30 research outputs found
The seasonal cycle of submesoscale flows
The seasonal cycle of submesoscale flows in the upper ocean is investigated in an idealised model domain analogous to mid-latitude open ocean regions. Submesoscale processes become much stronger as the resolution is increased, though with limited evidence for convergence of the solutions. Frontogenetical processes increase horizontal buoyancy gradients when the mixed layer is shallow in summer, while overturning instabilities weaken the horizontal buoyancy gradients as the mixed layer deepens in winter. The horizontal wavenumber spectral slopes of surface temperature and velocity are steep in summer and then shallow in winter. This is consistent with stronger mixed layer instabilities developing as the mixed layer deepens and energising the submesoscale. The degree of geostrophic balance falls as the resolution is made finer, with evidence for stronger non-linear and high-frequency processes becoming more important as the mixed layer deepens. Ekman buoyancy fluxes can be much stronger than surface cooling and are locally dominant in setting the stratification and the potential vorticity at fronts, particularly in the early winter. Up to 30% of the mixed layer volume in winter has negative potential vorticity and symmetric instability is predicted inside mesoscale eddies as well as in the frontal regions outside of the vortices
Direct binding of phosphatidylglycerol at specific sites modulates desensitization of a ligand-gated ion channel
Pentameric ligand-gated ion channels (pLGICs) are essential determinants of synaptic transmission, and are modulated by specific lipids including anionic phospholipids. The exact modulatory effect of anionic phospholipids in pLGICs and the mechanism of this effect are not well understood. Using native mass spectrometry, coarse-grained molecular dynamics simulations and functional assays, we show that the anionic phospholipid, 1-palmitoyl-2-oleoyl phosphatidylglycerol (POPG), preferentially binds to and stabilizes the pLGIC, Erwinia ligand-gated ion channel (ELIC), and decreases ELIC desensitization. Mutations of five arginines located in the interfacial regions of the transmembrane domain (TMD) reduce POPG binding, and a subset of these mutations increase ELIC desensitization. In contrast, a mutation that decreases ELIC desensitization, increases POPG binding. The results support a mechanism by which POPG stabilizes the open state of ELIC relative to the desensitized state by direct binding at specific sites
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Evolution of oceanic near surface stratification in response to an autumn storm
Understanding the processes that control the evolution of the ocean surface boundary layer (OSBL) is a prerequisite for obtaining accurate simulations of air-sea fluxes of heat and trace gases. Observations of the rate of dissipation of turbulent kinetic energy (É›), temperature, salinity, current structure and wave-field over a period of 9.5 days in the NE Atlantic during the Ocean Surface Mixing, Ocean Submesoscale Interaction Study (OSMOSIS), are presented. The focus of this study is a storm which passed over the observational area during this period. The profiles of É› in the OSBL are consistent with profiles from large eddy simulation (LES) of Langmuir turbulence. In the transition layer (TL), at the base of the OSBL, É› was found to vary periodically at the local inertial frequency. A simple bulk model of the OSBL and a parametrisation of shear driven turbulence in the TL are developed. The parametrisation of É› is based on assumptions about the momentum balance of the OSBL and shear across the TL. The predicted rate of deepening, heat budget and the inertial currents in the OSBL were in good agreement with the observations, as is the agreement between the observed value of É› and that predicted using the parametrisation. A previous study reported spikes of elevated dissipation related to enhanced wind-shear alignment at the base of the OSBL after this storm. The spikes in dissipation are not predicted by this new parametrisation, implying that they are not an important source of dissipation during the storm
An annual cycle of submesoscale vertical flow and restratification in the upper ocean
Numerical simulations suggest that submesoscale turbulence may transform lateral buoyancy gradients into vertical stratification, and thus restratify the upper ocean via vertical flow. However, the observational evidence for this restratifying process has been lacking due to the difficulty in measuring such ephemeral phenomena, particularly over periods of months to years. This study presents an annual cycle of the vertical velocity and associated restratification estimated from two nested clusters of meso- and submesoscale-resolving moorings, deployed in a typical mid-ocean area of the Northeast Atlantic. Vertical velocities inferred using the non-diffusive density equation are substantially stronger at submesoscales (horizontal scales of 1-10 km) than at mesoscales (horizontal scales of 10-100 km), with respective root mean square values of 38.0 ± 6.9 m/day and 22.5 ± 3.3 m/day. The largest submesoscale vertical velocities and rates of restratification occur in events of a few days’ duration in winter and spring, and extend down to at least 200 m below the mixed layer base. These events commonly coincide with the enhancement of submesoscale lateral buoyancy gradients, which is itself associated with persistent mesoscale frontogenesis. This suggests that mesoscale frontogenesis is a regular precursor of the submesoscale turbulence that restratifies the upper ocean. The upper-ocean restratification induced by submesoscale motions integrated over the annual cycle is comparable in magnitude to the net destratification driven by local atmospheric cooling, indicating that submesoscale flows play a significant role in determining the climatological upper-ocean stratification in the study area
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Seasonality of submesoscale flows in the ocean surface boundary layer
A signature of submesoscale flows in the upper ocean is skewness in the distribution of relative vorticity. Expected to result for high Rossby-number flows, such skewness has implications for mixing, dissipation and stratification within the upper ocean. An array of moorings deployed in the Northeast Atlantic for one year as part of the OSMOSIS experiment reveals that relative vorticity is positively skewed during winter even though the scale of the Rossby number is less than 0.5. Furthermore, this skewness is reduced to zero during spring and autumn. There is also evidence of modest seasonal variations in the gradient Rossby number. The proposed mechanism by which relative vorticity is skewed is that the ratio of lateral to vertical buoyancy gradients, as summarized by the inverse gradient Richardson number, restricts its range during winter but less so at other times of the year. These results support recent observations and model simulations suggesting the upper ocean is host to a seasonal cycle in submesoscale turbulence
Submesoscale Instabilities in Mesoscale Eddies
Submesoscale processes have been extensively studied in observations and simulations of fronts. Recent idealized simulations show that submesoscale instabilities also occur in baroclinic mesoscale cyclones and anticyclones. The instabilities in the anticyclone grow faster and at coarser grid resolution than in the cyclone. The instabilities lead to larger restratification in the anticyclone than in the cyclone. The instabilities also lead to changes in the mean azimuthal jet around the anticyclone from 2-km resolution, but a similar effect only occurs in the cyclone at 0.25-km resolution. A numerical passive tracer experiment shows that submesoscale instabilities lead to deeper subduction in the interior of anticyclonic than cyclonic eddies because of outcropping isopycnals extending deeper into the thermocline in anticyclones. An energetic analysis suggests that both vertical shear production and vertical buoyancy fluxes are important in anticyclones but primarily vertical buoyancy fluxes occur in cyclones at these resolutions. The energy sources and sinks vary azimuthally around the eddies caused by the asymmetric effects of the Ekman buoyancy flux. Glider transects of a mesoscale anticyclone in the Tasman Sea show that water with low stratification and high oxygen concentrations is found in an anticyclone, in a manner that may be consistent with the model predictions for submesoscale subduction in mesoscale eddies
Living donor liver transplant from an HIV-positive mother to her HIV-negative child : opening up new therapeutic options
OBJECTIVE : Transplant a liver from an HIV-positive mother to her HIV-negative child to
save the child’s life.
DESIGN : A unique case of living donor liver transplantation from an HIV-positive
mother to her HIV-negative child in South Africa. Two aspects of this case are
ground-breaking. First, it involves living donation by someone who is HIVpositive
and second it involves controlled transplant of an organ from an HIV-positive
donor into an HIV-negative recipient, with the potential to prevent infection in the
recipient.
METHODS : Standard surgical procedure for living donor liver transplantation at our
centre was followed. HIV-prophylaxis was administered preoperatively. Extensive,
ultrasensitive HIV testing, over and above standard diagnostic assays, was undertaken
to investigate recipient serostatus and is ongoing.
RESULTS : Both mother and child are well, over 1 year posttransplantation. HIV seroconversion
in our recipient was detected with serological testing at day 43 posttransplant.
However, a decline in HIV antibody titres approaching undetectable levels is now being
observed. No plasma, or cell-associated HIV-1 DNA has been detected in the recipient
at any time-point since transplant.
CONCLUSION : This case potentially opens up a new living liver donor pool which might
have clinical relevance in countries where there is a high burden of HIV and a limited
number of deceased donor organs or limited access to transplantation. However, our
recipient’s HIV status is equivocal at present and additional investigation regarding
seroconversion events in this unique profile is ongoing.The South African
Research Chairs Initiative of the Department of Science
and Technology and National Research Foundation of
South Africa.http://journals.lww.com/aidsonlineam2019Medical Virolog