Genotypic characterization of Escherichia coli strains isolated from dairy cattle environment

The aim of this study was to characterize the diversity of Escherichia coli strains involved in the dispersion of virulence genes. 152 E. coli strains originated from dairy cattle environment were evaluated through phenotypic and proteomic assays. These samples were investigated for the presence of virulence genes (eaeA, stxI, stxII, ST, LT, eagg, ial) and biofilm related genes (fimH, csgA, flu)Fil: Bronzato, Greiciane França. Universidade Federal Rural Do Rio de Janeiro; BrasilFil: Bento Rodrigues, Naiara Miranda. Universidade Federal Rural Do Rio de Janeiro; BrasilFil: Pribul, Bruno Rocha. Oswaldo Cruz Institute. National Reference Laboratory for Enteric Diseases; BrasilFil: Stefaninni, Gabrielli. Universidade Federal Rural Do Rio de Janeiro; BrasilFil: Coelho, Irene da Silva. Universidade Federal Rural Do Rio de Janeiro; BrasilFil: Soares De Souza, Miliane Moreira. Universidade Federal Rural Do Rio de Janeiro; BrasilFil: Reinoso, Elina Beatríz. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; ArgentinaFil: Lasagno, Mirta Cristina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; ArgentinaFil: Oliveira Coelho, Shana de Mattos de. Universidade Federal Rural Do Rio de Janeiro; Brasi

Detection of virulence and antibiotic resistance genes in environmental strains of Vibrio spp. from mussels along the coast of Rio de Janeiro State, Brazil

Submitted by Sandra Infurna ([email protected]) on 2017-02-23T14:45:43Z No. of bitstreams: 1 bruno_pribul_etal_IOC_2016.pdf: 490720 bytes, checksum: 4cbf93f67ecf0d00238bbf8b1621c0aa (MD5)Approved for entry into archive by Sandra Infurna ([email protected]) on 2017-02-23T15:00:42Z (GMT) No. of bitstreams: 1 bruno_pribul_etal_IOC_2016.pdf: 490720 bytes, checksum: 4cbf93f67ecf0d00238bbf8b1621c0aa (MD5)Made available in DSpace on 2017-02-23T15:00:42Z (GMT). No. of bitstreams: 1 bruno_pribul_etal_IOC_2016.pdf: 490720 bytes, checksum: 4cbf93f67ecf0d00238bbf8b1621c0aa (MD5) Previous issue date: 2016Universidade Federal Rural do Rio de Janeiro. Instituto Veterinário. Departamento de Microbiologia e Imunologia Veterinária. Seropédica, RJ, Brasil.Universidade Federal Rural do Rio de Janeiro. Instituro Vetrinário. Departamento de Microbiologia e Imunologia veterinária. Seropédica, RJ, Brasil.Universidade Severino Sombra. Vassouras, RJ, Brasil.Universidade Federal do Rio de Janeiro. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil.Universidade Severino Sombra. Vassouras, RJ, Brasil.Universidade Federal Rural do Rio de Janeiro. Instituto Veterinário. Departamento de Microbiologia e Imunologia Veterinária. Seropédica, RJ, Brasil.Universidade Federal Rural do Rio de Janeiro. Instituto Veterinário. Departamento de Microbiologia e Imunologia Veterinária. Seropédica, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil.Universidade Federal Rural do Rio de Janeiro. Instituto Veterinário. Departamento de Microbiologia e Imunologia Veterinária. Seropédica, RJ, Brasil.Mussels have a filter system enabling them to take up nutrients from the water, so a microbiological analysis of these bivalve mollusks can show the contamination levels of their surrounding aquatic environment. The present work aimed to isolate Vibrio species from two hundred samples of mussels (Perna perna) incrusted on rocks of the Santana Archipelago and from longline mariculture in Ilha Grande Bay in Angra dos Reis and from Arraial do Cabo, all of which are in Rio de Janeiro state, Brazil. A total of 209 Vibrio were isolated. The most prevalent species was Vibrio parahaemolyticus (44.66%) followed by Vibrio alginolyticus (19.62%) and Vibrio vulnificus (12.44%). All 209 Vibrio isolates tested positive for the RNA polymerase alpha gene (rpoA). The tlh gene (thermolabile hemolysin), a genetic marker for V. parahaemolyticus, and vvhA (cytolysin hemolysin) of V. vulnificus were detected in 85 and 26 isolates, respectively. The MALDI-TOF MS proteomic technique was used to confirm the identification of the 41 V. alginolyticus isolates. Our most important finding was the detection of the tdh virulence gene in 68.20% (58/85) of V. parahaemolyticus environmental strains. Besides the circulation of the virulence gene, the spread of antimicrobial resistance was evaluated and 91.3% (191/209) of the isolates showed resistance to ampicillin, 23.9% (50/209) to ciprofloxacin, 18.6% (39/209) to nitrofurantoin, 5.7% (12/209) to tetracycline, 4.3% (9/209) to pefloxacin and 3.3% (7/209) to chloramphenicol. These findings indicate that environmental isolates can act as reservoirs of virulence and antibiotic resistance genes