The Role of Yeast and Lactic Acid Bacteria in the Production of Fermented Beverages in South America

Fermentation is one of the oldest forms of food preservation in the world. In South America, most fermented beverages are nondairy products featuring several other food raw materials such as cereals, fruits, and vegetables. Generally, natural fermentations are carried out by yeast and lactic acid bacteria forming a complex microbiota that acts in cooperation. Yeast have a prominent role in the production of beverages, due to the ability to accumulate high levels of ethanol and to produce highly desirable aroma compounds, but lactic acid bacteria are particularly important in fermentation because they produce desirable acids, flavor compounds, and peptides that inhibit the growth of undesirable organisms. Among the South America beverages based on cereals and vegetables, the fermented beverages chicha, caxiri, cauim and champús, and cachaça, a fermented and distilled beverage, could be cited. Genetic and physiological analyses of Saccharomyces cerevisiae strains isolated from cachaça have been shown to present interesting traits for beer production, such as flocculation and production of aroma compounds, fundamental to high-quality beer. The study of these traditional beverages allows the identification of new microorganism strains displaying enhanced resistance or new flavor and aroma profiles that could lead to applications in several industries and ultimately new products

Host Susceptibility to Brucella abortus Infection Is More Pronounced in IFN-γ knockout than IL-12/β2-Microglobulin Double-Deficient Mice

Brucella abortus is a facultative intracellular bacterial pathogen that causes abortion in domestic animals and undulant fever in humans. IFN-γ, IL-12, and CD8+ T lymphocytes are important components of host immune responses against B. abortus. Herein, IFN-γ and IL-12/β2-microglobulin (β2-m) knockout mice were used to determine whether CD8+ T cells and IL-12-dependent IFN-γ deficiency would be more critical to control B. abortus infection compared to the lack of endogenous IFN-γ. At 1 week after infection, IFN-γ KO and IL-12/β2-m KO mice showed increased numbers of bacterial load in spleens; however, at 3 weeks postinfection (p.i.), only IFN-γ KO succumbed to Brucella. All IFN-γ KO had died at 16 days p.i. whereas death within the IL-12/β2-m KO group was delayed and occurred at 32 days until 47 days postinfection. Susceptibility of IL-12/β2-m KO animals to Brucella was associated to undetectable levels of IFN-γ in mouse splenocytes and inability of these cells to lyse Brucella-infected macrophages. However, the lack of endogenous IFN-γ was found to be more important to control brucellosis than CD8+ T cells and IL-12-dependent IFN-γ deficiencies

Behavior of the training load, recovery and well-being in volleyball professional athletes in weeks with and without matches

O objetivo deste estudo foi comparar a carga interna de treinamento, o estado de recuperação e o bem-estar em atletas profissionais de Voleibol de acordo com a frequência semanal de jogos. A recuperação foi quantificada pela Escala de TQR, a carga pela PSE da sessão e o bem-estar pela escala de QBE. Para comparar os valores da Carga de Treinamento Semanal Total, o delta da Qualidade Total de Recuperação (ΔTQR) e o delta da Qualidade de bem-estar (ΔQBE) foi aplicado o teste ANOVA para medidas repetidas. Não foram encontradas diferenças significativas ao comparar a carga de treinamento com a frequência de jogos semanais, porém o tamanho do efeito foi muito elevado quando se comparou a diferença entre um e dois jogos semanais, assim como de nenhum a dois jogos na semana. Houve diferença entre o ΔTQR entre as semanas sem jogos e com dois jogos assim como entre as semanas com um e dois jogos. Já o ΔQBE foi diferente para as semanas que não havia a realização de jogos e aquelas com jogos. Concluise que, mesmo que não haja uma variação das cargas com diferentes frequências semanais de jogos, a recuperação assim como o bem-estar dos atletas foi alterada.The aim of this study was to compare the internal training load, recovery state and the well-being of volleyball professional athletes, according to the number of matches during the week. The recovery was quantified with TQR Scale, the training load with sRPE and the well-being with QBE Scale. The ANOVA was applied for repeated measures in order to compare the values of Total weekly training load, the delta of Total Quality Recovery (ΔTQR) and the delta of the quality of well-being (ΔQBE),. There was no significant difference among the training load and the frequency of weekly games, but the effect size was very high when it was compared to the difference between one and two weekly games, as well as any and two games in a week. There were differences on the ΔTQR between weeks without games and with two games, as well as weeks with one and two matches. The ΔQBE was different for weeks in which there was no game and those with matches, regardless of the frequency. It was concluded that, even if there is no variation of loads in different weekly frequency of matches, the athletes' recovery and well-being change.Facultad de Humanidades y Ciencias de la Educació

Behavior of the training load, recovery and well-being in volleyball professional athletes in weeks with and without matches

O objetivo deste estudo foi comparar a carga interna de treinamento, o estado de recuperação e o bem-estar em atletas profissionais de Voleibol de acordo com a frequência semanal de jogos. A recuperação foi quantificada pela Escala de TQR, a carga pela PSE da sessão e o bem-estar pela escala de QBE. Para comparar os valores da Carga de Treinamento Semanal Total, o delta da Qualidade Total de Recuperação (ΔTQR) e o delta da Qualidade de bem-estar (ΔQBE) foi aplicado o teste ANOVA para medidas repetidas. Não foram encontradas diferenças significativas ao comparar a carga de treinamento com a frequência de jogos semanais, porém o tamanho do efeito foi muito elevado quando se comparou a diferença entre um e dois jogos semanais, assim como de nenhum a dois jogos na semana. Houve diferença entre o ΔTQR entre as semanas sem jogos e com dois jogos assim como entre as semanas com um e dois jogos. Já o ΔQBE foi diferente para as semanas que não havia a realização de jogos e aquelas com jogos. Concluise que, mesmo que não haja uma variação das cargas com diferentes frequências semanais de jogos, a recuperação assim como o bem-estar dos atletas foi alterada.The aim of this study was to compare the internal training load, recovery state and the well-being of volleyball professional athletes, according to the number of matches during the week. The recovery was quantified with TQR Scale, the training load with sRPE and the well-being with QBE Scale. The ANOVA was applied for repeated measures in order to compare the values of Total weekly training load, the delta of Total Quality Recovery (ΔTQR) and the delta of the quality of well-being (ΔQBE),. There was no significant difference among the training load and the frequency of weekly games, but the effect size was very high when it was compared to the difference between one and two weekly games, as well as any and two games in a week. There were differences on the ΔTQR between weeks without games and with two games, as well as weeks with one and two matches. The ΔQBE was different for weeks in which there was no game and those with matches, regardless of the frequency. It was concluded that, even if there is no variation of loads in different weekly frequency of matches, the athletes' recovery and well-being change.Facultad de Humanidades y Ciencias de la Educació

Behavior of the training load, recovery and well-being in volleyball professional athletes in weeks with and without matches

O objetivo deste estudo foi comparar a carga interna de treinamento, o estado de recuperação e o bem-estar em atletas profissionais de Voleibol de acordo com a frequência semanal de jogos. A recuperação foi quantificada pela Escala de TQR, a carga pela PSE da sessão e o bem-estar pela escala de QBE. Para comparar os valores da Carga de Treinamento Semanal Total, o delta da Qualidade Total de Recuperação (ΔTQR) e o delta da Qualidade de bem-estar (ΔQBE) foi aplicado o teste ANOVA para medidas repetidas. Não foram encontradas diferenças significativas ao comparar a carga de treinamento com a frequência de jogos semanais, porém o tamanho do efeito foi muito elevado quando se comparou a diferença entre um e dois jogos semanais, assim como de nenhum a dois jogos na semana. Houve diferença entre o ΔTQR entre as semanas sem jogos e com dois jogos assim como entre as semanas com um e dois jogos. Já o ΔQBE foi diferente para as semanas que não havia a realização de jogos e aquelas com jogos. Concluise que, mesmo que não haja uma variação das cargas com diferentes frequências semanais de jogos, a recuperação assim como o bem-estar dos atletas foi alterada.The aim of this study was to compare the internal training load, recovery state and the well-being of volleyball professional athletes, according to the number of matches during the week. The recovery was quantified with TQR Scale, the training load with sRPE and the well-being with QBE Scale. The ANOVA was applied for repeated measures in order to compare the values of Total weekly training load, the delta of Total Quality Recovery (ΔTQR) and the delta of the quality of well-being (ΔQBE),. There was no significant difference among the training load and the frequency of weekly games, but the effect size was very high when it was compared to the difference between one and two weekly games, as well as any and two games in a week. There were differences on the ΔTQR between weeks without games and with two games, as well as weeks with one and two matches. The ΔQBE was different for weeks in which there was no game and those with matches, regardless of the frequency. It was concluded that, even if there is no variation of loads in different weekly frequency of matches, the athletes' recovery and well-being change.Facultad de Humanidades y Ciencias de la Educació

Draft genome sequence of Wickerhamomyces anomalus LBCM1105, isolated from cachaça fermentation

Wickerhamomyces anomalus LBCM1105 is a yeast isolated from cachaça distillery fermentation vats, notable for exceptional glycerol consumption ability. We report its draft genome with 20.5x in-depth coverage and around 90% extension and completeness. It harbors the sequences of proteins involved in glycerol transport and metabolism.The authors gratefully acknowledge Laboratorio Nacional de Ciencia e Tecnologia do Bioetanol (CTBE) and the Centro Nacional de Pesquisa em Energia e Materiais (CNPEM) for support with the sequencing of LBCM1105. This work was supported by CAPES/Brazil (PNPD 2755/2011; PCF-PVE 021/2012), by CNPq (Brazil), processes 304815/2012 (research grant) and 305135/2015-5, and by AUXPE-PVES 1801/2012 (Process 23038.015294/2016-18) from Brazilian Government and by UFOP. C.L. is supported by the strategic program UID/BIA/04050/2013 [POCI-01-0145-FEDER-007569] funded by national funds through the FCT I.P. and by the ERDF through the COMPETE2020 - Programa Operacional de Competitividade e Internacionalizacao (POCI). DMRP is a fellow from the CNPq (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico) - Brazil (310080/2018-5)