Serendipitous Data Following a Severe Windstorm in an Old-Growth Pine Stand

Reliable dimensional data for old-growth pine-dominated forests in the Gulf Coastal Plain of Arkansas are hard to find, but sometimes unfortunate circumstances provide good opportunities to acquire this information. On July 11, 2013, a severe thunderstorm with high winds struck the Levi Wilcoxon Demonstration Forest (LWDF) near Hamburg, Arkansas. This storm uprooted or snapped dozens of large pines and hardwoods and provided an opportunity to more closely inspect these rare specimens. For instance, the largest tree killed in this event, a loblolly pine (Pinus taeda), was 105 cm in diameter at breast height, 39.3 m tall, and if the tree had been sound would have yielded 3,803 board feet (Doyle log rule) of lumber. Gross board foot volume yield was also estimated from two other recently toppled large pines, an 85-cm-DBH loblolly and an 86-cm-DBH shortleaf pine (Pinus echinata), which tallied 2,430 and 2,312 board feet Doyle, respectively. A number of the other wind thrown pines on the LWDF were sound enough to count their rings for a reasonable (± 2-5 years) estimate of their ages. The stump of the fallen national champion shortleaf pine had 168 rings, and counts from other pines toppled by this storm had from 68 to 198 rings. We also searched for a new champion shortleaf pine using a LiDAR canopy height model of the LWDF to narrow our search. This preliminary assessment produced a number of targets that exceeded 40 m in height; further field checking of the tallest of these trees found that these were loblolly pines up to about 44 m. We eventually found shortleaf pines between 37 and 41 m tall, with diameters of up to 85 cm, indicating that the LWDF could still contain the Arkansas state champion

Multiscale fluid--particle thermal interaction in isotropic turbulence

We use direct numerical simulations to investigate the interaction between the temperature field of a fluid and the temperature of small particles suspended in the flow, employing both one and two-way thermal coupling, in a statistically stationary, isotropic turbulent flow. Using statistical analysis, we investigate this variegated interaction at the different scales of the flow. We find that the variance of the fluid temperature gradients decreases as the thermal response time of the suspended particles is increased. The probability density function (PDF) of the fluid temperature gradients scales with its variance, while the PDF of the rate of change of the particle temperature, whose variance is associated with the thermal dissipation due to the particles, does not scale in such a self-similar way. The modification of the fluid temperature field due to the particles is examined by computing the particle concentration and particle heat fluxes conditioned on the magnitude of the local fluid temperature gradient. These statistics highlight that the particles cluster on the fluid temperature fronts, and the important role played by the alignments of the particle velocity and the local fluid temperature gradient. The temperature structure functions, which characterize the temperature fluctuations across the scales of the flow, clearly show that the fluctuations of the fluid temperature increments are monotonically suppressed in the two-way coupled regime as the particle thermal response time is increased. Thermal caustics dominate the particle temperature increments at small scales, that is, particles that come into contact are likely to have very large differences in their temperature. This is caused by the nonlocal thermal dynamics of the particles..

Bacterial Quality of Private Water Wells in Clark County, Arkansas

Most private water wells in Clark County appeared to be contaminated by bacteria, apparently entering the wells from surface water seepage. Eighteen to 24% of the wells investigated were positive for fecal contamination. Deeper wells were less often contaminated. More than one-half of the wells sampled exceeded recommended limits of inorganic chemicals for safe potable water. High concentrations of iron and manganese were most common, exceeding recommended limits in more than 40% of the well

Irreversibility-inversions in 2 dimensional turbulence

In this paper we consider a recent theoretical prediction (Bragg \emph{et al.}, Phys. Fluids \textbf{28}, 013305 (2016)) that for inertial particles in 2D turbulence, the nature of the irreversibility of the particle-pair dispersion inverts when the particle inertia exceeds a certain value. In particular, when the particle Stokes number, ${\rm St}$, is below a certain value, the forward-in-time (FIT) dispersion should be faster than the backward-in-time (BIT) dispersion, but for ${\rm St}$ above this value, this should invert so that BIT becomes faster than FIT dispersion. This non-trivial behavior arises because of the competition between two physically distinct irreversibility mechanisms that operate in different regimes of ${\rm St}$. In 3D turbulence, both mechanisms act to produce faster BIT than FIT dispersion, but in 2D turbulence, the two mechanisms have opposite effects because of the flux of energy from the small to the large scales. We supplement the qualitative argument given by Bragg \emph{et al.} (Phys. Fluids \textbf{28}, 013305 (2016)) by deriving quantitative predictions of this effect in the short time limit. We confirm the theoretical predictions using results of inertial particle dispersion in a direct numerical simulation of 2D turbulence. A more general finding of this analysis is that in turbulent flows with an inverse energy flux, inertial particles may yet exhibit a net downscale flux of kinetic energy because of their non-local in-time dynamics

Site-directed mutagenesis of the proton-pumping pyridine nucleotide transhydrogenase of Escherichia coli

AbstractThe pyridine nucleotide transhydrogenase of Escherichia coli catalyzes the reversible transfer of hydride ion equivalents between NAD+ and NADP+ coupled to the translocation of protons across the cytoplasmic membrane. It is composed of two subunits (α, β) organized as an α2β2 tetramer. This brief review describes the use of site-directed mutagenesis to investigate the structure, mechanism and assembly of the transhydrogenase. This technique has located the binding sites for NAD(H) and NADP(H) in the α and β subunits, respectively. Mutagenesis has shown that the cysteine residues of the enzyme are not essential for its function, and that inhibition of the enzyme by sulfhydryl-specific reagents must be due to perturbation of the three-dimensional structure. The sites of reaction of the inhibitors N,N′-dicyclohexylcarbodiimide and N-(1-pyrene)maleimide have been located. Selective mutation and insertion of cysteine residues followed by cupric o-phenanthrolinate-induced disulfide crosslinking has defined a region of interaction between the α subunits in the holoenzyme. Determination of the accessibility of selectively inserted cysteine residues has been used to determine the folding pattern of the transmembrane helices of the β subunit. Site-directed matagenesis of the transmembrane domain of the β subunit has permitted the identification of histidine, aspartic acid and asparagine residues which are part of the proton-pumping pathway of the transhydrogenase. Site-directed matagenesis and amino acid deletions have shown that the six carboxy terminal residues of the α subunit and the two carboxy terminal residues of the β subunit are necessary for correct assembly of the transhydrogenase in the cytoplasmic membrane

Modeling Loblolly Pine Aboveground Live Biomass in a Mature Pine-hardwood Stand: A Cautionary Tale

Carbon sequestration in forests is a growing area of interest for researchers and land managers. Calculating the quantity of carbon stored in forest biomass seems to be a straightforward task, but it is highly dependent on the function(s) used to construct the stand. For instance, there are a number of possible equations to predict aboveground live biomass for loblolly pine (Pinus taeda) growing in southeastern Arkansas. Depending on stem diameter at breast height (DBH), biomass varied considerably between four different prediction systems for loblolly pine. According to the tested models, individual tree oven-dry biomass for a 50 cm DBH loblolly pine ranged between 1,085 kg and 1,491 kg. Beyond this point, departures between these models became increasingly pronounced, with one even projecting an irrational decline to negative biomass for trees \u3e 138.7 cm DBH, while the others varied between 12,447 and 15,204 kg. Although some deviation is not surprising given the inherent differences in model form and three of the models were extrapolations across much of this diameter range, the difference between the extremes was unexpected. Such disparities significantly impact stand-level (cumulative) predictions of biomass in forests dominated by large-diameter individuals, as demonstrated for an existing stand (Hyatt’s Woods) in Drew County, Arkansas. Differences between these models caused loblolly pine aboveground live-tree biomass estimations in Hyatt’s Woods to vary by almost 34,000 kg/ha