1,005 research outputs found
Essential Role of the Iron-Regulated Outer Membrane Receptor FauA in Alcaligin Siderophore-Mediated Iron Uptake in Bordetella Species
Phenotypic analysis using heterologous host systems localized putative Bordetella pertussis ferric alcaligin transport genes and Fur-binding sequences to a 3.8-kb genetic region downstream from the alcR regulator gene. Nucleotide sequencing identified a TonB-dependent receptor family homolog gene, fauA, predicted to encode a polypeptide with high amino acid sequence similarity with known bacterial ferric siderophore receptors. In Escherichia coli, the fauA genes of both B. pertussis and Bordetella bronchiseptica directed the production of a 79-kDa polypeptide, approximating the predicted size of the mature FauA protein. B. bronchiseptica fauA insertion mutant BRM17 was unable to utilize ferric alcaligin, and in complementation analyses ferric alcaligin utilization was restored to this mutant by supplying the wild-type fauA gene in trans. Mutant BRM18, carrying a nonpolar in-frame fauA deletion mutation, was defective in ferric alcaligin utilization and 55Fe-ferric alcaligin uptake and no longer produced a 79-kDa iron-regulated outer membrane protein. In complementation analyses, BRM18 merodiploids bearing the wild-type fauA gene in trans regained ferric alcaligin siderophore transport and utilization functions and produced the 79-kDa protein. Analysis of a plasmid-borne fauA-lacZ operon fusion confirmed that fauA is subject to iron regulation at the transcriptional level and that cis-acting transcriptional control elements mediating fauA iron repressibility reside within the 3.8-kb PstI fauA DNA region. Moreover, expression of the fauA-lacZ fusion gene under iron starvation conditions was shown to be alcR dependent. FauA is a 79-kDa iron-regulated outer membrane receptor protein required for transport and utilization of ferric alcaligin siderophore complexes by Bordetella species. Originally published Journal of Bacteriology, Vol. 181, No. 19, Oct 199
Zero-Point cooling and low heating of trapped 111Cd+ ions
We report on ground state laser cooling of single 111Cd+ ions confined in
radio-frequency (Paul) traps. Heating rates of trapped ion motion are measured
for two different trapping geometries and electrode materials, where no effort
was made to shield the electrodes from the atomic Cd source. The low measured
heating rates suggest that trapped 111Cd+ ions may be well-suited for
experiments involving quantum control of atomic motion, including applications
in quantum information science.Comment: 4 pages, 6 figures, Submitted to PR
The bloodstream differentiation - division of Trypanosoma brucei studied using mitochondrial markers
In the bloodstream of its mammalian host, the African trypanosome Trypanosoma brucei undergoes a life cycle stage differentiation from a long, slender form to a short, stumpy form. This involves three known major events: exit from a proliferative cell cycle, morphological change and mitochondrial biogenesis. Previously, models have been proposed accounting for these events (Matthews & Gull 1994a). Refinement of, and discrimination between, these models has been hindered by a lack of stage-regulated antigens useful as markers at the single-cell level. We have now evaluated a variety of cytological markers and applied them to investigate the coordination of phenotypic differentiation and cell cycle arrest. Our studies have focused on the differential expression of the mitochondrial enzyme dihydrolipoamide dehydrogenase relative to the differentiation-division of bloodstream trypanosomes. The results implicate a temporal order of events: commitment, division, phenotypic differentiation
Acts of Kindness and Acts of Novelty Affect Life Satisfaction
The present experiment was designed to establish the effects of acts of kindness and acts of novelty on life satisfaction. Participants aged 18–60 took part on a voluntary basis. They were randomly assigned to perform either acts of kindness, acts of novelty, or no acts on a daily basis for 10 days. Their life satisfaction was measured before and after the 10-day experiment. As expected, performing acts of kindness or acts of novelty resulted in an increase in life satisfaction
Magneto-optical Trapping of Cadmium
We report the laser-cooling and confinement of Cd atoms in a magneto-optical
trap, and characterize the loading process from the background Cd vapor. The
trapping laser drives the 1S0-1P1 transition at 229 nm in this two-electron
atom and also photoionizes atoms directly from the 1P1 state. This
photoionization overwhelms the other loss mechanisms and allows a direct
measurement of the photoionization cross section, which we measure to be
2(1)x10^(-16)cm^(2) from the 1P1 state. When combined with nearby laser-cooled
and trapped Cd^(+) ions, this apparatus could facilitate studies in ultracold
interactions between atoms and ions.Comment: 8 pages, 11 figure
Implementation of Grover's Quantum Search Algorithm in a Scalable System
We report the implementation of Grover's quantum search algorithm in the
scalable system of trapped atomic ion quantum bits. Any one of four possible
states of a two-qubit memory is marked, and following a single query of the
search space, the marked element is successfully recovered with an average
probability of 60(2)%. This exceeds the performance of any possible classical
search algorithm, which can only succeed with a maximum average probability of
50%.Comment: 4 pages, 3 figures, updated error discussio
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