Copy number evaluation by Fluorescence <i>In Situ</i> Hybridization (FISH) of the loci carrying <i>hTERC</i> and <i>hTERT</i> in late chorionic villus samplings.

<p>A. Percentage of interphase nuclei displaying no gain or loss, relative losses, relative gains, and absolute gains of <i>hTERC</i> locus with the FISH technique ± standard deviation (%) for 12 controls (light grey histograms) and 12 cases with intrauterine growth restriction (IUGR) (dark grey histograms) randomly selected (<i>p</i> = non-significant (NS), Student’s t-test). B. Percentage of interphase nuclei displaying no gain or loss, relative losses, relative gains, and absolute gains of <i>hTERT</i> locus with the FISH technique ± standard deviation (%) for 12 controls (light grey histograms) and 12 cases with IUGR (dark grey histograms) randomly selected (<i>p</i> = NS, Student’s t-test).</p

Main characteristics of the subjects and their pregnancies.

<p>AU: arbitrary units; IUGR; intrauterine growth restriction; MSS; second trimester maternal serum screening; US: ultrasound.</p

Comparison of placental telomere length between controls and cases with intrauterine growth restriction (IUGR), with two quantitative techniques.

<p>A. Quantitative Fluorescence <i>In Situ</i> Hybridization (Q-FISH). Mean placental telomere fluorescence intensity of Peptide Nucleic Acid probes with the Q-FISH technique ± standard deviation (arbitrary units) for the 28 controls (light grey histogram) and the 24 cases with IUGR (dark grey histogram) (**: <i>p</i><0.001, Student’s t-test). B. Quantitative Polymerase Chain Reaction (Q-PCR). Mean placental telomere T/S ratio with the Q-PCR technique ± standard deviation (no unit) for the 28 controls (light grey histogram) and the 24 cases with IUGR (dark grey histogram) (**: <i>p</i><0.001, Student’s t-test).</p