Expression of <i>HAC1</i> during fed-batch culture, 0 (before), 2 and 4 hours after the start of methanol addition to wild-type GS115 (black), <i>TRY1-1</i>(blue) and <i>TRY1-3</i> (red), strains containing 1 and 3 gene copies of the human typsinogen gene, respectively, under the control of the <i>AOX1</i> promoter.

<p>Error bars show SEM.</p

Expression of the gene encoding D-arabitol-2-dehydrogenase (top) and “<i>YDL124W”</i> (bottom), 0 (before), 2 and 4 hours after the start of methanol addition to wild-type GS115 (black), <i>TRY1-1</i>(blue) and <i>TRY1-3</i> (red), strains containing 1 and 3 gene copies of the human typsinogen gene, respectively, under the control of the <i>AOX1</i> promoter.

<p>Error bars show SEM.</p

Expression of <i>PCK1</i>, <i>PYC</i> and <i>PYK</i> during fed-batch culture, 0 (before), 2 and 4 hours after the start of methanol addition to wild-type GS115 (black), <i>TRY1</i>-1 (blue) and <i>TRY1</i>-3 (red).

<p>Error bars show SEM.</p

Expression of genes associated with methanol oxidation and assimilation during fed-batch culture, 0 (before), 2 and 4 hours after the start of methanol addition to wild-type GS115 (black), <i>TRY1-1</i>(blue) and <i>TRY1-3</i> (red), strains containing 1 and 3 gene copies of the human typsinogen gene, respectively, under the control of the <i>AOX1</i> promoter.

<p>From top to bottom, panels represent <i>AOX1</i>, <i>FDH1</i>, <i>FGH1</i>, <i>DAS1</i>, <i>DAK2</i>, <i>TPI1</i> and <i>RIB1</i>. Error bars show SEM.</p

Metabolite accumulation profiles during fed-batch culture from 0 (before) to 24 hours after the start of methanol addition to wild-type GS115 (black), <i>TRY1-1</i>(blue) and <i>TRY1-3</i> (red), strains containing 1 and 3 gene copies of the human typsinogen gene, respectively, under the control of the <i>AOX1</i> promoter.

<p>From top to bottom, panels represent a) formate (external), b) arabitol (external), c) trehalose (external), d) α / β-D-glucose (external), e) lactate (internal). Error bars show SEM.</p

The number of unique probes on the micro-array per gene.

<p>A total of 3371 genes were targeted by 9 unique probes, with the average length of these genes being 1844 bases.</p><p>The number of unique probes on the micro-array per gene.</p