Impact of UV radiation on DOM transformation on molecular level using FT-ICR-MS and PARAFAC

Dissolved organic matter (DOM) is an omnipresent constituent of natural water bodies. Reuse and transformation of DOM compounds in the water column is driven by physicochemical and biological processes leading to the production of refractory DOM. Typically, breakdown of DOM chemical compounds into smaller or more condensed fragments is triggered by ultraviolet (UV) radiation. Here, we present a study on the photodegradation of DOM produced during an incubation experiment with a natural microbial community. At the end of the first incubation without UV irradiation, the samples from 3 mesocosms were filtered to remove microbes and particles and continuously exposed to UV radiation (280–365 nm). We investigated DOM in depth via monitoring of dissolved organic carbon (DOC) concentrations, DOM molecular characterization by Fourier-Transform Ion Cyclotron Resonance Mass Spectrometry (FT-ICR-MS) and excitation emission matrix spectroscopy (EEMS). Analysis of variance indicated no significant differences in the DOC concentration between treatments. Main peaks in the fluorescent DOM (FDOM) were photo-bleached by UV radiation, and an increase in the fluorescent intensity of selected peaks was observed on irradiated samples toward the end of the experiment. Parallel factor analysis (PARAFAC) indicated the presence of three main components in all treatments: C1 (Marine humic M), C2 (Bacterial produced humic C), C3 (Tyrosine), and an additional component in the dark incubation of mesocosm 3, C4 (Tryptophan). Despite an intensive filtration protocol through 0.7, 0.2 and 0.1 μm filters, low bacterial abundances were determined (<2.5 × 10−3 cells mL−1). We observed a direct correlation between structural indices and the intensity of PARAFAC components. Average double bond equivalent and aromaticity were strongly positively correlated with PARAFAC components C1 and C2 for one or more mesocosm. Moreover, FT-ICR-MS showed that under the tested conditions, the refractory character of the DOM assessed as the similarity to a deep ocean DOM reference did not increase on molecular level. Thus, mechanisms other than photochemical transformations of relatively recent DOM are likely necessary to facilitate long-term stability of DOM in the oceans

Signatures of bosonic squark decays in non-minimally flavour-violating supersymmetry

We investigate couplings of squarks to gauge and Higgs-bosons within the framework of non-minimal flavour violation in the Minimal Supersymmetric Standard Model. Introducing non-diagonal elements in the mass matrices of squarks, we first study their impact on the self-energies and physical mass eigenvalues of squarks. We then present an extensive analysis of bosonic squark decays for variations of the flavour-violating parameters around the two benchmark scenarios SPS1a' and SPS1b. Signatures, that would be characteristic for a non-minimal flavour structure in the squark sector, can be found in wide regions of the parameter space.We thank T. Hurth for useful discussions and F. Staub for cross checking the formulae given in appendix B. This work has been supported by the DFG, project number PO-1337/1-1. B.H. acknowledges support from the Hamburg Excellence Cluster "Connecting particles to the cosmos" and W.P. from the Alexander von Humboldt Foundation and the Spanish grant FPA2008-00319/FPA.Peer reviewe