AMOVA of microsatellite data, divided according to population, with all ten populations included.

1<p>d.f.: degrees of freedom;</p>2<p>SS: sum of squares;</p>3<p>MS: mean squares;</p>4<p>p: significance level.</p

AMOVA of microsatellite data, divided according to ecotype and population, with the seven unambiguously classified populations included.

1<p>d.f.: degrees of freedom;</p>2<p>SS: sum of squares;</p>3<p>MS: mean squares;</p>4<p>p: significance level.</p

The percentage of individuals from the two ecotypes and the three intermediate populations (rows) that were classified as members of each ecotype (columns) in the discriminant analysis of the principal coordinate data.

<p>The percentage of individuals from the two ecotypes and the three intermediate populations (rows) that were classified as members of each ecotype (columns) in the discriminant analysis of the principal coordinate data.</p

Summary statistics for each locus.

1<p><i>N</i>: number of alleles.</p

The percentage of individuals from the various populations (rows) that were classified as members of each population (columns) in the discriminant analysis of the principal coordinate data.

<p>The percentage of individuals from the various populations (rows) that were classified as members of each population (columns) in the discriminant analysis of the principal coordinate data.</p

Grindelia_allele_frequencies

This is a tab-delimited text file with the information on which alleles were present in each individual. In total, there are data from six microsatellite loci (columns) and 439 individuals (rows). Loci and individuals are identified in a header row or column, respectively. Data were gathered from wild-collected leaf material and scored using PeakScanner v. 1.0

Representative plots from the <i>structure</i> analysis, with genotypes assumed to be unambiguous.

<p><i>K</i> is the number of groups into which the plants were divided.</p

Plots of the populations under three separate analyses.

<p>The percent of the variance explained by the first two axes is shown for each analysis. (A) Population centroids from the Principal Coordinates Analysis of the analysis of all individuals using Dice’s similarity index; 15.7% of the variance explained. (B) Population centroids from the discriminant analysis of the Principal Coordinates data; 46.4% of the variance explained. (C) Principal Coordinates Analysis of the ρ data calculated for each population; 66% of the variance explained. Symbols follow <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0095656#pone-0095656-g001" target="_blank">Figure 1</a>.</p

Appendix B. Extended table summarizing the estimates of phylogenetic signal (Blomberg's K statistic) and tests of significance for niche parameters not included in Table 4.

Extended table summarizing the estimates of phylogenetic signal (Blomberg's K statistic) and tests of significance for niche parameters not included in Table 4

Appendix A. Sampling protocol, site names, and GPS coordinates for vegetation sampling that estimated the depth of occurrence for Lasthenia lineages occupying vernal pools.

Sampling protocol, site names, and GPS coordinates for vegetation sampling that estimated the depth of occurrence for Lasthenia lineages occupying vernal pools