35 research outputs found
Early-life events may trigger biochemical pathways for Alzheimer’s disease: the “LEARn” model
Dynamic Imaging of LDH Inhibition in Tumors Reveals Rapid in vivo Metabolic Rewiring and Vulnerability to Combination Therapy
Femoral Revision with an Extensively Hydroxyapatite-Coated Femoral Component
Between December 1996 and April 2003, 26 consecutive femoral component revisions in 24 patients were performed with an extensively hydroxyapatite-coated femoral stem. Two patients were lost to follow-up, and two patients died of unrelated causes. Of the 22 femoral revisions in 20 patients, there was a 0% incidence of mechanical loosening at average follow-up of 3.2 years (2–6.3 years). The Harris Hip Score improved from 59 (36 to 83) to 95 (84 to 100) postoperatively (p < 0.001). Rate of revision was 18.2% (4.5% for sepsis, 9.1% for instability, and 4.5% for polyethelene wear). All 22 femoral components had evidence of bone ingrowth. The extensively coated hydroxyapatite stem in this series produced excellent clinical results with a low incidence of thigh pain (4.5%) and severe stress shielding (4.5%)
Discovery and Optimization of Potent, Cell-Active Pyrazole-Based Inhibitors of Lactate Dehydrogenase (LDH)
We report the discovery and medicinal
chemistry optimization of
a novel series of pyrazole-based inhibitors of human lactate dehydrogenase
(LDH). Utilization of a quantitative high-throughput screening paradigm
facilitated hit identification, while structure-based design and multiparameter
optimization enabled the development of compounds with potent enzymatic
and cell-based inhibition of LDH enzymatic activity. Lead compounds
such as <b>63</b> exhibit low nM inhibition of both LDHA and
LDHB, submicromolar inhibition of lactate production, and inhibition
of glycolysis in MiaPaCa2 pancreatic cancer and A673 sarcoma cells.
Moreover, robust target engagement of LDHA by lead compounds was demonstrated
using the cellular thermal shift assay (CETSA), and drug–target
residence time was determined via SPR. Analysis of these data suggests
that drug–target residence time (off-rate) may be an important
attribute to consider for obtaining potent cell-based inhibition of
this cancer metabolism target
Discovery and Optimization of Potent, Cell-Active Pyrazole-Based Inhibitors of Lactate Dehydrogenase (LDH)
We report the discovery and medicinal
chemistry optimization of
a novel series of pyrazole-based inhibitors of human lactate dehydrogenase
(LDH). Utilization of a quantitative high-throughput screening paradigm
facilitated hit identification, while structure-based design and multiparameter
optimization enabled the development of compounds with potent enzymatic
and cell-based inhibition of LDH enzymatic activity. Lead compounds
such as <b>63</b> exhibit low nM inhibition of both LDHA and
LDHB, submicromolar inhibition of lactate production, and inhibition
of glycolysis in MiaPaCa2 pancreatic cancer and A673 sarcoma cells.
Moreover, robust target engagement of LDHA by lead compounds was demonstrated
using the cellular thermal shift assay (CETSA), and drug–target
residence time was determined via SPR. Analysis of these data suggests
that drug–target residence time (off-rate) may be an important
attribute to consider for obtaining potent cell-based inhibition of
this cancer metabolism target