Isolation And Characterization Of Microsatellite Markers In The New World Screw-worm Cochliomyia Hominivorax (diptera: Calliphoridae)

The new world screw-worm, Cochliomyia hominivorax, is one of the most important parasitic insect pests. This species causes myiasis and, consequently, significant losses to livestock industries in neotropical regions. As part of an investigation into the genetic variability and population structure of the new world screw-worm, a set of 10 polymorphic microsatellite markers was isolated from an AC-enriched genomic library. Amplification of the reported loci in 30 screw-worms revealed an average of 6.9 alleles per locus with expected heterozygosities ranging from 0.3831 to 0.8022. Cross-species amplifications suggested that these loci may be useful in other Calliphoridae species.42182184Brondani, R.P.V., Brondani, C., Tarchini, R., Grattapaglia, D., Development, characterization and mapping of microsatellite markers in Eucalyptus grandis and E. urophyla (1998) Theoretical and Applied Genetics, 97, pp. 816-827(2000) Genetic Sexing and Population Genetics of Screwworms, , August 7-11, 2000. International Atomic Energy Agency, Vienna, AustriaLa Chance, L.E., Bartlett, A.C., Bram, R.A., Mating types in screwworm populations? (1982) Science, 218, pp. 1142-1143Raymond, M., Rousset, F., GENEPOP (version 1.2): Population genetics software for exact tests and ecumenicism (1995) Journal of Heredity, 86, pp. 248-249Rozen, S., Skaletsky, H.J., (2000) PRIMER 3, , http://www-genome.wi.mit.edu/genome_software/other/primer3.htmlWyss, J.H., Galvin, T.J., Central America regional screwworm eradication program (benefit/cost study) (1996) Annals of the New York Academy of Sciences, 791, pp. 241-24