8 research outputs found
Generation of stable Xenopus laevis transgenic lines expressing a transgene controlled by weak promoters.
International audienceCombining two existing protocols of trangenesis, namely the REMI and the I-SceI meganuclease methods, we generated Xenopus leavis expressing a transgene under the control of a promoter that presented a restricted pattern of activity and a low level of expression. This was realized by co-incubating sperm nuclei, the I-SceI enzyme and the transgene prior to transplantation into unfertilized eggs. The addition of the woodchuck hepatitis virus posttranscriptional regulatory element in our constructs further enhanced the expression of the transgene without affecting the tissue-specificity of the promoter activity. Using this combination of methods we produced high rates of fully transgenic animals that stably transmitted the transgene to the next generations with a transmission rate of 50% indicating a single integration event
Terminal structure of hypovirulence-associated dsRNAs in the chestnut blight fungus Endothia parasitica
La plate-forme RIO "Transgenèse chez le xénope": Quelques exemples d'application.
Communication orale présentée par T. Madigo
Spatio-temporal cellular expression of NeuroD and related cells properties in transgenic /Xenopus laevis/ tadpoles and froglets
Poste
Xenopus transgenic embryos to study early neuronal differentiation
Poster présenté par A. Guidet-L'hosti
The Woodchuck Post-transcriptional Regulatory Element (WPRE) successfully enhances transgene expression level in Xenopus leavis
Communication oral