Human dental pulp stem cells produce mineralized matrix in 2D and 3D cultures

The aim of this study was to characterize the in vitro osteogenic differentiation of dental pulp stem cells (DPSCs) in 2D cultures and 3D biomaterials. DPSCs, separated from dental pulp by enzymatic digestion, and isolated by magnetic cell sorting were differentiated toward osteogenic lineage on 2D surface by using an osteogenic medium. During differentiation process, DPSCs express specific bone proteins like Runx-2, Osx, OPN and OCN with a sequential expression, analogous to those occurring during osteoblast differentiation, and produce extracellular calcium deposits. In order to differentiate cells in a 3D space that mimes the physiological environment, DPSCs were cultured in two distinct bioscaffolds, Matrigel™ and Collagen sponge. With the addition of a third dimension, osteogenic differentiation and mineralized extracellular matrix production significantly improved. In particular, in Matrigel™ DPSCs differentiated with osteoblast/osteocyte characteristics and connected by gap junction, and therefore formed calcified nodules with a 3D intercellular network. Furthermore, DPSCs differentiated in collagen sponge actively secrete human type I collagen micro-fibrils and form calcified matrix containing trabecular-like structures. These neo-formed DPSCs-scaffold devices may be used in regenerative surgical applications in order to resolve pathologies and traumas characterized by critical size bone defects

Utilizzo del laser a diodo nella terapia delle epulidi

The aim of this clinical research was to evaluate the use of diode laser in surgical treatments of oral and gingival epulis. This clinical study has been conducted in three dental clinics on a sample of 38 subjects, 13 of which had systemic immunodeficiency. This sample has been compared to a group of 56 subjects, showing the same oral lesion, treated by conventional surgical treatments. For both group the same research protocol and clinical follow-up schedule were chosen. Clinical results at short and long term were good. The diode laser surgical treatment of pyogenic granuloma of oral mucosa and periodontal tissues has shown a reduction of infective complications, a shorter recovery time interval and a significant diminution of relapses. In patients with HIV, HBV and HCV infections, the results of the research were similar to those obtained in patients without any viral infections

Laser e lesioni angiomatose del cavo orale.

Mardi 17 octobre 2017 9 h Accueil-Ouverture Pr. Luc-Thomas Somme, Recteur de l’Institut Catholique de Toulouse 9 h 30 Présentation du colloque Pr. Stéphane-Marie Morgain, CERES-Institut Catholique de Toulouse Génèse et premiers conflits d’interprétation Présidence : Madame Chrystel Bernat, Faculté de théologie protestante (Montpellier) 10 h Luther et les historiens, de Lucien Febvre à nos jours, Pr. Nicole Lemaitre, professeur émérite de l’Université Paris I-Panthéon-Sorbonne 10 h 40 Les cont..