Changes in body weight (A), energy intake (B), food intake (C), water intake (D), fat mass (E) and lean mass (F) in control fed mice (■) and HFFD fed mice (▲).

<p>Data are means ± SEM for 36 mice per group at 1, 2 and 3 months, 24 mice per group at 6 months and 12 mice per group at 8 months. *P≤0.05, **P≤0.01, ***P≤0.001.</p

Macrophage infiltration (A) and collagen accumulation (B) in HFFD fed mice (■) compared to control fed mice (▲)

<p>Data are means ± SEM for 12 mice per group; *p=0.0217. Pictures for macrophage staining display representative areas of kidneys from control or HFFD fed mice. Magnification x20. Pictures for collagen staining display representative tubulointerstitium of kidneys from control or HFFD fed mice. Magnification x20.</p

Glomerular hyperfiltration (A), urinary albumin to creatinine ratio (ACR) (B) and arterial blood pressure (C) in HFFD fed mice (■) compared to control fed mice (▲)

<p>Data are means ± SEM for 9 mice per group; *p=0.0429; **p=0.004.</p

Absence of mesangial matrix expansion in HFFD fed mice (■) compared to control fed mice (▲)

<p>Mesangial matrix expansion is evaluated by the ratio of the matrix surface to the total surface of the glomerulus after Periodic Acid Staining. Data are means ± SEM for 12 mice per group; <i>ns</i>. Pictures display representative glomeruli of kidneys from control or HFFD fed mice. Magnification x40.</p

Plasma level of triglycerides (A), cholesterol (B), HDL (C), LDL (D) and glucose (E) in control fed mice (■) and HFFD fed mice (▲)

<p>Data are means ± SEM for 12 mice per group. *P≤0.05, **P≤0.01, ***P≤0.001.</p