Multiplexed Nanometric 3D Tracking of Microbeads using a FFT-Phasor Algortithm

Biological and Soft Matter Physic

Toehold-enchanced lna probes for selective pull down and single-molecule analysis of native chromatin

Biological and Soft Matter Physic

Single-molecule force spectroscopy on histone h4 tail cross-linked chromatin reveals fiber folding

Biological and Soft Matter Physic

Arts handbook

1998 handbook for the faculty of Art

Toehold-enchanced lna probes for selective pull down and single-molecule analysis of native chromatin

Biological and Soft Matter Physic

A critical role for linker DNA in higher-order folding of chromatin fibers

Biological and Soft Matter Physic

(A)Specific DNA binding of archaeal histones, the formation and positioning of hypernucleosomes

Biological and Soft Matter Physic

The role of linker DNA in chromatin fibers

The genetic information of all living organisms is contained in their DNA. Cells modify the degree of DNA compaction by epigenetics, which largely determines what genes are read out and which genes are transcriptionally silent. Despite decades of research into this mechanism, there is no consensus on how cells realize the various degrees of DNA compaction in vivo. Eukaryotes, such as humans, compact their DNA into higher-order structures called compact chromatin fibers. We characterize these fibers through a combination of single-molecule force spectroscopy techniques like magnetic tweezers, and rigid base pair Monte Carlo simulations. We show that, for instance, the length and sequence of the linker DNA, the DNA between adjacent nucleosomes, control the mechanical properties of chromatin fibers.  Our measurements suggest the formation of more than one higher-order fiber structure. A deeper understanding of the chromatin fiber and its compaction mechanism is important because the dysfunction of such regulation results in various medical conditions such as the epigenetic disorder type 1 diabetes, fragile X syndrome, or various cancers.Biological and Soft Matter Physic

Multiplexed nanometric 3D tracking of microbeads using an FFT-phasor algorithm

Biological and Soft Matter Physic