Primers used for PCR detection of genes encoding virulence factors.

<p>Other virulence factors have been detected by multiplex PCR onto purified total DNA (Qiagen). Presence of genes encoding enterotoxins E, G, H, K, L, T, epidermolysin D (Etd), genes encoding Edin A and EDIN B and the genes encoding seven adhsesion factors (Cna, FnbA, FnbB Bbp, Clfb, Fib, Ebp and Lbp) was checked in 8 set in function of base size.</p

Pulsed field gel electrophoresis (PFGE) dependent dendogram of isolated <i>Staphylococcus aureus</i>.

<p>Pulsed field gel electrophoresis (PFGE) proves no specific clonal relationship between PVL-producing isolates issued from furuncles or secondary dermatosis. The similarity of the different pulsotypes was established by using Molecular Analyst™ software. Twenty four pulsotypes corresponded to the 55 isolates and their distribution is given according to the groups of isolates issued from secondary infected dermatosis (1), furuncles from HIV (−)(2) or HIV (+)(3) patients.</p

Production of toxins and identification of genes encoding toxins in <i>Staphylococcus aureus</i>.

<p>The majority of <i>SA</i> strains isolated from HIV patient-derived furuncles significantly produced PVL (<i>p</i><0.05), whereas only 10% of <i>SA</i> strains produced this toxin in secondary infected dermatosis. A high prevalence of LukE-LukD-producing isolates (56 to 78%) was recorded in patient groups.</p

Supplementary data from Response of CO₂-starved diatom <i>Phaeodactylum tricornutum</i> to light intensity transition

In this study, we investigated the responses of <i>Phaeodactylum tricornutum</i> cells acclimated to 300 µmol m⁻² s⁻¹ photon flux density to an increase (1000 µmol m⁻² s⁻¹) or decrease (30 µmol m⁻²s⁻¹) photon flux densities. The light shift occurred abruptly after 5 days of growth and the acclimation to new conditions was followed during the next 6 days at the physiological and molecular levels. The molecular data reflect a rearrangement of carbon metabolism towards the production of PEP and/or pyruvate. These intermediates were used differently by the cell as a function of the photon flux density: under low light, the photosynthesis was depressed while respiration was increased. Under high light, lipids and proteins accumulated. Of great interest, under high light, the genes coding for the synthesis of aromatic amino acids and phenolic compounds were upregulated suggesting that the Shikimate pathway was activated.This article is part of the themed issue ‘The peculiar carbon metabolism in diatoms’