Heterologous screening of hybridomas for the development of broad-specific monoclonal antibodies against deoxynivalenol and its analogues

Hapten heterology was introduced into the steps of hybridoma selection for the development of monoclonal antibodies (MAbs) against deoxynivalenol (DON). Firstly, a novel heterologous DON hapten was synthesised and covalently coupled to proteins (i.e. bovine serum albumin (BSA), ovalbumin and horseradish peroxidase) using the linkage of cyanuric chloride (CC). After immunisation, antisera from different DON immunogens were checked for the presence of useful antibodies. Next, both homologous and heterologous enzyme-linked immunosorbent assays were conducted to screen for hybridomas. It was found that heterologous screening could significantly reduce the proportion of false positives and appeared to be an efficient approach for selecting hybridomas of interest. This strategy resulted in two kinds of broad-selective MAbs against DON and its analogues. They were quite distinct from other reported DON-antibodies in their cross-reactivity profiles. A unique MAb 13H1 derived from DON-CC-BSA immunogen could recognise DON and its analogues in the order of HT-2 toxin > 15-acetyl-DON > DON > nivalenol, with IC50 ranging from 1.14 to 7.69 mu g/ml. Another preferable MAb 10H10 generated from DON-BSA immunogen manifested relatively similar affinity to DON, 3-acetyl-DON and 15-acetyl-DON, with IC50 values of 22, 15 and 34 ng/ml, respectively. This is the first broad-specific MAb against DON and its two acetylated forms and thus it can be used for simultaneous detection of the three mycotoxins

Refusing Moral Panics/Defending Education and the Right to Protest

Invoking “indications” of “pro-Palestinian violence”, Dutch authorities partially succeeded in demobilizing a national protest against the severe higher education budget cuts. According to Sarah Bracke, this is not an isolated incident. Throughout history and around the globe, far-right political positions are shaped by anti-intellectualism and invested in curtailing educational institutions. And moral panics — in this case “pro-Palestinian violence” — are always used in the process.<br/

Agriculture/ Graduate Students/ Carlson & Bracke/ Purdue University/ 2014

Librarians at Purdue University had the opportunity to develop a semester long data information literacy program to ten students in the College of Agriculture. The program was run as a pilot to test out ideas and approaches in teaching data literacy competencies to students. Topics included high level subjects such as data lifecycles, data management planning, metadata and sharing data, along with practical issues such as file naming conventions, developing a metadata record for a data set and depositing data into a repository. Materials in this case study include weekly lesson plans, slide decks, assignments, an application and other documents generated to support the pilot program