Comprehensive overview of the analytical methods for determining pyrrolizidine alkaloids and their derived oxides in foods

Producción CientíficaPyrrolizidine alkaloids and their derived oxides are toxins naturally produced by plants when they are exposed to stress factors. In particular, the unsaturated pyrrolizidine alkaloids exhibiting a double bond between the 1,2-positions have caused great interest in recent years since they have pneumotoxic, genotoxic and carcinogenic effects upon ingestion. In this review, focus on their chemical structure justifying their toxicity is provided as well as insight on the recently adopted EU regulatory framework. In addition, to reveal current trends and highlight the research effort in the field, an all-time bibliometric analysis was performed for the first time. It was found that tea, infusions, honey, spices, and cereals are the most common foodstuffs in which pyrrolizidine alkaloids have been detected. In terms of pre-analytical steps, the application of efficient sample preparation is necessary to detect pyrrolizidine alkaloids in complex food matrices. Solvent extraction followed by a clean-up stage, e.g., solid-phase extraction was the most applied option. Focusing on the analytical methods, liquid chromatography combined with various mass spectrometry detectors has been the golden standard in the field. Gas chromatography methods were also applied, but the need for analyte derivatisation has hindered their application. All in all, this review provides an overview on the analysis of pyrrolizidine alkaloids and their derived oxides, comprehensively discussing the up-to-date advances and highlighting the analytical challenges to be faced.METROFOOD-CZ research infrastructure project (MEYS Grant No: LM2018100

Vliv probiotik ve výživě telat na hmotnostní přírůstky živé hmotnosti a zdravotní stav

This paper aims to monitor the impact of Lactobacillus sporogenes (LS), Saccharomyces cerevisiae (SC), the combination thereof Lactobacillus sporogenes and Saccharomyces cerevisiae (CLS) on the health status and the live weight gain in calves compared to a control group (C). The experiment took place in the period from March 2022 to March 2023. 100 Holstein heifers in the age from 1 to 56 days were included in the experiment. The differences in live weight gain were significant when the live weight gains were compared in the first 14 days after birth between the CLS vs C group (63,36.72 ± 4.81 vs 59.55 ± 4.55, P 0.05. The impact on decrease and duration of diarrhea was not proved statistically P = 0.0634. However, a tendency to decrease the occurrence and duration thereof was proved. The impact of feed additives on the transmission of passive immunity in calves in their first week of life was not proved as statistically significant.Cílem této studie bylo sledovat vliv Lactobacillus sporogenes (LS), Saccharomyces cerevisiae (SC) a jejich kombinaci Lactobacillus sporogenes and Saccharomyces cerevisiae (CLS) na zdravotní stav a přírůstek živé hmotnosti telat oproti skupině kontrolní (C). Pokus se uskutečnil v období březen 2022 až březen 2023. Do pokusu bylo zařazeno celkem 100 holštýnských jaloviček ve stáří 1 až 56 dní. Rozdíly v přírůstku živé hmotnosti byly významné, pokud byly porovnány hmotnostní přírůstky ve 14. dech po narození mezi skupinou CLS vs C (63,36.72 ± 4.81 vs 59.55 ± 4.55, P < 0.05) a v 56 dnech po narození mezi skupinu CLS vs C, LS vs C a SC vs C (87.34 ± 4.95 kg vs 83.15 ± 5.32 kg, P < 0.01; 86.41 ± 5.34 kg vs 83.15 ± 5.32 kg, P < 0.05 a 85.92 ± 5.86 kg vs 83.15 ± 5.32 kg, P < 0.05). Rozdíly v přírůstku živé hmotnosti mezi pokusnými skupinami nebyly statisticky prokázány P > 0.05. Vliv na snížení výskytu a trvání průjmových onemocnění nebyl statisticky prokázán P = 0.0634, ovšem byla zde prokázána tendence ke snížení jejich výskytů a době trvání. Statisticky významný nebyl prokázán vliv krmných aditiv na přenos pasivní imunity u telat v prvním týdnu života