Single Strain Probiotic Bifidobacteria Approach in Health and Non-Health Fields

Single strain probiotic bifidobacteria approach is promising for the future in health and non-health fields. Recent studies show that intestinal lumen microbial content and tissue microbial content are different, so the personalized microbiome approach with the 16S rRNA analysis comes to the fore with the single strain probiotic bifidobacteria (BB-12,Infantis) approach. In addition to their immune modulation effect, they have beneficial effects such as preventing pathogens from binding to the intestinal mucosa via the biofilm layer they produce, and also their electrophysical properties in various atmospheric conditions, They have the ability to be used in non-health areas such as microplastic biodegradition, nanostructures, food and agriculture fields. The availability of single strain probiotic bifidobacteria in health, ecological and food systems are signs that progress in the single strain probiotic bacteria approach will be more accurate

Sirozlu hastalarda hepatopulmoner sendrom sıklığının araştırılması

TEZ7076Tez (Uzmanlık) -- Çukurova Üniversitesi, Adana, 2008.Kaynakça (s.47-55) var.vii, 56 s. : res. ; 29 cm.Objective: The aim of this study is to investigate hepatopulmonary syndrome frequency in cirrhosis patients with ortodeoxy which could be confirmed by transthoracic echocardiography with contrast enhancement. Material and method: Ninety five (95) patients (67/28 M/F ) with the diagnosis of cirrhosis were included in the study. Mean age of the patients was 52,28±12,0 .The cirrhosis patients' functional , clinical and etiological classifications were made.Arterial blood gas samples and measurements of portabl pulse oximetry were obtained in both supin and seated position breathing room air. The suitable patients' response to the oxygen therapy with nasal canul was evaluated. Hepatopulmonary syndrome by transthoracic echocardiography with contrast enhancement was investigated in the patients with hypoxaemia (Pa,02<85 mmHg, Sa,02<%97) in seated position. The severity and stage of hepatopulmonary syndrome were evaluated by MAA lung scanning and pulmonary angiography. Thoracic HRCT was used for excluding coexistent conditions. Data was enrolled into SPSS 16 packet program for statictical analysis.Amaç: Ortodeoksisi olan sirozlu hastalarda transtorasik kontrast ekokardiyografi ile saptanan ve sirozun bir komplikasyonu olan hepatopulmoner sendromun sıklığının araştırılması. Gereç ve Yöntemler : Çalışmaya 95 karaciğer siroz hastası (67/28 E/K) alındı. Hastaların yaş ortalaması 52,28±12,0 idi.Hastaların kronik karaciğer hastalıklarının fonksiyonel, klinik evreleri ve etyolojik sınıflamaları yapıldı.Arteriyel kan gazı ve portabl pulse oksimetre ile hastaların yatar ve oturur pozisyondaki oksijenasyon durumları değerlendirildi.Uygun hastalara nazal kanülden oksijen verilerek yanıt değerlendirildi.Oturur durumda hipoksemisi olan hastalarda (Pa,02<85 mmHg, Sa,02<%97) transtorasik kontrast ekokardiyografi ile hepatopulmoner sendrom sıklığı araştırıldı.Hepatopulmoner sendrom şiddetini ve evresini değerlendirmek için hastalara teknesyum 99m ile işaretli makroagregated albumin ile akciğer perfüzyon sintigrafisi ve pulmoner anjiografi yapıldı.Hepatopulmoner sendroma eşlik edebilecek diğer komorbit durumlar için akciğerin yüksek çözünürlüklü tomografisi çekildi. Sonuçların değerlendirilmesi için veriler SPSS 16 paket programına aktarıldı.Bu çalışma Ç.Ü. Bilimsel Araştırma Projeleri Birimi Tarafından Desteklenmiştir. Proje No

Combination of oral vancomycin and intra-colonic vancomycin: Successful treatment of complicated pseudomembranous colitis in a child patient

The Clostridium difficile infection–related disease varies from mild diarrhoea to pseudomembranouscolitis. Although C. difficile infection is commonly considered to be a hospital-acquired infection, a significant number of cases are community acquired. Community-acquired C. difficile infection can exhibit itself as ileus or toxic megacolon. Severe C. difficile infection that is unresponsive to intravenous metronidazole therapy requires more aggressive medical management and even surgical intervention. We present our case results for which vancomycin was administered both orally and intra-colonically. With this method, we treated the community acquired C. difficile infection patient who was presented as ileus

subspecies engineered to produce mycosporin-like amino acids in colorectal cancer prevention

Colorectal cancer is the third most common cancer and the third leading cause of cancer-related death. The pathogensesis of colorectal cancer involves a multi-step and multi-factorial process. Disruption of the gut microbiota has been associated with gastrointestinal diseases such as colorectal cancer. The genus Bifidobacterium is considered an important component of the commensal microbiota and plays important roles in several homeostatic functions: immune, neurohormonal, and metabolic. Bifidobacterium animalis subsp. lactis is a well-documented probiotic within the species Bifidobacterium. Mycosporin-like amino acids are low molecular weight amino acids demonstrated to exert prebiotic effects and to modulate host immunity by regulating the proliferation and differentiation of intestinal epithelial cells, macrophages and lymphocytes, as well as cytokine production.Their modulation of the metabolism of the immune system and transcription factors could exert a beneficial effect on colorectal cancer. B. animalis does not produce mycosporin-like amino acids. If one could create a B. animalis –producing mycosporin-like amino acids via genetic open reading frame engineering it should exert more potent immuno-stimulatory properties and, thereby, become a potent strain-specific microbial based therapy in colorectal cancer prevention