Micrurus limbatus

Number of Pages: 3Integrative BiologyGeological Science

Development of quantum perspectives in modern physics

Introductory undergraduate courses in classical physics stress a perspective that can be characterized as realist; from this perspective, all physical properties of a classical system can be simultaneously specified and thus determined at all future times. Such a perspective can be problematic for introductory quantum physics students, who must develop new perspectives in order to properly interpret what it means to have knowledge of quantum systems. We document this evolution in student thinking in part through pre- and post-instruction evaluations using the Colorado Learning Attitudes about Science Survey. We further characterize variations in student epistemic and ontological commitments by examining responses to two essay questions, coupled with responses to supplemental quantum attitude statements. We find that, after instruction in modern physics, many students are still exhibiting a realist perspective in contexts where a quantum-mechanical perspective is needed. We further find that this effect can be significantly influenced by instruction, where we observe variations for courses with differing learning goals. We also note that students generally do not employ either a realist or a quantum perspective in a consistent manner.Comment: 18 pages, plus references; 3 figures; 9 tables. PACS: 01.40.Fk, 03.65._

ZoomQuant: An application for the quantitation of stable isotope labeled peptides

The main goal of comparative proteomics is the quantitation of the differences in abundance of many proteins between two different biological samples in a single experiment. By differentially labeling the peptides from the two samples and combining them in a single analysis, relative ratios of protein abundance can be accurately determined. Protease catalyzed 18O exchange is a simple method to differentially label peptides, but the lack of robust software tools to analyze the data from mass spectra of 18O labeled peptides generated by common ion trap mass spectrometers has been a limitation. ZoomQuant is a stand-alone computational tool that analyzes the mass spectra of 18O labeled peptides from ion trap instruments and determines relative abundance ratios between two samples. Starting with a filtered list of candidate peptides that have been successfully identified by Sequest, ZoomQuant analyzes the isotopic forms of the peptides using high-resolution zoom scan spectrum data. The theoretical isotope distribution is determined from the peptide sequence and is used to deconvolute the peak areas associated with the unlabeled, partially labeled, and fully labeled species. The ratio between the labeled and unlabeled peptides is then calculated using several different methods. ZoomQuant’s graphical user interface allows the user to view and adjust the parameters for peak calling and quantitation and select which peptides should contribute to the overall abundance ratio calculation. Finally, ZoomQuant generates a summary report of the relative abundance of the peptides identified in the two samples

The Dynamic Universe: Realizing the Potential of Classical Time Domain and Multimessenger Astrophysics

In parallel with the multi-messenger revolution, major advances in time-domain astronomy across multiple science disciplines relevant to astrophysics are becoming more urgent to address. Aside from electromagnetic observations of gravitational wave events and explosive counterparts, there are a number of “classical” astrophysical areas that require new thinking for proper exploration in the time domain. How NASA, NSF, ESA, and ESO consider the 2020 USA Decadal Survey within the astronomy community, as well as the worldwide call to support and expand time domain and multi-messenger astrophysics, it is crucial that all areas of astrophysics, including stellar, galactic, Solar System, and exoplanetary science participate in the discussion, and that it not be made into an exclusive preserve of cosmological, high-energy, explosive and transient science. Time domain astronomy is used to explore many aspects of astrophysics–particularly concerning ground- and space-based mission science goals of exploring how the Universe works, understanding how did we get here, and are we alone. Time domain studies are already built into the core operations of many currently operating and future space telescopes (e.g., Roman, PLATO) as well as current and planned large areal ground-based surveys (e.g., Rubin). Time-domain observations designed for one scientific purpose, also lead to great discoveries in many other science areas. The recent advent of user-friendly hardware, software, observational approaches, and online data infrastructure has also helped make time domain observations especially suitable and appealing for citizen science projects. We provide a review of the current state of TDAMM alerts and observational protocols, revealing a wide array of software and applications, much of which is incompatible. Any conversation regarding TDAMM astrophysics should include all aspects of the field, including those aspects seen as classical applications

In vivo monitoring of fetoplacental Vegfr2 gene activity in a murine pregnancy model using a Vegfr2-luc reporter gene and bioluminescent imaging

<p>Abstract</p> <p>Background</p> <p>Vascular endothelial growth factor receptor-2 (VEGFR2) plays a pivotal role in angiogenesis by eliciting vascular endothelial cell growth when bound to VEGF, a powerful pro-angiogenic ligand. While Vegf and Vegfr2 are expressed throughout gestation, the latter third of gestation in mice is characterized by a marked increase in fetoplacental angiogenesis. Thus, the objective of this study was to determine the feasibility of monitoring fetoplacental Vegfr2 gene activity non-invasively using a Vegfr2-luc reporter transgenic mouse and bioluminescent imaging.</p> <p>Methods</p> <p>Imaging parameters were optimized using two wild-type (WT) females, bearing Vegfr2-luc fetuses. Then, seven WT females, bred to Vegfr2-luc males, were imaged from gestational day (GD) 12 to 18 to determine the usefulness of the Vegfr2-luc mouse as a model for studying fetoplacental Vegfr2 activity during pregnancy. Semi-quantitative RT-PCR of Vegfr2 was also performed on whole fetoplacental units during this time. Additionally, resultant neonates were imaged at postnatal day (PND) 7, 14 and 21 to monitor Vegfr2 activity during post-natal development.</p> <p>Results</p> <p>Fetoplacental Vegfr2 gene activity was detected as light emissions beginning on GD 12 of gestation and increased throughout the imaging period (P < 0.05), and this paralleled the Vegfr2 mRNA data obtained from RT-PCR analysis. A decline in fetoplacental light emissions was associated with a poor pregnancy outcome in one pregnancy, indicating that this approach has potential use for studies monitoring pregnancy well being. Additionally, neonatal Vegfr2 activity was detected at PND 7, 14 and 21 but declined with time (P < 0.0001).</p> <p>Conclusions</p> <p><it>In utero </it>fetoplacental Vegfr2 gene activity was monitored longitudinally in a quantitative manner using a luciferase reporter gene and bioluminescent imaging during the latter third of gestation. This study demonstrates the feasibility of using the Vegfr2-luc mouse to monitor late gestation fetoplacental angiogenic activity under normal and experimental conditions. Additionally, neonatal Vegfr2 gene activity was monitored for three weeks postpartum, allowing continuous monitoring of Vegfr2 activity during the latter third of gestation and postnatal development within the same animals.</p

Cosmological String Gas on Orbifolds

It has long been known that strings wound around incontractible cycles can play a vital role in cosmology. In particular, in a spacetime with toroidal spatial hypersurfaces, the dynamics of the winding modes may help yield three large spatial dimensions. However, toroidal compactifications are phenomenologically unrealistic. In this paper we therefore take a first step toward extending these cosmological considerations to $D$-dimensional toroidal orbifolds. We use numerical simulation to study the timescales over which "pseudo-wound" strings unwind on these orbifolds with trivial fundamental group. We show that pseudo-wound strings can persist for many ``Hubble times'' in some of these spaces, suggesting that they may affect the dynamics in the same way as genuinely wound strings. We also outline some possible extensions that include higher-dimensional wrapped branes.Comment: 14 pages, 8 eps fig

Prognathodes geminus, a new species of butterflyfish (Teleostei, Chaetodontidae) from Palau

A new species of the butterflyfish genus Prognathodes (Chaetodontidae) is described from two specimens collected at a depth of 116 m off Ngemelis Island, Palau. Prognathodes geminus sp. n. is similar to P. basabei Pyle &amp;amp; Kosaki, 2016 from the Hawaiian archipelago, and P. guezei (Maugé &amp;amp; Bauchot, 1976) from the western Indian Ocean, but differs from these species in the number of soft dorsal-fin rays, size of head, body width, and body depth. There are also subtle differences in life color, and substantial differences in the mtDNA cytochrome oxidase I sequence (d ≈ 0.08). Although genetic comparisons with P. guezei are unavailable, it is expected that the genetic divergence between P. guezei and P. geminus will be even greater than that between P. geminus and P. basabei. It is named for the strikingly similar color pattern it shares with P. basabei