Superovulatory Ovarian Response in Mangalica Gilts is Not Influenced by Feeding Level

The aim of the study was to compare how different feeding levels affect the ovarian potential of follicular development and oocyte maturation in response to superovulatory treatment in native Mangalica (M, n = 17) compared with Landrace (L, n = 20) pigs. Gilts of both breeds were fed high-energy (HI-2.5 kg) or low-energy (LO - 1.25 kg) feed during oestrus synchronization (15 days of Regumate (R) feeding) till the time of oocyte aspiration (Day 6 after Regumate (R)). Follicular growth was stimulated by the administration of 1000 IU equine choriou gonadotropiu (eCG) 24 h after Regumate (R) treatment, and ovulation was induced by injection of 750 IU human choriou gonadotropiu (hCG) 80 h after eCG adminstration. Ultrasound (US) investigation was done three times (4-10 h before, and 40-44 and 72-74 h after eCG administration) for the observation of follicular development. Oocyte and follicular fluid (FF) were collected endoscopically 34 h after hCG injection. Cumulus-oocyte complexes were evaluated, their morphology determined, and thereafter fixed and stained for chromatin evaluation. Oocytes were classified as meiosis-resumed (germinal vesicle breakdown, diakinesis, metaphase I to anaphase 1) or matured (telophase I and metaphase 11). FF concentrations of oestradiol and progesterone were measured by validated radioimmunoassays. In L gilts, differences were observed between HI and LO in the number of preovulatory follicles (32.3 +/- 10.5 vs 17.1 +/- 12.3, p 0.05). Initial follicular growth was not affected by feeding levels; however, preovulatory follicle size was larger in M (7.1 +/- 0.9 and 6.9 +/- 1.1 turn vs 5.7 +/- 0.7 and 5.5 +/- 0.8 mm; p <0.05). No differences were obtained with relation to mature chromatin configuration in both breeds (L gilts: HI - 70% and LO-67% vs M gilts: HI- 67% and LO - 63%). A twofold higher oestradiol concentration was detected in FF of HI-M and LO-M (29.6 +/- 6.8 and 30.9 +/- 10.3 ng/ml respectively) compared with that of L (16.9 +/- 9.7 and 17.9 +/- 3.6 ng/ml, respectively; p <0.05). The mean FF progesterone level was nearly fivefold higher in M (2020.4 +/- 1056 and 1512.2 +/- 1121.8 ng/ml) compared with L (386.2 +/- 113.7 and 298.8 +/- 125.9 ng/ml, p <0.05). The results indicate an influence of the feeding of altered energy on the number of recruitable preovulatory follicles in modern Landrace but not in native Mangalica breed. Moreover, the follicular steroid hormone milieu differs between Landrace and Mangalica gilts but not depending on feeding levels. Oocyte maturation was not affected by diet

Sincronização da ovulação em fêmeas suínas submetidas ao desmame precoce Synchronization of ovulation in sows in early weaning system

Verificou-se a eficiência de protocolos para sincronizar a ovulação em porcas desmamadas precocemente. Trinta porcas com média de 4,4± 2,0 partos e estádio de lactação de 14,8± 0,7 dias foram distribuídas em três grupos de 10 animais: 1- nenhum tratamento hormonal; 2- 1000 UI de PMSG, via intramuscular (IM), 48h pós-desmame e 0,25mg de GnRH, IM, 72h após a aplicação do PMSG; 3- 1000 UI de PMSG, IM, 48h pós-desmame e 500 UI de hCG, IM, 72h após o PMSG. O momento da ovulação foi detectado por ultra-sonografia transretal. A taxa de sincronização (ovulação até 48h após aplicação de hCG ou GnRH) dos grupos 2 e 3 (94,7%) foi maior (P<0,01) que no grupo controle (40%). Com o uso dos protocolos de sincronização de ovulação, as fêmeas tratadas apresentaram, em relação ao grupo controle, tendência de maior taxa de prenhez (95% vs. 70%; P<0,10) e similares intervalo do desmame ao estro (96,5± 3,0 vs. 130,2± 31,4h) e número de leitões nascidos vivos por fêmea gestante no primeiro cio pós-desmame (10,9± 0,8 vs. 12,0± 0,9). Dessa maneira, os protocolos de sincronização usados neste estudo foram eficientes em sincronizar a ovulação, e podem viabilizar o uso da inseminação artificial em horários predeterminados.<br>The objective of this study was to test the efficiency of two protocols in synchronize the ovulation of sows in early weaning system. Thirty multiparous crossbred (Large-White x Landrace) sows with a mean± SD of 4.4± 2.0 parturitions and 14.8± 0.7 days in lactation were divided into three groups of 10 animals each: group 1- control; group 2- received 1000 IU of PMSG (FOLLIGON® , INTERVET) IM, 48 hours after weaning + 0.25mg of GnRH (FERTAGIL® , INTERVET) IM, 72h after PMSG; group 3- received 1000 IU of PMSG 48 hours after weaning + 500 IU of hCG (PROFASI® , SERONO) IM, 72h after PMSG. Time of ovulation was monitored by transrectal ultrasound. Synchronization of ovulation rates (ovulated within 48 hours after the treatment with hCG or GnRH) in groups 2 and 3 were higher (94.7%; P< 0.01) than in group 1 (40%). Sows from groups 2 and 3 showed a tendency of higher pregnancy rate than those from group 1 (95% vs. 70%; P<0.10), but similar weaning-to-estrus interval (96.5± 3.0h vs. 130.2± 31.4h) and similar litter size (10.9± 0.8 vs. 12.0± 0.9), respectively. The data show that the protocols used in the current study were efficient in synchronize time of ovulation and render possible the use of AI at a predetermined moment