Betrachtung der 4 m² großen Aufnahmepunkte die höchste Artenzahl ebenfalls in der ältesten Untersuchungsfläche lag. Die Diversität und Abundanz der Regenwürmer nahm mit Dauer der Ausmagerung ab. Die festgestellten Arten gehörten zu 4 Gattungen, wobei die Gattung Allolobophora am individuenreichsten vertreten war. Mit Dauer der Ausmagerung sank besonders im trockenen bachfernen Geestbereich der Boden-pH-Wert auf unter 3,8 ab. Die damit einhergehenden pessimalen Lebensbedingungen erklären hinreichend die geringe Diversität und Dichte von Regenwürmern in diesen Bereichen. An fast allen Standorten sank die Biomasse der Regenwürmer zum Sommer hin auf Werte unter 25 g/m², so dass für viele Limikolen zu dieser Zeit pessimale Ernährungsbedingungen bestehen. Die Zahl der Brutvogelarten war aufgrund des recht kleinen Untersuchungsgebietes insgesamt gering. Dennoch konnten auffallende Veränderungen in der Brutvogelgemeinschaft beobachtet werden. Während Limikolen wie Kiebitz (Vanellus vanellus) und Uferschnepfe (Limosa limosa) vollständig aus dem Gebiet verschwanden, wanderten der Große Brachvogel und die Bekassine ein. Allerdings sind auch sie aktuell nur noch selten im Gebiet vertreten. Dafür hat sich inzwischen das Schwarzkehlchen (Saxicola torquata) als Brutvogel eingestellt – möglicherweise eine Folge der sich ändernden Grünlandvegetation (hier: Zunahme von Pflanzenarten, die als Ansitzwarten fungieren können wie etwa Cirsium palustre) in Kombination mit einem verbesserten Nahrungsangebot an Makroinvertebraten. Eingewandert sind zwischenzeitlich auch eine Reihe weiterer Vogelarten, wie Pirol (Oriolus oriolus) und Kleinspecht (Dendrocopos minor), die charakteristisch für sich entwickelnde Bruchwälder sind. Letztere haben sich, meist kleinflächig, auf ehemaligen Feuchtgrünlandstandorten entwickelt

Simple, Efficient Catalyst System for the Palladium-Catalyzed Amination of Aryl Chlorides, Bromides, and Triflates

Palladium complexes supported by (o-biphenyl)P(t-Bu)_2 (3) or (o-biphenyl)PCy_2 (4) are efficient catalysts for the catalytic amination of a wide variety of aryl halides and triflates. Use of ligand 3 allows for the room-temperature catalytic amination of many aryl chloride, bromide, and triflate substrates, while ligand 4 is effective for the amination of functionalized substrates or reactions of acyclic secondary amines. The catalysts perform well for a large number of different substrate combinations at 80−110 °C, including chloropyridines and functionalized aryl halides and triflates using 0.5−1.0 mol % Pd; some reactions proceed efficiently at low catalyst levels (0.05 mol % Pd). These ligands are effective for almost all substrate combinations that have been previously reported with various other ligands, and they represent the most generally effective catalyst system reported to date. Ligands 3 and 4 are air-stable, crystalline solids that are commercially available. Their effectiveness is believed to be due to a combination of steric and electronic properties that promote oxidative addition, Pd−N bond formation, and reductive elimination

Four Poynting Theorems

The Poynting vector is an invaluable tool for analysing electromagnetic problems. However, even a rigorous stress-energy tensor approach can still leave us with the question: is it best defined as \Vec{E} \cross \Vec{H} or as \Vec{D} \cross \Vec{B}? Typical electromagnetic treatments provide yet another perspective: they regard \Vec{E} \cross \Vec{B} as the appropriate definition, because \Vec{E} and \Vec{B} are taken to be the fundamental electromagnetic fields. The astute reader will even notice the fourth possible combination of fields: i.e. \Vec{D} \cross \Vec{H}. Faced with this diverse selection, we have decided to treat each possible flux vector on its merits, deriving its associated energy continuity equation but applying minimal restrictions to the allowed host media. We then discuss each form, and how it represents the response of the medium. Finally, we derive a propagation equation for each flux vector using a directional fields approach; a useful result which enables further interpretation of each flux and its interaction with the medium.Comment: 8 pages. Updated slightly from EJP versio

Absence of a consistent classical equation of motion for a mass-renormalized point charge

The restrictions of analyticity, relativistic (Born) rigidity, and negligible O(a) terms involved in the evaluation of the self electromagnetic force on an extended charged sphere of radius "a" are explicitly revealed and taken into account in order to obtain a classical equation of motion of the extended charge that is both causal and conserves momentum-energy. Because the power-series expansion used in the evaluation of the self force becomes invalid during transition time intervals immediately following the application and termination of an otherwise analytic externally applied force, transition forces must be included during these transition time intervals to remove the noncausal pre-acceleration and pre-deceleration from the solutions to the equation of motion without the transition forces. For the extended charged sphere, the transition forces can be chosen to maintain conservation of momentum-energy in the causal solutions to the equation of motion within the restrictions of relativistic rigidity and negligible O(a) terms under which the equation of motion is derived. However, it is shown that renormalization of the electrostatic mass to a finite value as the radius of the charge approaches zero introduces a violation of momentum-energy conservation into the causal solutions to the equation of motion of the point charge if the magnitude of the external force becomes too large. That is, the causal classical equation of motion of a point charge with renormalized mass experiences a high acceleration catastrophe.Comment: 13 pages, No figure

An efficient system for the Pd-catalyzed cross-coupling of amides and aryl chlorides

A catalyst based on a new biarylphosphine ligand (3) for the Pd-catalyzed cross-coupling reactions of amides and aryl chlorides is described. This system shows the highest turnover frequencies reported to date for these reactions, especially for aryl chloride substrates bearing an ortho substituent. An array of amides and aryl chlorides were successfully reacted in good to excellent yields.National Institutes of Health (U.S.) (Grant GM-58160)Merck & Co., Inc.BASFChemetallNippon Chemical Industrial Co.William Asbornsen Albert Memorial Fund (Fellowship)University of Aarhus. Department of ChemistryUniversity of Aarhus. Interdisciplinary Nanoscience CentreChina Scholarship CouncilNational Natural Science Foundation (China) (Grant 20672088

Search for Magnetic Monopoles Trapped in Matter

There have been many searches for magnetic monopoles in flight, but few for monopoles in matter. We have searched for magnetic monopoles in meteorites, schists, ferromanganese nodules, iron ores and other materials. The detector was a superconducting induction coil connected to a SQUID (Superconducting Quantum Interference Device) with a room temperature bore 15 cm in diameter. We tested a total of more than 331 kg of material including 112 kg of meteorites. We found no monopole and conclude the overall monopole/nucleon ratio in the samples is $<1.2 \times 10^{-29}$ with a 90\% confidence level.Comment: 6 pages, rev tex, no figure

Palladium-catalyzed coupling of functionalized primary and secondary amines with aryl and heteroaryl halides: two ligands suffice in most cases

We report our studies on the use of two catalyst systems, based on the ligands BrettPhos and RuPhos, which provide the widest scope for Pd-catalyzed C–N cross-coupling reactions to date. Often low catalyst loadings and short reaction times can be used with functionalized aryl and heteroaryl coupling partners. The reactions are highly robust and can be set up and performed without the use of a glovebox. These catalysts should find wide application in the synthesis of complex molecules including pharmaceuticals, natural products and functional materials.Amgen Inc.National Institutes of Health (U.S.) (Grant GM-58160)American Chemical SocietyBoehringer IngelheimNational Science Foundation (U.S.) (CHE 9808061)National Science Foundation (U.S.) (DBI 9729592

Stille Cross-Coupling Reactions of Aryl Mesylates and Tosylates Using a Biarylphosphine Based Catalyst System

A catalyst system for the Stille cross-coupling reactions of aryl mesylates and tosylates is reported. Using the combination of Pd(OAc)[subscript 2], XPhos, and CsF in t-BuOH an array of aryl and heteroaryl sulfonates were successfully employed in these reactions. Morever, heteroarylstannanes, such as furyl, thienyl, and N-methylpyrrolyl, which are often prone to decomposition, were efficiently coupled under these conditions. Ortho-substitution on the stannane coupling partner was well tolerated; however, the presence of ortho substituents on the aryl sulfonates greatly reduced the efficiency of these reactions.National Institutes of Health (U.S.) (Grant GM-46059)Merck & Co.Boehringer Ingelheim Pharmaceutical

Virtual screening for inhibitors of the human TSLP:TSLPR interaction

The pro-inflammatory cytokine thymic stromal lymphopoietin (TSLP) plays a pivotal role in the pathophysiology of various allergy disorders that are mediated by type 2 helper T cell (Th2) responses, such as asthma and atopic dermatitis. TSLP forms a ternary complex with the TSLP receptor (TSLPR) and the interleukin-7-receptor subunit alpha (IL-7Ra), thereby activating a signaling cascade that culminates in the release of pro-inflammatory mediators. In this study, we conducted an in silico characterization of the TSLP: TSLPR complex to investigate the drugability of this complex. Two commercially available fragment libraries were screened computationally for possible inhibitors and a selection of fragments was subsequently tested in vitro. The screening setup consisted of two orthogonal assays measuring TSLP binding to TSLPR: a BLI-based assay and a biochemical assay based on a TSLP: alkaline phosphatase fusion protein. Four fragments pertaining to diverse chemical classes were identified to reduce TSLP: TSLPR complex formation to less than 75% in millimolar concentrations. We have used unbiased molecular dynamics simulations to develop a Markov state model that characterized the binding pathway of the most interesting compound. This work provides a proof-ofprinciple for use of fragments in the inhibition of TSLP: TSLPR complexation

Influence of topography on tide propagation and amplification in semi-enclosed basins

An idealized model for tide propagation and amplification in semi-enclosed rectangular basins is presented, accounting for depth differences by a combination of longitudinal and lateral topographic steps. The basin geometry is formed by several adjacent compartments of identical width, each having either a uniform depth or two depths separated by a transverse topographic step. The problem is forced by an incoming Kelvin wave at the open end, while allowing waves to radiate outward. The solution in each compartment is written as the superposition of (semi)-analytical wave solutions in an infinite channel, individually satisfying the depth-averaged linear shallow water equations on the f plane, including bottom friction. A collocation technique is employed to satisfy continuity of elevation and flux across the longitudinal topographic steps between the compartments. The model results show that the tidal wave in shallow parts displays slower propagation, enhanced dissipation and amplified amplitudes. This reveals a resonance mechanism, occurring when\ud the length of the shallow end is roughly an odd multiple of the quarter Kelvin wavelength. Alternatively, for sufficiently wide basins, also Poincaré waves may become resonant. A transverse step implies different wavelengths of the incoming and reflected Kelvin wave, leading to increased amplitudes in shallow regions and a shift of amphidromic points in the direction of the deeper part. Including the shallow parts near the basin’s closed end (thus capturing the Kelvin resonance mechanism) is essential to reproduce semi-diurnal and diurnal\ud tide observations in the Gulf of California, the Adriatic Sea and the Persian Gulf