An endometrial histomorphometric study of CD56+ natural killer cells in women with unexplained infertility

BACKGROUND. The number of peripheral blood and endometrial natural killer cells varies greatly during implantation and the first trimester of pregnancy and is thought to play a role in the maintenance of a healthy pregnancy. However, the role of endometrial CD56+ natural killer (NK) cells as an immunological mechanism in unexplained infertility is yet unknown. OBJECTIVES. The study aimed to enumerate the concentrations of CD56+ NK cells in endometrial samples, and to statistically compare these numbers between fertile and infertile women. METHODS. A histomorphometric analysis was conducted using haematoxylin and eosin staining and an immunohistochemical approach was used for quantifying cell numbers. RESULTS. Fifty samples were collected in equal parts between a study group of infertile female subjects (mean (standard deviation) age 35 (4), range 26 - 42 years) and a control group of multiparous fertile individuals (mean (SD) age 43.4 (6.3), range 30 - 55). The mean number of CD56+ NK cells present at different depths for both the study and control groups did not differ significantly. Age and group (study or control) were not significantly related to the mean number of CD56+ NK cells. However, for the late secretory phase the mean number of CD56+ NK cells was significantly higher than for the early phase. CONCLUSION. Our findings could not identify a statistically significant correlation between the number of CD56+ NK cells and infertility.Discovery Foundation Research Awardshttp://sajog.org.za/index.php/SAJOGhttp://www.journals.co.za/content/journalam2017Anatomical PathologyAnatomyHaematologyImmunologyStatistic

High tie versus low tie in rectal surgery: comparison of anastomotic perfusion

Item does not contain fulltextPURPOSE: Both "high tie" (HT) and "low tie" (LT) are well-known strategies in rectal surgery. The aim of this study was to compare colonic perfusion after HT to colonic perfusion after LT. METHODS: Patients undergoing rectal resection for malignancy were included. Colonic perfusion was measured with laser Doppler flowmetry, immediately after laparotomy on the antimesenterial side of the colon segment that was to become the afferent loop (measurement A). This measurement was repeated after rectal resection (measurement B). The blood flow ratios (B/A) were compared between the HT group and the LT group. RESULTS: Blood flow was measured in 33 patients, 16 undergoing HT and 17 undergoing LT. Colonic blood flow slightly decreased in the HT group whereas the flow increased in the LT group. The blood flow ratio was significantly higher in the LT group (1.48 vs. 0.91; p = 0.04), independent of the blood pressure. CONCLUSION: This study shows the blood flow ratio to be higher in the LT group. This suggests that anastomoses may benefit from better perfusion when LT is performed

Polar Data Forum IV – An Ocean of Opportunities

This paper reports on the Hackathon Sessions organised at the Polar Data Forum IV (PDF IV) (20–24 September 2021), during which 351 participants from 50 different countries discussed collaboratively about the latest developments in polar data management. The 4th edition of the PDF hosted lively discussions on (i) best practices for polar data management, (ii) data policy, (ii) documenting data flows into aggregators, (iv) data interoperability, (v) polar federated search, (vi) semantics and vocabularies, (vii) Virtual Research Environments (VREs), and (viii) new polar technologies. This paper provides an overview of the organisational aspects of PDF IV and summarises the polar data objectives and outcomes by describing the conclusions drawn from the Hackathon Sessions

Amino-terminal pro-brain natriuretic peptide (NT-proBNP) is a biomarker of cardiac filling pressures in pre-eclampsia

Objective To evaluate if amino-terminal pro-brain natriuretic peptide (NT-proBNP) plasma levels reflect intracardiac filling pressures in pre-eclamptic patients Study design In a cross-sectional study we investigated 22 untreated critically ill pre-eclamptic women between 22 and 34 weeks gestation All patients underwent intra-arterial blood pressure and central hemodynamic measurements and NT-proBNP was determined in stored plasma Baseline characteristics plasma NT-proBNP concentrations and relevant laboratory variables were investigated for correlations with hemodynamic values using Spearman s rank correlation test Results No significant correlations were demonstrated between NT-proBNP concentrations and variables associated with the severity of the pre-eclampsia We found significant positive correlations between NT-proBNP and diastolic pulmonary pressure (r = 0 59 p = 0 005) and pulmonary capillary wedge pressure (PCWP) (r = 051 p = 0 015) Multiple linear regression analysis showed that the association between NT-proBNP and PCWP was not affected by creatinine level Conclusion NT-proBNP is a blomarker of left ventricular cardiac filling pressures in untreated pre-eclamptic patients (C) 2010 Elsevier Ireland Ltd All rights reserve

Sweet spot identification in underexplored shales using multidisciplinary reservoir characterization and key performance indicators: Example of the Posidonia Shale Formation in the Netherlands

Sweet spot identification in underexplored shale gas basins needs to be based on a limited amount of data on shale properties in combination with upfront geological characterization and modelling, because actual production data is usually absent. Multidisciplinary reservoir characterization and integration of modelling approaches can aid initial site selection for exploratory drilling and de-risk exploration efforts. In this study, the potential of hydrocarbon production across underexplored shale basins is analyzed using key performance indicators. A mean indicator is defined as the harmonic mean of three performance indicators that describe the potential for hydrocarbon generation, storage, and flow stimulation. The performance indicators are based on a limited number of local shale properties, i.e. vitrinite reflectance, porosity, depth, thickness, sorbed gas content and brittleness index. Values for the indicators are calculated relative to prospective North American shales (Marcellus, Bakken, Haynesville and Barnett), so that the mean indicator can be used to rank the prospectivity of an underexplored shale relative to hydrocarbon producing shales. The mean performance indicator is also used to map out the potential for gas production in the underexplored Posidonia Shale Formation in the West Netherlands Basin. The analysis shows that the performance indicator is lower for the Posidonia than for the North American Shales, mainly due to low maturity and brittleness. Local maxima of the indicator correlate with local maxima in vitrinite reflectance and depth. Besides the low potential for flow stimulation, the potential for hydrocarbon generation and storage capacity of the Posidonia are comparable to oil-window thermal-maturity Barnett Shale. Upfront simulations of hydraulic fracture properties and gas production in the Posidonia Shale Formation are in rough agreement with observed average gas flow for Barnett wells in non-core areas that are oil mature. It shows that key performance indicators can be applied to underexplored shales to quantify prospectivity and map out sweet spots across shale basins