Oxidative Stress Status and its correlation with Redox Status as Measured By An Electrochemical (PAOT®) Methodology: A Pilot Study in Critical COVID-19 Pneumonia Survivors

peer reviewe

A Pilot Study on Oxidative Stress during the Recovery Phase in Critical COVID-19 Patients in a Rehabilitation Facility: Potential Utility of the PAOT ® Technology for Assessing Total Anti-Oxidative Capacity

peer reviewedBackground: Oxidative stress (OS) could cause various COVID-19 complications. Recently, we have developed the Pouvoir AntiOxydant Total (PAOT®) technology for reflecting the total antioxidant capacity (TAC) of biological samples. We aimed to investigate systemic oxidative stress status (OSS) and to evaluate the utility of PAOT®for assessing TAC during the recovery phase in critical COVID-19 patients in a rehabilitation facility. Materials and Methods: In a total of 12 critical COVID-19 patients in rehabilitation, 19 plasma OSS biomarkers were measured: antioxidants, TAC, trace elements, oxidative damage to lipids, and inflammatory biomarkers. TAC level was measured in plasma, saliva, skin, and urine, using PAOT and expressed as PAOT-Plasma, -Saliva, -Skin, and -Urine scores, respectively. Plasma OSS biomarker levels were compared with levels from previous studies on hospitalized COVID-19 patients and with the reference population. Correlations between four PAOT scores and plasma OSS biomarker levels were analyzed. Results: During the recovery phase, plasma levels in antioxidants ( -tocopherol,  -carotene, total glutathione, vitamin C and thiol proteins) were significantly lower than reference intervals, whereas total hydroperoxides and myeloperoxidase (a marker of inflammation) were significantly higher. Copper negatively correlated with total hydroperoxides (r = 0.95, p = 0.001). A similar, deeply modified OSS was already observed in COVID-19 patients hospitalized in an intensive care unit. TAC evaluated in saliva, urine, and skin correlated negatively with copper and with plasma total hydroperoxides. To conclude, the systemic OSS, determined using a large number of biomarkers, was always significantly increased in cured COVID-19 patients during their recovery phase. The less costly evaluation of TAC using an electrochemical method could potentially represent a good alternative to the individual analysis of biomarkers linked to pro-oxidants

Les analyses bilologiques (extraction de l'ADN) et chimiques (détection des xénobiotiques) réalisées sur le cheveu et leurs applications

CHATENAY M.-PARIS 11-BU Pharma. (920192101) / SudocSudocFranceF

Vena cava inferior thrombosis diagnosed 12 years after interventional atrial septum defect closure in an infant.

editorial reviewe

Total absence of the pericardium associated with hypogammaglobulinemia and bronchiectasis in a girl

peer reviewe

A late diagnosis of vascular ring: right-sided aortic arch with Kommerell diverticulum and aberrant left subclavian artery in an adolescent.

peer reviewe

LaFeOxNy perovskite thin films: Nitrogen location and its effect on morphological, optical and structural properties

International audienc

Cell-free nucleic acids are present in blood products and regulate genes of innate immune response

Extracellular nucleic acids circulate in plasma. They are expected to be present in manufactured blood products eligible for transfusion, but little is known about their biological activity on human cells. The aim of this study is to investigate whether cell-free nucleic acids (CFNAs) are present and biologically active in red blood cell units (RBCUs), fresh frozen plasmas, and platelet concentrates

Regenerative Secretoma of Adipose-Derived Stem Cells from Ischemic Patients

Patients with terminal ischemia often reveal chronic limb and foot ulcers with subsequent risk of infection and/or amputation. Adipose-derived Stem Cells (ASC) may secrete angiogenic and regenerative factors. The autologous transplantation of such cells is considered to be an attractive therapeutic strategy, but their functional properties of ASC are influenced by many biochemical and biophysical stimuli of the microenvironment. Thus, patient-derived ASC might not be functionally competent. To study ASCs in ischemic disease, we have generated ASC lines from fat tissue of twelve ischemic patients. Lines were characterized for cell surface phenotype, multipotent capacities, and production of factors involved in wound healing. We succeeded to amplify ASC lines from all twelve patients and confirmed an ASC identity by their ability to: (i) adhere and grow on a plastic surface in standard culture conditions; (ii) express an ASC expression profile; (iii) differentiate in vitro into adipocytes, osteoblasts, and chondroblasts. Full transcriptome analysis of four selected lines showed a gene expression profile compatible with healing properties including all of the functional families involved in the wound healing process: extracellular matrix proteins, cell growth factors, pro-inflammatory cytokines, angiogenic factors, and matrix remodeling proteins. Our pilot study confirms that high-quality adipose stem cells can be easily derived from ischemic patients. Their transcriptome and secretome show a regenerative profile which makes them promising candidates for autologous therapy of chronic ulcers