Автоматизация планово-аналитической деятельности

Издание направлено на формирование и развитие у студентов нового типа экономического мышления, интереса и способностей к творческому решению задач, стоящих перед различными отраслями экономики на современном этапе с использованием новейших информационных технологий. Практикум предназначен для студентов специальности 1-25 01 07 "Экономика и управление на предприятии" специализаций 1-25 01 07 11 "Экономика и управление на предприятии промышленности", 1-25 01 07 20 "Экономика и управление на предприятии услуг"

Additional file 3: Table S2. of Emergency department hyperoxia is associated with increased mortality in mechanically ventilated patients: a cohort study

Multivariable logistic regression model with in-hospital mortality as the dependent variable. (DOCX 15 kb

sj-docx-1-jtt-10.1177_1357633X231221586 - Supplemental material for Pilot implementation of a telemedicine care bundle: Antimicrobial stewardship, patient satisfaction, clinician satisfaction, and usability in patients with sinusitis

Supplemental material, sj-docx-1-jtt-10.1177_1357633X231221586 for Pilot implementation of a telemedicine care bundle: Antimicrobial stewardship, patient satisfaction, clinician satisfaction, and usability in patients with sinusitis by Zoe Grabinski, Victoria Leybov, Sarah Battistich, Brian Roberts, Zachary Migliozzi, Yelan Wang, Harita Reddy, and Silas W Smith in Journal of Telemedicine and Telecare</p

Visualization 2: High-efficiency large-angle Pancharatnam phase deflector based on dual-twist design

-40 beam steering Originally published in Optics Express on 20 March 2017 (oe-25-6-6283

Godetia amoena Lilja

原著和名: イロマツヨヒ科名: アカバナ科 = Onagraceae採集地: 千葉県 四街道市 千代田 (下総 四街道市 千代田)採集日: 1985/5/27採集者: 萩庭丈壽整理番号: JH038808国立科学博物館整理番号: TNS-VS-98880

Additional file 1: Figures S1–S40. of Distinct gene regulatory programs define the inhibitory effects of liver X receptors and PPARG on cancer cell proliferation

All supplemental figures with captions. (PDF 23001 kb

Inhibition of wild type alphaviruses by Bortezomib.

<p>U87MG cells were pre-treated with Bortezomib at 0.1μM for 2 hours and then infected with VEEV TrD, EEEV strain GA97, and WEEV California 1930 strain at an MOI: 0.1 for 1 hour. Supernatants were collected 24 hours post infection and analyzed by plaque assay for infectious viral particles (PFU/mL). The arithmetic means are illustrated graphically. The graph is representative of 3 independent experiments where each experiment was performed in triplicate. Standard deviations were calculated accordingly. p<0.05(*).</p

Ubiquitinated capsid in virions.

<p>VEEV-containing supernatants obtained from infected VERO cells were subjected to sucrose density centrifugation as described in the materials and methods section. A) The collected fractions were analyzed by plaque assay and are represented graphically. Fraction 3 with the highest titer of virus was used for immunoprecipitation with capsid antibody and an isotype IgG as a control. Immunoprecipitated samples were resolved by SDS-PAGE and subsequently immunoblotted for ubiquitin (top panel) and capsid (bottom panel). The image is representative of duplicate immunoprecipitation runs.</p

Bortezomib decreases intracellular viral RNA.

<p>A) U87MG cells were seeded in a 96-well plate at a density of 10,000 cells per well. Cells were pre-treated with 0.1μM Bortezomib or DMSO for 2 hours and then infected with TC-83 (MOI: 0.1) for 1 hour. At 0, 2, 4, 6, and 8 hours post infection cells were lysed with MagMAX-96 Total RNA Isolation Kit. As controls, cells were either pre-treated with 0.1μM Bortezomib or DMSO for 2 hours, then infected with TC-83 (MOI: 0.1) and the cells lysed at 24 hours post infection. Levels of viral RNA were quantified by q-RT-PCR using VEEV specific primers. The cycling conditions and probe primer pair sequences are described in the methods section. The graphs represent an average of 2 independent experiments where each experiment was performed in triplicate. Standard deviations were calculated accordingly. p≤0.0001 (**). U87MG cells were seeded onto #1.0 coverslips in 24-well plates at a density of 54,000 cells per well. Cells were pretreated with Bortezomib for 2 hours and then infected with TC-83 (MOI: 5) (B and C). B) After 8 hours of infection, the cells were fixed and processed for RNA FISH as described in the materials and methods section. Following FISH staining, cells were imaged using a Zeiss 700 confocal microscope with a 20X objective and at least 10 high-powered fields (HPFs) were obtained for each sample. The percentage of infected to uninfected cells was calculated per HPF for each condition. A representative 20X objective image is shown with viral RNA in red and nuclei in blue. C) After 0, 4 and 8 hours of infection, the cells were fixed and processed for RNA FISH as described in the materials and methods section. Following FISH staining, cells were imaged using a Zeiss 700 confocal microscope. A representative 63X objective image is shown with viral RNA in red and nuclei in blue. D) VERO cells were infected with TC-83 or Rift Valley Fever Virus (RVFV) (strain MP-12) for 24 hours. Cells were then processed for RNA FISH and stained with VEEV vRNA specific FISH probes as described in the materials and methods section. Following the RNA FISH staining, the VEEV infected cells were immunostained with an anti- VEEV capsid antibody and the RVFV infected cells were immunostained with an anti-RVFV nucleocapsid antibody. Nuclei were detected using DAPI. Images are shown as condensed Z-stacks. The images are representative of 2 independent experiments performed in duplicate.</p

VEEV capsid protein is ubiquitinated on K48.

<p>A) U87MG cells were seeded in an 8-well chambered slide at 20,000 cells per well. The cells were uninfected (Mock) or infected with TC-83 at an MOI of 10. At 1, 2, 5 and 6 hours post infection, cells were fixed and processed as described in the materials and methods section. The cells were probed with K48 ubiquitin and capsid antibodies followed by incubation with Alexa-Fluor 488 and Alexa-Fluor 568 respectively. The cells were stained with DAPI to observe the nuclei. Images were taken using Nikon Eclipse TE2000-U with a 60X objective and are representative of 2 replicates in an experiment. Red boxes are parts of the image that have been zoomed in and displayed in Z-stacks. B) Number of infected U87MG cells showing co-localization tabulated. C) U87MG cells were seeded in an 8-well chambered slide at 20,000 cells per well. The cells were untreated (Mock), DMSO treated or Bortezomib treated (0.1μM) for 2 hours. The cells were uninfected (Mock) or infected with TC-83 at an MOI of 10. At 2 hours post infection, cells were fixed and processed as described in the materials and methods section. The cells were probed with K48 ubiquitin and capsid antibodies followed by incubation with Alexa-Fluor 488 and Alexa-Fluor 568 respectively. The cells were stained with DAPI to observe the nuclei. Images were taken using Nikon Eclipse TE2000-U with a 60X objective and are representative of 2 independent experiments performed in duplicate. D) Number of infected U87MG cells showing co-localization tabulated. E) U87MG cells were treated with Bortezomib (0.1μM) or DMSO for 2 hours and then infected with TC-83 (MOI: 5) for 1 hour. At 2 hours post infection cell lysates were collected, lysed and quantified. Equal amounts of total protein were immunoprecipitated with capsid antibody and resolved by SDS-PAGE and subsequently immunoblotted for K48 ubiquitin (top panel) and capsid (bottom panel). The image is representative of 2 independent experiments. Protein bands were quantified using Image J software and normalized to capsid. Average percent differences of 2 independent experiments are depicted graphically.</p