Mixed plastics waste valorization through tandem chemical oxidation and biological funneling

115 p.-4 fig.-45 fig. supl.-14 tab supl.Mixed plastics waste represents an abundant and largely untapped feedstock for the production of valuable products. The chemical diversity and complexity of thesematerials, however, present major barriers to realizing this opportunity. In this work, we show that metal-catalyzed autoxidation depolymerizes comingled polymers into a mixture of oxygenated small molecules that are advantaged substrates for biological conversion. We engineer a robust soil bacterium, Pseudomonas putida, to funnel these oxygenated compounds into a single exemplary chemical product, either b-ketoadipate or polyhydroxyalkanoates. This hybrid process establishes a strategy for the selective conversion of mixed plastics waste into useful chemical products.Funding was provided by the US Department of Energy, Office of Energy Efficiency and Renewable Energy, Advanced Manufacturing Office (AMO), and Bioenergy Technologies Office (BETO). This work was performed as part of the BOTTLE Consortium and was supported by AMO and BETO under contract no. DE-AC36- 08GO28308 with the National Renewable Energy Laboratory (NREL),operated by the Alliance for Sustainable Energy, LLC. The BOTTLE Consortium includes members from MIT, funded under contract no. DE-AC36-08GO28308 with NREL. Contributions by S.S.S. were supported by the US Department of Energy, Office of Basic Energy Sciences, under award no. DEFG02-05ER15690.Peer reviewe

Length-Weight Relationships, Age and Growth, and Body Condition of the Spottail Shiner (Notropis hudsonius) (Clinton 1824) in the Western Basin of Lake Erie

Total length (LT) and standard length (LS)(mm) were compared to weight MB(g) in the Spottail Shiner, Notropis hudsonius (Clinton 1824), in the Western Basin of Lake Erie.  Length and weight relationship (n = 529), length-frequency distribution, and sex ratio were evaluated for tributary habitats and compared to coastal habitats.  The total population had a positive correlation between log normalized LT and MB (y = 3.0902x-5.2289, R2= 0.995) and a positive correlation between LS and MB (y=3.1397x-5.0501, R2= 0.996). Male Spottail Shiner had a positive correlation between log normalized LT and MB (y = 3.0984x-5.2465, R2=0.995,) and a significant positive correlation between log normalized standard length and body weight (y = 3.1551x-5.0775, R2=0.996). Female Spottail Shiner had a positive correlation between log normalized LT and MB (y = 3.078x-5.2034, R2=0.993) and a positive correlation between log normalized LS and MB (y = 3.1338x-5.0393, R2=0.996). Males and females were not significantly different in LT or LS (F-test = 1.020, df = 260, 267, p = 0.437). Sex ratio was 1:1. Spottail Shiner exhibit indeterminate growth and did not exhibit gender influenced growth patterns. Tributary individuals were significantly smaller than coastal individuals (F-test = 0.346, df = 65, 202, p = < 0.001). Male age I individuals ranged from 12-54 mm, age II individuals ranged from 57-99 mm, and age III individuals ranged from 99-132 mm.  Female Age I individuals ranged from 12-48 mm, age II individuals ranged from 53-102 mm, and age III individuals ranged from 102-129 mm.