Estudio de factibilidad para la creación de un centro de entretenimiento innovador para los jóvenes de 15 a 24 años del Cantón Milagro.

Esta investigación se ha desarrollado con el objetivo de determinar la incidencia que tienen las actividades de entretenimiento actuales en la formación de malos hábitos en los jóvenes en el cantón Milagro. Para esto se desarrolló una debida investigación con aplicación de técnicas tales como la encuesta, la entrevista y observación que nos permitieron corroborar nuestras hipótesis. A través de dichas técnicas se obtuvieron resultados que nos permitieron determinar la relación que tiene la adquisición de malos hábitos en los jóvenes por las actividades de entretenimiento que realizan actualmente, Las cuales están dadas por las siguientes razones: El entorno social de los jóvenes, es decir, su círculo social de amigos y allegados. El consumo de productos y sustancias estupefacientes y psicotrópicas. La poca responsabilidad social de las empresas de entretenimiento por el bienestar personal de los jóvenes. La poca innovación que mantiene los modelos tradicionales de negocio en este sector. El presente proyecto se lo realiza para demostrar que existe un problema y a su vez con la finalidad de contribuir con una idea que permita mitigar el mismo, mediante la creación de un Centro de Entretenimiento libre de sustancias estupefacientes y psicotrópicas que tendrá como meta convertirse en la nueva forma y alternativa de diversión para los jóvenes. Esta propuesta surge con el objeto de demostrar que una empresa puede ser rentable y a su vez tener un impacto positivo en la sociedad, específicamente en los jóvenes del cantón Milagro, ya que es en estas edades que se es más vulnerable ante el consumo de alcohol, tabaco y cualquier tipo de drogas. Con esta propuesta se fomenta una nueva forma de diversión a través de actividades que no inciten al consumo de este tipo de sustancias perjudiciales

TRY plant trait database – enhanced coverage and open access

Plant traits - the morphological, anatomical, physiological, biochemical and phenological characteristics of plants - determine how plants respond to environmental factors, affect other trophic levels, and influence ecosystem properties and their benefits and detriments to people. Plant trait data thus represent the basis for a vast area of research spanning from evolutionary biology, community and functional ecology, to biodiversity conservation, ecosystem and landscape management, restoration, biogeography and earth system modelling. Since its foundation in 2007, the TRY database of plant traits has grown continuously. It now provides unprecedented data coverage under an open access data policy and is the main plant trait database used by the research community worldwide. Increasingly, the TRY database also supports new frontiers of trait‐based plant research, including the identification of data gaps and the subsequent mobilization or measurement of new data. To support this development, in this article we evaluate the extent of the trait data compiled in TRY and analyse emerging patterns of data coverage and representativeness. Best species coverage is achieved for categorical traits - almost complete coverage for ‘plant growth form’. However, most traits relevant for ecology and vegetation modelling are characterized by continuous intraspecific variation and trait–environmental relationships. These traits have to be measured on individual plants in their respective environment. Despite unprecedented data coverage, we observe a humbling lack of completeness and representativeness of these continuous traits in many aspects. We, therefore, conclude that reducing data gaps and biases in the TRY database remains a key challenge and requires a coordinated approach to data mobilization and trait measurements. This can only be achieved in collaboration with other initiatives

Ultralight vector dark matter search using data from the KAGRA O3GK run

Among the various candidates for dark matter (DM), ultralight vector DM can be probed by laser interferometric gravitational wave detectors through the measurement of oscillating length changes in the arm cavities. In this context, KAGRA has a unique feature due to differing compositions of its mirrors, enhancing the signal of vector DM in the length change in the auxiliary channels. Here we present the result of a search for U(1)B−L gauge boson DM using the KAGRA data from auxiliary length channels during the first joint observation run together with GEO600. By applying our search pipeline, which takes into account the stochastic nature of ultralight DM, upper bounds on the coupling strength between the U(1)B−L gauge boson and ordinary matter are obtained for a range of DM masses. While our constraints are less stringent than those derived from previous experiments, this study demonstrates the applicability of our method to the lower-mass vector DM search, which is made difficult in this measurement by the short observation time compared to the auto-correlation time scale of DM

Two cases of severe pulmonary toxicity from highly active mesothelin-directed CAR T cells.

Multiple clinical studies have treated mesothelin (MSLN)-positive solid tumors by administering MSLN-directed chimeric antigen receptor (CAR) T cells. Although these products are generally safe, efficacy is limited. Therefore, we generated and characterized a potent, fully human anti-MSLN CAR. In a phase 1 dose-escalation study of patients with solid tumors, we observed two cases of severe pulmonary toxicity following intravenous infusion of this product in the high-dose cohort (1-3 × 108 T cells per m2). Both patients demonstrated progressive hypoxemia within 48 h of infusion with clinical and laboratory findings consistent with cytokine release syndrome. One patient ultimately progressed to grade 5 respiratory failure. An autopsy revealed acute lung injury, extensive T cell infiltration, and accumulation of CAR T cells in the lungs. RNA and protein detection techniques confirmed low levels of MSLN expression by benign pulmonary epithelial cells in affected lung and lung samples obtained from other inflammatory or fibrotic conditions, indicating that pulmonary pneumocyte and not pleural expression of mesothelin may lead to dose-limiting toxicity. We suggest patient enrollment criteria and dosing regimens of MSLN-directed therapies consider the possibility of dynamic expression of mesothelin in benign lung with a special concern for patients with underlying inflammatory or fibrotic conditions

Methylation of p53 by Set7/9 mediates p53 acetylation and activity in vivo.

The protein methyltransferase Set7/9 was recently shown to regulate p53 activity in cancer cells. However, the impact of Set7/9 on p53 function in vivo is unclear. To explore these issues, we created a null allele of Set7/9 in mice. Cells from Set7/9 mutant mice fail to methylate p53 K369, are unable to induce p53 downstream targets upon DNA damage, and are predisposed to oncogenic transformation. Importantly, we find that methylation of p53 by Set7/9 is required for the binding of the acetyltransferase Tip60 to p53 and for the subsequent acetylation of p53. We provide the first genetic evidence demonstrating that lysine methylation of p53 by Set7/9 is important for p53 activation in vivo and suggest a mechanistic link between methylation and acetylation of p53 through Tip60