8 research outputs found
Molecular characterisation of Cryptosporidium spp. in lambs in the South Central region of the State of São Paulo
Soroepidemiologia de Toxoplasma gondii em gatos domiciliados atendidos em clínicas particulares de Porto Alegre, RS, Brasil
A New Laboratorial Method For The Diagnosis Of Gastrointestinal Parasites In Dogs [um Novo Método Laboratorial Para Diagnóstico De Parasitos Gastrointestinais Em Cães]
In this study, we aimed to introduce a new technique called TF-Test Modified/Dog for the diagnosis of gastrointestinal parasites in dogs. Fecal samples from 106 dogs were processed by the technique TF-Test Modified/Dog and the techniques of centrifugation-flotation in zinc sulfate, simple-flotation by saturated solution of sodium chloride, direct microscopy exam and TF-Test Conventional. Sensitivity was higher in the TF-Test Modified/Dog (98.41%), followed by flotation in saturated zinc sulfate (77.78%), TF-Test Conventional (73.02%), flotation by saturated sodium chloride (55.55%), and direct microscopy exam (30.16%). The diagnostic efficiency varied from 58.49% to 99.06%, with the highest value also obtained by the new proposed technique. Efficiency level of 99.06% with kappa index 0.979 (almost perfect) was obtained with the TF-Test Modified/Dog. These results represent significant statistical gains (P < 0.05) of 20.63% in sensitivity and 12.27% in efficiency over the best among the other techniques - flotation by saturated zinc sulfate, whose kappa index was 0.738, much lower than that of the TF-Test Modified/Dog. All techniques presented 100% specificity. In this sense, the high sensitivity of the TF-Test Modified/Dog makes it suitable for epidemiological surveys of gastrointestinal parasitosis in dogs, zoonoses control and preventive surveillance programs.22115Bridger, K.E., Whitney, H., Gastrointestinal parasites in dogs from the Island of St. Pierre off the south coast of Newfoundland (2009) Vet Parasitol, 162 (1), pp. 167-170. , http://dx.doi.org/10.1016/j.vetpar.2009.02.016Falcão, A.X., Gomes, J.F., Hoshino-Shimizu, S., Suzuki, C.T.N., (2010) Método de preparação de amostra coproparasitológica fecal e composição clarificante, , http://www.wipo.int/pctdb, (Concessão de patente PTC: Prot. 018100037856) [cited 2010 Mai 25]. Avaliable fromFaust, E.C., D'Antoni, J.S., Odom, V., Miller, M.J., Peres, C., Sawitz, W., A critical study of clinical laboratory technics for the diagnosis of protozoan cysts and helminth eggs in feces. I. 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Washington: ASMGennari, S.M., Kasai, N., Cortez, A., Ocorrência de protozoários e helmintos em amostras de fezes de cães e gatos da cidade de São Paulo (2001) Braz J Vet Res Anim Sci, 36 (2), pp. 87-91Gomes, J.F., Lumina, G., Amarante, A.F.T., Hoshino-Shimizu, S., Leme, D.P., Dias, L.C.S., Using the TF-Test Kit for the parasitological Diagnosis in dogs (Canis familiaris) (2006) Braz J Parasitol Vet, p. 406. , 001-043Gomes, J.F., Hoshino-Shimizu, S., Dias, L.C., Araujo, A.J., Castilho, V.L., Neves, F.A., Evaluation of a novel Kit (TF-Test) for the diagnosis of intestinal parasitic infections (2004) J Clin Lab Anal, 18 (2), pp. 132-138. , http://dx.doi.org/10.1002/jcla.20011Hendrix, C.M., Robinson, E., (2006) Diagnostic Parasitology for Veterinary Technicians, , 3rd ed. MosbyHoshino-Shimizu, S., Gomes, J.F., Dias, L.C.S., Intestinal Parasites: Traditional techniques and commercial kits (2001) Braz J Parasitol, 37, p. 44Katagiri, S., Oliveira-Sequeira, T.C.G., Comparison of three concentration methods for the recovery of canine intestinal parasites from stool samples (2010) Exp Parasitol, 126 (2), pp. 214-216. , http://dx.doi.org/10.1016/j.exppara.2010.04.027Katagiri, S., Oliveira-Sequeira, T.C.G., Prevalence of Dog Intestinal Parasites and Risk Perception of Zoonotic Infection by Dog Owners in São Paulo State, Brazil (2008) Zoonoses Public Health, 55 (8-10), pp. 406-413. , http://dx.doi.org/10.1111/j.1863-2378.2008.01163.xKato, K., Miura, M., Comparative examinations (1954) Jpn J Parasitol, 3, p. 35Lumina, G., Bricarello, P.A., Gomes, J.F., Amarante, A.F.T., The evaluation of TF-Test Kit for diagnosis of gastrointestinal parasite infections in sheep (2006) Braz J Vet Res Anim Sci, 43, pp. 496-501McLure, M., Willet, W.C., Misinterpretation and misuse of the Kappa statistic (1987) Am J Epidemiol, 126 (2), pp. 161-169. , http://dx.doi.org/10.1093/aje/126.2.161McCarthy, J., Moore, T.A., Emerging helminth zoonoses (2000) Int J Parasitol, 30 (12-13), pp. 1351-1360. , http://dx.doi.org/10.1016/S0020-7519(00)00122-3Martínez-Carrasco, C., Berriatua, E., Garijo, M., Martínez, J., Alonso, F.D., Ruiz de Ybáñez, R., Epidemiological study of non-systemic parasitism in dogs in southeast mediterranean Spain assessed by coprological and post-mortem examination (2007) Zoonoses Public Health, 54 (5), pp. 195-203. , http://dx.doi.org/10.1111/j.1863-2378.2007.01047.xMeireles, M.V., Cryptosporidium infection in Brazil: Implications for veterinary medicine and public health (2010) Rev Bras Parasitol Vet, 19 (4), pp. 197-204. , http://dx.doi.org/10.1590/S1984-29612010000400002Oliveira-Sequeira, T.C.G., Amarante, A.F.T., Ferrari, T.B., Nunes, L.C., Prevalence of intestinal parasites in dogs from São Paulo State, Brazil (2002) Vet Parasitol, 103 (1-2), pp. 19-27. , http://dx.doi.org/10.1016/S0304-4017(01)00575-1Rodie, G., Stafford, P., Holland, C., Wolfe, A., Contamination of dog hair with eggs of Toxocara canis (2008) Vet Parasitol, 152 (1-2), pp. 85-93. , http://dx.doi.org/10.1016/j.vetpar.2007.12.008Sánchez-Thevenet, P., Jensen, O., Mellado, I., Torrecillas, C., Raso, S., Flores, M.E., Presence and persistence of intestinal parasites in canine fecal material collected from the environment in the Province of Chubut, Argentine Patagonia (2003) Vet Parasitol, 117 (4), pp. 263-269. , http://dx.doi.org/10.1016/j.vetpar.2003.09.014Soriano, S.V., Pierangeli, N.B., Roccia, I., Bergagna, H.F.J., Lazzarini, L.E., Celescinco, A., A wide diversity of zoonotic intestinal parasites infects urban and rural dogs in Neuquén, Patagonia, Argentina (2009) Vet Parasitol, 167 (1), pp. 81-85. , http://dx.doi.org/10.1016/j.vetpar.2009.09.048Sousa, V.R., Almeida, A.F., Cândido, A.C., Barros, L.A., Ovos e larvas de helmintos em caixas de areia de creches, escolas municipais e praças públicas de Cuiabá, MT (2010) Cienc Anim Bras, 11 (2), pp. 390-395. , http://dx.doi.org/10.5216/cab.v11i2.3150Táparo, C.V., Perri, S.H., Serrano, A.C., Ishizaki, M.N., da Costa, T.P., Amarante, A.F.T., Comparison between coproparasitological techniques for the diagnosis of helminth eggs or protozoa oocysts in dogs (2006) Rev Bras Parasitol Vet, 15 (1), pp. 1-5Ueno, H., Gonçalves, P.C., (1998) Manual for diagnosis of helminthiasis in ruminants, , 4rd ed. Tokyo: J. I. C. AWillis, H.H., A simple levitation method for the detection of hookworm ova (1921) Med J Aust, 29, pp. 375-376Xiao, L., Cama, A.V., Cabrera, L., Ortega, Y., Pearson, J., Gilman, R.H., Possible transmission of Cryptosporidium canis among children and a dog in a household (2007) J Clin Microbiol, 45 (6), pp. 2014-2016. , http://dx.doi.org/10.1128/JCM.00503-0
Validation Of A New Technique To Detect Cryptosporidium Spp. Oocysts In Bovine Feces
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Due to its important zoonotic potential, cryptosporidiosis arouses strong interest in the scientific community, because, it was initially considered a rare and opportunistic disease. The parasitological diagnosis of the causative agent of this disease, the protozoan Cryptosporidium spp., requires the use of specific techniques of concentration and permanent staining, which are laborious and costly, and are difficult to use in routine laboratory tests. In view of the above, we conducted the feasibility, development, evaluation and intralaboratory validation of a new parasitological technique for analysis in optical microscopy of Cryptosporidium spp. oocysts, called TF-Test Coccidia, using fecal samples from calves from the city of Araçatuba, São Paulo. To confirm the aforementioned parasite and prove the diagnostic efficiency of the new technique, we used two established methodologies in the scientific literature: parasite concentration by centrifugal sedimentation and negative staining with malachite green (CSN-Malachite) and Nested-PCR. We observed good effectiveness of the TF-Test Coccidia technique, being statistically equivalent to CSN-Malachite. Thus, we verified the effectiveness of the TF-Test Coccidia parasitological technique for the detection of Cryptosporidium spp. oocysts and observed good concentration and morphology of the parasite, with a low amount of debris in the fecal smear. © 2016 Elsevier B.V.13415#99/06228-4, FAPESP, São Paulo Research FoundationFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP
Validation of a new technique to detect Cryptosporidium spp. oocysts in bovine feces
Due to its important zoonotic potential, cryptosporidiosis arouses strong interest in the scientific community, because, it was initially considered a rare and opportunistic disease. The parasitological diagnosis of the causative agent of this disease, the protozoan Cryptosporidium spp., requires the use of specific techniques of concentration and permanent staining, which are laborious and costly, and are difficult to use in routine laboratory tests. In view of the above, we conducted the feasibility, development, evaluation and intralaboratory validation of a new parasitological technique for analysis in optical microscopy of Cryptosporidium spp. oocysts, called TF-Test Coccidia, using fecal samples from calves from the city of Araçatuba, São Paulo. To confirm the aforementioned parasite and prove the diagnostic efficiency of the new technique, we used two established methodologies in the scientific literature: parasite concentration by centrifugal sedimentation and negative staining with malachite green (CSN-Malachite) and Nested-PCR. We observed good effectiveness of the TF-Test Coccidia technique, being statistically equivalent to CSN-Malachite. Thus, we verified the effectiveness of the TF-Test Coccidia parasitological technique for the detection of Cryptosporidium spp. oocysts and observed good concentration and morphology of the parasite, with a low amount of debris in the fecal smear.13415COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPESFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESPSem informação99/06228-