Absolute Frequency Atlas from 915 nm to 985 nm based on Laser Absorption Spectroscopy of Iodine

This article reports on laser absorption spectroscopy of iodine gas between 915 nm and 985 nm. This wavelength range is scanned utilizing a narrow linewidth and mode-hop-free tunable diode-laser whose frequency is actively controlled using a calibrated wavelength meter. This allows us to provide an iodine atlas that contains almost 10,000 experimentally observed reference lines with an uncertainty of 50 MHz. For common lines, good agreement is found with a publication by Gerstenkorn et al. [S. Gerstenkorn, P. Luc, Atlas du spectre d'absorption de la molecule d'iode 14800-20000 cm$^{-1}$, Paris: Editions du Centre National de la Recherche Scientifique (CNRS), 1978]. The new rich dataset allows existing models of the iodine molecule to be refined and can serve as a reference for laser frequency calibration and stabilization.Comment: 7 pages, 4 figures, link to online data depositor

Additional file 3: of Copy number variations in Friesian horses and genetic risk factors for insect bite hypersensitivity

CNVs randomly selected based on incidence and size validated through qPCR. CNVR identification, chromosome (ECA), start and end position (in bp) and size of the CNVs in the investigated Friesians horse sample is presented, including information on whether the CNV concerned a private (present in 1 horse) or shared (present in 2 horses; the exact same breakpoints were observed) CNV. The designed primers, state of the CNV and results of the qPCR are given. (DOCX 14 kb

Additional file 6: of Copy number variations in Friesian horses and genetic risk factors for insect bite hypersensitivity

Chromosomal distribution, characteristics and enrichment of detected CNVs and CNVRs. Number of CNVs and CNVRs detected per Equus caballus chromosome (ECA), including detection, state, content, mean size (in base pairs), coverage (in base pairs), chromosomal distribution ( = number of CNVs per chromosome total number of CNVs × 100 % $$=\frac{number\kern0.5em of\kern0.5em CNVs\kern0.5em per\kern0.5em chromosome}{total\kern0.5em number\kern0.5em of\kern0.5em CNVs}\times \kern0.5em 100\%$$ ), chromosomal coverage ( CNVR coverage per chromosome length of chromosome × 100 % $$\frac{CNVR\kern0.5em coverage\kern0.5em per\kern0.5em chromosome}{length\kern0.5em of\kern0.5em chromosome}\times 100\%$$ ) and SNP coverage in base pairs ( = length of chromosome number of SNPs per chromosome $$=\frac{length\kern0.5em of\kern0.5em chromosome}{number\kern0.5em of\kern0.5em SNPs\kern0.5em per\kern0.5em chromosome}$$ ). (DOCX 24 kb

Additional file 2: of Copy number variations in Friesian horses and genetic risk factors for insect bite hypersensitivity

Multidimensional scaling plot of 276 genotyped Friesian horses. Multidimensional scaling plot of 276 genotyped Friesian horses calculated with cluster and mds-plot commands in PLINK software v1.07 [28, 29] using autosomal SNPs. (DOCX 31 kb

Additional file 4: of Copy number variations in Friesian horses and genetic risk factors for insect bite hypersensitivity

Regional association plot (ECA20) of insect bite hypersensitivity in Friesian horses. Significance level based on allele frequency differences between cases (n = 141) and controls (n = 135) using a χ2-test (1df). The horizontal red line is the Bonferroni corrected significance level (P-value = 1.63 × 10− 7). (DOCX 27 kb

Additional file 7: of Copy number variations in Friesian horses and genetic risk factors for insect bite hypersensitivity

5350 CNVRs detected by CNVRuler based on 15,041 CNVs identified by PennCNV in 222 Friesian horses. 5350 CNVRs detected by CNVRuler based on 15,041 CNVs identified in PennCNV [33–35] in 222 Friesian horses. Information that is presented includes CNVR identification (CNVR_ID), Equus caballus chromosome (ECA), start position (in bp), end position (in bp), size (in bp), copy number state, number of horses (N) with CNVR and whether the CNVR includes a gene(s) and is present in 1 horse (private) or more (shared). The gene(s) located within the CNVR were identified using human orthologues. Start and end position of the genes annotated in the horse genome are presented, including Ensembl IDs and whether the CNVR is upstream, inside, downstream of the gene or encompasses the gene. (XLSX 603 kb

Additional file 9: of Copy number variations in Friesian horses and genetic risk factors for insect bite hypersensitivity

Visualization of individual CNVs within the CNVRs with the lowest P-value in the association tests. Visualization of individual CNVs within the CNVR on ECA10:12,948,489-13,075,518 (association test including both gains and losses), ECA20:30,624,048-30,689,273 (association test including gains only) and ECA20:30,743,179-30,775,429 (association test including losses only). Each row represents one horse and the X-axis is the position on the chromosome. The black line marks the location of the CNVR. Blue lines represent controls, red lines cases. A dotted line represents a deletion (state = 0n), a striped line represents a CNV with state equals 1n and a solid line represents a duplication (3n). (DOCX 50 kb