Alcohol Impairs Bioenergetics and Differentiation Capacity of Myoblasts from Simian Immunodeficiency Virus-Infected Female Macaques

Supplemental Figure S1: Schematic diagram of oxygen consumption rate (OCR) trace for the Mito Stress Test and resulting parameters reported herein. Created using BioRender.com.Supplemental Figure S2. Schematic diagram of extracellular acidification rate (ECAR) trace for the Glycolysis Stress Test and resulting parameters reported herein. Created using BioRender.com.Supplemental Figure S3: Flow cytometry gating strategies. After gating for singlet events, total myoblasts were gated on a forward scatter (FSC)/side scatter (SS) plot and then gated for live myoblasts (APC-Cy7-A; A). Selected events were further gated for the subsets of interest, namely FITC-A for Mito Tracker Green (B), AmCyan-A for MitoSOX (C), co-expression of Mito Tracker Green and MitoSOX (D), and PE-A for TMRM (E).</p