Mutation pattern is not effected by sample collection.

<p><b>(A-B)</b> Lego plot of single-nucleotide variants plotted by sequence context for variants with allelic fractions > 0.05 and < 0.95. Samples collected in <b>A)</b> EDTA tubes and <b>B)</b> Streck tubes were combined and plots show the sum of the three samples.</p

Microarray results.

<p>Microarray results.</p

WES variant calls.

<p>WES variant calls.</p

DNA extraction and sequencing metrics.

<p>DNA extraction and sequencing metrics.</p

Sequencing coverage is not effected by sample collection tube.

<p><b>(A-B)</b> Box plots of the read coverage for target regions binned by GC content for samples collected in <b>A)</b> EDTA tubes or <b>B)</b> Streck tubes. For all box plots, the central box indicates values in the range 25^th-75^th percentile of all values for that subset of data with the central line indicating the median. Whiskers extend 1.5x from the lower and upper boundaries of the central box with points outside that range indicated as black circles.</p