Mode spectrum and temporal soliton formation in optical microresonators

The formation of temporal dissipative solitons in optical microresonators enables compact, high repetition rate sources of ultra-short pulses as well as low noise, broadband optical frequency combs with smooth spectral envelopes. Here we study the influence of the resonator mode spectrum on temporal soliton formation. Using frequency comb assisted diode laser spectroscopy, the measured mode structure of crystalline MgF2 resonators are correlated with temporal soliton formation. While an overal general anomalous dispersion is required, it is found that higher order dispersion can be tolerated as long as it does not dominate the resonator's mode structure. Mode coupling induced avoided crossings in the resonator mode spectrum are found to prevent soliton formation, when affecting resonator modes close to the pump laser. The experimental observations are in excellent agreement with numerical simulations based on the nonlinear coupled mode equations, which reveal the rich interplay of mode crossings and soliton formation

Relative size selection of a conjugated polyelectrolyte in virus-like protein structures

A conjugated polyelectrolyte poly[(2-methoxy-5-propyloxy sulfonate)-phenyl-ene vinylene] (MPS-PPV) drives the assembly of virus capsid proteins to form single virus-like particles (VLPs) and aggregates with more than two VLPs, with a relative selection of high molecular weight polymer in the latter

Temporal solitons in optical microresonators

Dissipative solitons can emerge in a wide variety of dissipative nonlinear systems throughout the fields of optics, medicine or biology. Dissipative solitons can also exist in Kerr-nonlinear optical resonators and rely on the double balance between parametric gain and resonator loss on the one hand and nonlinearity and diffraction or dispersion on the other hand. Mathematically these solitons are solution to the Lugiato-Lefever equation and exist on top of a continuous wave (cw) background. Here we report the observation of temporal dissipative solitons in a high-Q optical microresonator. The solitons are spontaneously generated when the pump laser is tuned through the effective zero detuning point of a high-Q resonance, leading to an effective red-detuned pumping. Red-detuned pumping marks a fundamentally new operating regime in nonlinear microresonators. While usually unstablethis regime acquires unique stability in the presence of solitons without any active feedback on the system. The number of solitons in the resonator can be controlled via the pump laser detuning and transitions to and between soliton states are associated with discontinuous steps in the resonator transmission. Beyond enabling to study soliton physics such as soliton crystals our observations open the route towards compact, high repetition-rate femto-second sources, where the operating wavelength is not bound to the availability of broadband laser gain media. The single soliton states correspond in the frequency domain to low-noise optical frequency combs with smooth spectral envelopes, critical to applications in broadband spectroscopy, telecommunications, astronomy and low phase-noise microwave generation.Comment: Includes Supplementary Informatio

Quantum dot encapsulation in virus-like particles with tuneable structural properties and low toxicity

A simple method for the encapsulation of quantum dots (QDs) in virus-like particle (VLP) nanoassemblies with tuneable structural properties and enhanced biocompatibility is presented. Cowpea chlorotic mottle virus-based capsid proteins assemble around the carboxylated QDs to form QD/VLP nanoassemblies of different capsid size as a function of pH and ionic strength. Detailed structural characterizations verify that nanoassemblies with probably native capsid icosahedral symmetry (T = 3) are obtained at low pH and high ionic strength (pH 5.0, 1.0 M NaCl), whereas high pH and low ionic strength conditions (pH 7.5, 0.3 M NaCl) result in the formation of smaller assembly sizes similar to T = 1 symmetry. In vitro studies reveal that QD/VLP nanoassemblies are efficiently internalized by RAW 264.7 macrophages and HeLa cells with no signs of toxicity at QD concentrations exceeding the potentially-toxic levels. The presented route holds great promise for preparation of size-tuneable, robust, non-toxic luminescent probes for long term cellular imaging applications. Furthermore, thanks to the possibility of chemical and genetic manipulation of the viral protein shell encaging the QDs, the nanoassemblies have potential for in vivo targeting applications

Expression profiles of hydrophobic surfactant proteins in children with diffuse chronic lung disease

BACKGROUND: Abnormalities of the intracellular metabolism of the hydrophobic surfactant proteins SP-B and SP-C and their precursors may be causally linked to chronic childhood diffuse lung diseases. The profile of these proteins in the alveolar space is unknown in such subjects. METHODS: We analyzed bronchoalveolar lavage fluid by Western blotting for SP-B, SP-C and their proforms in children with pulmonary alveolar proteinosis (PAP, n = 15), children with no SP-B (n = 6), children with chronic respiratory distress of unknown cause (cRD, n = 7), in comparison to children without lung disease (n = 15) or chronic obstructive bronchitis (n = 19). RESULTS: Pro-SP-B of 25–26 kD was commonly abundant in all groups of subjects, suggesting that their presence is not of diagnostic value for processing defects. In contrast, pro-SP-B peptides cleaved off during intracellular processing of SP-B and smaller than 19–21 kD, were exclusively found in PAP and cRD. In 4 of 6 children with no SP-B, mutations of SFTPB or SPTPC genes were found. Pro-SP-C forms were identified at very low frequency. Their presence was clearly, but not exclusively associated with mutations of the SFTPB and SPTPC genes, impeding their usage as candidates for diagnostic screening. CONCLUSION: Immuno-analysis of the hydrophobic surfactant proteins and their precursor forms in bronchoalveolar lavage is minimally invasive and can give valuable clues for the involvement of processing abnormalities in pediatric pulmonary disorders

A Kerr Polarization Controller

Kerr-effect-induced changes of the polarization state of light are well known in pulsed laser systems. An example is nonlinear polarization rotation, which is critical to the operation of many types of mode-locked lasers. Here, we demonstrate that the Kerr effect in a high-finesse Fabry-Pérot resonator can be utilized to control the polarization of a continuous wave laser. It is shown that a linearly-polarized input field is converted into a left- or right-circularly-polarized field, controlled via the optical power. The observations are explained by Kerr-nonlinearity induced symmetry breaking, which splits the resonance frequencies of degenerate modes with opposite polarization handedness in an otherwise symmetric resonator. The all-optical polarization control is demonstrated at threshold powers down to 7 mW. The physical principle of such Kerr effect-based polarization controllers is generic to high-Q Kerr-nonlinear resonators and could also be implemented in photonic integrated circuits. Beyond polarization control, the spontaneous symmetry breaking of polarization states could be used for polarization filters or highly sensitive polarization sensors when operated close to the symmetry-breaking point

Hermansky-Pudlak syndrome type 2 manifests with fibrosing lung disease early in childhood

Background: Hermansky-Pudlak syndrome (HPS), a hereditary multisystem disorder with oculocutaneous albinism, may be caused by mutations in one of at least 10 separate genes. The HPS-2 subtype is distinguished by the presence of neutropenia and knowledge of its pulmonary phenotype in children is scarce. Methods: Six children with genetically proven HPS-2 presented to the chILD-EU register between 2009 and 2017; the data were collected systematically and imaging studies were scored blinded. Results: Pulmonary symptoms including dyspnea, coughing, need for oxygen, and clubbing started 3.3 years before the diagnosis was made at the mean age of 8.83 years (range 2-15). All children had recurrent pulmonary infections, 3 had a spontaneous pneumothorax, and 4 developed scoliosis. The frequency of pulmonary complaints increased over time. The leading radiographic pattern was ground-glass opacities with a rapid increase in reticular pattern and traction bronchiectasis between initial and follow-up Computer tomography (CT) in all subjects. Honeycombing and cysts were newly detectable in 3 patients. Half of the patients received a lung biopsy for diagnosis; histological patterns were cellular non-specific interstitial pneumonia, usual interstitial pneumonia-like, and desquamative interstitial pneumonia. Conclusions: HPS-2 is characterized by a rapidly fibrosing lung disease during early childhood. Effective treatments are required