Letter from James B. Brooke & M.F. Brooke to James B. Finley

Grandson James warns Finley that he should take care of his health since he is no longer a young man. He apologizes for not having written sooner and then writes news of the family (wife is Margaret Fisher Brooke). Many have suffered from bilious attacks but are generally well now. He plans to go home about the first of April. Abstract Number - 275https://digitalcommons.owu.edu/finley-letters/1273/thumbnail.jp

Letter from James B. Brooke & M.F. Brooke to James B. Finley

The Brookes have suffered a great deal of ill health during the past fall. They write to thank Finley for his kind letters and remembrances. Abstract Number - 354https://digitalcommons.owu.edu/finley-letters/1552/thumbnail.jp

Letter from James B. Brooke to James B. Finley

Finley\u27s grandson James has had some difficulty paying off Finley\u27s taxes for him. Finley had given his grandson $10 to liquidate a tax bill after learning he was delinquent, but by the time James got to Greenville to pay it off, the tax had been increased to$14.26. The treasurer insisted that the whole amount be paid at once, so James could not pay the $10. He then learned that there were back taxes owed for 1846. He has had to work very hard but he finally has the bill paid. Abstract Number - 308https://digitalcommons.owu.edu/finley-letters/1508/thumbnail.jp

Letter from James B. Brooke to James B. Finley

Finley\u27s grandson James tells first of an epidemic of fever which affected his whole family and caused the deaths of many others. He then relates plans to build a Methodist church in his vicinity. He would like to donate the shingles but cannot do it alone, so asks Finley to help him by soliciting $15 or$20 in his territory. The church is very necessary, he says, due to the ignorance and vice prevalent in the town. Abstract Number - 406https://digitalcommons.owu.edu/finley-letters/1604/thumbnail.jp

Letter from James B. Brooke to James B. Finley

Finley\u27s grandson James reports on the death of Elizabeth Dolley on January 24th. James\u27 business partner plans to go to California to seek gold. James needs $150 to buy him out. He is still attempting to find a lot for the new Meeting House. Abstract Number - 423https://digitalcommons.owu.edu/finley-letters/1621/thumbnail.jp

Letter from James B. Brooke to James B. Finley

Grandson James expresses the hope that Finley\u27s health is improving. He is working very hard, is nervous, and finds it difficult to write. His letter has a strong religious tone -- preparation for life after death. He plans to move to Castine next winter and start a potting business. Abstract Number - 328https://digitalcommons.owu.edu/finley-letters/1528/thumbnail.jp

A Role for Actin, Cdc1p, and Myo2p in the Inheritance of Late Golgi Elements in \u3cem\u3eSaccharomyces cerevisiae\u3c/em\u3e

In Saccharomyces cerevisiae, Golgi elements are present in the bud very early in the cell cycle. We have analyzed this Golgi inheritance process using fluorescence microscopy and genetics. In rapidly growing cells, late Golgi elements show an actin-dependent concentration at sites of polarized growth. Late Golgi elements are apparently transported into the bud along actin cables and are also retained in the bud by a mechanism that may involve actin. A visual screen for mutants defective in the inheritance of late Golgi elements yielded multiple alleles of CDC1. Mutations in CDC1 severely depolarize the actin cytoskeleton, and these mutations prevent late Golgi elements from being retained in the bud. The efficient localization of late Golgi elements to the bud requires the type V myosin Myo2p, further suggesting that actin plays a role in Golgi inheritance. Surprisingly, early and late Golgi elements are inherited by different pathways, with early Golgi elements localizing to the bud in a Cdc1p- and Myo2p-independent manner. We propose that early Golgi elements arise from ER membranes that are present in the bud. These two pathways of Golgi inheritance in S. cerevisiae resemble Golgi inheritance pathways in vertebrate cells

Optimizing high-flow nasal cannula flow settings in adult hypoxemic patients based on peak inspiratory flow during tidal breathing

Cànula nasal d'alt flux; Hipoxèmia; Flux inspiratori màximCánula nasal de alto flujo; Hipoxemia; Flujo inspiratorio máximoHigh-flow nasal cannula; Hypoxemia; Peak inspiratory flowBackground Optimal flow settings during high-flow nasal cannula (HFNC) therapy are unknown. We investigated the optimal flow settings during HFNC therapy based on breathing pattern and tidal inspiratory flows in patients with acute hypoxemic respiratory failure (AHRF). Methods We conducted a prospective clinical study in adult hypoxemic patients treated by HFNC with a fraction of inspired oxygen (FIO2) ≥ 0.4. Patient’s peak tidal inspiratory flow (PTIF) was measured and HFNC flows were set to match individual PTIF and then increased by 10 L/min every 5–10 min up to 60 L/min. FIO2 was titrated to maintain pulse oximetry (SpO2) of 90–97%. SpO2/FIO2, respiratory rate (RR), ROX index [(SpO2/FIO2)/RR], and patient comfort were recorded after 5–10 min on each setting. We also conducted an in vitro study to explore the relationship between the HFNC flows and the tracheal FIO2, peak inspiratory and expiratory pressures. Results Forty-nine patients aged 58.0 (SD 14.1) years were enrolled. At enrollment, HFNC flow was set at 45 (38, 50) L/min, with an FIO2 at 0.62 (0.16) to obtain an SpO2/FIO2 of 160 (40). Mean PTIF was 34 (9) L/min. An increase in HFNC flows up to two times of the individual patient’s PTIF, incrementally improved oxygenation but the ROX index plateaued with HFNC flows of 1.34–1.67 times the individual PTIF. In the in vitro study, when the HFNC flow was set higher than PTIF, tracheal peak inspiratory and expiratory pressures increased as HFNC flow increased but the FIO2 did not change. Conclusion Mean PTIF values in most patients with AHRF were between 30 and 40 L/min. We observed improvement in oxygenation with HFNC flows set above patient PTIF. Thus, a pragmatic approach to set optimal flows in patients with AHRF would be to initiate HFNC flow at 40 L/min and titrate the flow based on improvement in ROX index and patient tolerance.This study does not have funding/support