2 research outputs found
Versatile and User-Friendly Anti-infective Hydrogel for Effective Wound Healing
Wound dressings play a crucial role
in facilitating optimal
wound
healing and protecting against microbial infections. However, existing
commercial options often fall short in addressing chronic infections
due to antibiotic resistance and the limited spectrum of activity
against both Gram-positive and Gram-negative bacteria frequently encountered
at wound sites. Additionally, complex fabrication processes and cumbersome
administration strategies pose challenges for cost-effective wound
dressing development. Consequently, there is a pressing need to explore
easily engineered biocompatible biomaterials as alternative solutions
to combat these challenging wound infections. In this study, we present
the development of an anti-infective hydrogel, P-BAC (polymeric bactericidal
hydrogel), which exhibits simple administration and promotes efficient
wound healing. P-BAC is synthesized via a one-step fabrication method
that involves the noncovalent cross-linking of poly(vinyl alcohol), N-(2-hydroxypropyl)-3-trimethylammonium chitosan chloride-AgCl
nanocomposite, and proline. Remarkably, P-BAC demonstrates broad-spectrum
antibacterial activity against both planktonic and stationary cells
of clinically isolated Gram-positive and Gram-negative bacteria, resulting
in a significant reduction of bacterial load (5–7 log reduction).
Moreover, P-BAC exhibits excellent efficacy in eradicating bacterial
cells within biofilm matrices (>95% reduction). In vivo experiments
reveal that P-BAC accelerates wound healing by stimulating rapid collagen
deposition at the wound site and effectively inactivates ∼95%
of Pseudomonas aeruginosa cells. Importantly,
the shear-thinning property of P-BAC simplifies the administration
process, enhancing its practicality and usability. Taken together,
our findings demonstrate the potential of this easily administrable
hydrogel as a versatile solution for effective wound healing with
potent anti-infective properties. The developed hydrogel holds promise
for applications in diverse healthcare settings, addressing the critical
need for improved wound dressing materials
Dual Function Injectable Hydrogel for Controlled Release of Antibiotic and Local Antibacterial Therapy
We
present vancomycin-loaded dual-function injectable hydrogel
that delivers antibiotic locally suitable for treatment of infections
in avascular or necrotic tissues. The syringe-deliverable gels were
developed using polydextran aldehyde and an inherently antibacterial
polymer <i>N</i>-(2-hydroxypropyl)-3-trimethylammonium chitosan
chloride along with vancomycin. The antibiotic was primarily encapsulated
via reversible imine bonds formed between vancomycin and polydextran
aldehyde in the hydrogel which allowed sustained release of vancomycin
over an extended period of time in a pH-dependent manner. Being inherently
antibacterial, the gels displayed excellent efficacy against bacteria
due to dual mode of action (killing bacteria upon contact as well
as by releasing antibiotics into surroundings). Upon subcutaneous
implantation, the gel was shown to kill methicillin-resistant Staphylococcus aureus (>99.999%) when bacteria
were
introduced directly into the gel as well as at distal site from the
gel in a mice model. These materials thus represent as novel noninvasive
drug-delivery device suitable for local antibiotic therapy