Tailoring explicit time-marching schemes to improve convergence characteristics

Multi-stage time-stepping schemes, tailored to chosen spatial-differencing operators, are derived and tested. The schemes are constructed to give optimal damping of the high-frequency waves. They are ideal for use with multi-grid acceleration. The concept of characteristic time-stepping, necessary for the extension of the scalar analysis to systems of equations, is presented. The schemes show a marked improvement over Runge-Kutta schemes

Towards a genuinely multi-dimensional upwind scheme

Methods of incorporating multi-dimensional ideas into algorithms for the solution of Euler equations are presented. Three schemes are developed and tested: a scheme based on a downwind distribution, a scheme based on a rotated Riemann solver and a scheme based on a generalized Riemann solver. The schemes show an improvement over first-order, grid-aligned upwind schemes, but the higher-order performance is less impressive. An outlook for the future of multi-dimensional upwind schemes is given

Chondrogenic potential of human articular chondrocytes and skeletal stem cells: a comparative study

Regenerative medicine strategies have increasingly focused on skeletal stem cells (SSCs), in response to concerns such as donor site morbidity, dedifferentiation and limited lifespan associated with the use of articular chondrocytes for cartilage repair. The suitability of SSCs for cartilage regeneration, however, remains to be fully determined. This study has examined the chondrogenic potential of human STRO-1-immunoselected SSCs (STRO-1+ SSCs), in comparison to human articular chondrocytes (HACs), by utilising two bioengineering strategies, namely ‘‘scaffold-free’’ three-dimensional(3-D) pellet culture and culture using commercially available, highly porous, 3-D scaffolds with interconnected pore networks. STRO-1+ SSCs were isolated by magnetic-activated cell sorting from bone marrow samples of haematologically normal osteoarthritic individuals following routine hip replacement procedures. Chondrocytes were isolated by sequential enzymatic digestion of deep zone articular cartilage pieces dissected from femoral heads of the same individuals. After expansion in monolayer cultures, the harvested cell populations were centrifuged to form high-density 3-D pellets and also seeded in the 3-D scaffold membranes, followed by culture in serum-free chondrogenic media under static conditions for 21 and 28 days, respectively. Chondrogenic differentiation was determined by gene expression,histological and immunohistochemical analyses. Robust cartilage formation and expression of hyaline cartilage-specific markers were observed in both day-21 pellets and day-28 explants generated using HACs. In comparison, STRO-1+ SSCs demonstrated significantly lower chondrogenic differentiation potential and a tendency for hypertrophic differentiation in day-21 pellets. Culture of STRO-1+ SSCs in the 3-D scaffolds improved the expression of hyaline cartilage-specific markers in day-28 explants, however, was unable to prevent hypertrophic differentiation of the SSC population. The advantages of application of SSCs in tissue engineering are widely recognised; the results of this study, however, highlight the need for further development of cell culture protocols that may otherwise limit the application of this stem cell population in cartilage bioengineering strategies

Superstability of Surface Nanobubbles

Shock wave induced cavitation experiments and atomic force microscopy measurements of flat polyamide and hydrophobized silicon surfaces immersed in water are performed. It is shown that surface nanobubbles, present on these surfaces, do not act as nucleation sites for cavitation bubbles, in contrast to the expectation. This implies that surface nanobubbles are not just stable under ambient conditions but also under enormous reduction of the liquid pressure down to −6MPa. We denote this feature as superstability.Comment: 5 pages, 2 figure

Modelling bispecific monoclonal antibody interaction with two cell membrane targets indicates the importance of surface diffusion

We have developed a mathematical framework for describing a bispecific monoclonal antibody interaction with two independent membrane-bound targets that are expressed on the same cell surface. The bispecific antibody in solution binds either of the two targets first, and then cross-links with the second one whilst on the cell surface, subject to rate-limiting lateral diffusion step within the lifetime of the monovalently engaged antibody-antigen complex. At experimental densities, only a small fraction of the free targets is expected to lie within the reach of the antibody binding sites at any time. Using ordinary differential equation and Monte Carlo simulation-based models, we validated this approach against an independently published anti-CD4/CD70 DuetMab experimental data set. As a result of dimensional reduction, the cell surface reaction is expected to be so rapid that, in agreement with the experimental data, no monovalently bound bispecific antibody binary complexes accumulate until cross-linking is complete. The dissociation of the bispecific antibody from the ternary cross-linked complex is expected to be significantly slower than that from either of the monovalently bound variants. We estimate that the effective affinity of the bivalently bound bispecific antibody is enhanced for about four orders of magnitude over that of the monovalently bound species. This avidity enhancement allows for the highly specific binding of anti-CD4/CD70 DuetMab to the cells that are positive for both target antigens over those that express only one or the other We suggest that the lateral diffusion of target antigens in the cell membrane also plays a key role in the avidity effect of natural antibodies and other bivalent ligands in their interactions with their respective cell surface receptors

Intrinsic 1/f device noise reduction and its effect on phase noise in CMOS ring oscillators

This paper gives experimental proof of an intriguing physical effect: periodic on-off switching of MOS transistors in a CMOS ring oscillator reduces their intrinsic 1/f noise and hence the oscillator's close-in phase noise. More specifically, it is shown that the 1/f3 phase noise is dependent on the gate-source voltage of the MOS transistors in the off state. Measurement results, corrected for waveform-dependent upconversion and effective bias, show an 8-dB-lower 1/f3 phase noise than expected. It will be shown that this can be attributed to the intrinsic 1/f noise reduction effect due to periodic on-off switchin

Simulation of unsteady inviscid flow on an adaptively refined Cartesian grid

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/76130/1/AIAA-1992-443-336.pd

Extinction curve template for intrinsically reddened quasars

We analyze the near-infrared to UV data of 16 quasars with redshifts ranging from 0.71 $<$ $z$ $<$ 2.13 to investigate dust extinction properties. The sample presented in this work is obtained from the High $A_V$ Quasar (HAQ) survey. The quasar candidates were selected from the Sloan Digital Sky Survey (SDSS) and the UKIRT Infrared Deep Sky Survey (UKIDSS), and follow-up spectroscopy was carried out at the Nordic Optical Telescope (NOT) and the New Technology Telescope (NTT). To study dust extinction curves intrinsic to the quasars, from the HAQ survey we selected 16 cases where the Small Magellanic Cloud (SMC) law could not provide a good solution to the spectral energy distributions (SEDs). We derived the extinction curves using Fitzpatrick & Massa 1986 (FM) law by comparing the observed SEDs to the combined quasar template from Vanden Berk et al. 2001 and Glikman et al. 2006. The derived extinction, $A_V$, ranges from 0.2-1.0 mag. All the individual extinction curves of our quasars are steeper ($R_V=2.2$-2.7) than that of the SMC, with a weighted mean value of $R_V=2.4$. We derive an `average quasar extinction curve' for our sample by fitting SEDs simultaneously by using the weighted mean values of the FM law parameters and a varying $R_V$. The entire sample is well fit with a single best-fit value of $R_V=2.2\pm0.2$. The `average quasar extinction curve' deviates from the steepest Milky Way and SMC extinction curves at a confidence level $\gtrsim95\%$. Such steep extinction curves suggest a significant population of silicates to produce small dust grains. Moreover, another possibility could be that the large dust grains may have been destroyed by the activity of the nearby active galactic nuclei (AGN), resulting in steep extinction curves.Comment: 8 pages, 4 figures, 1 tabl