A synchronous occurrence of urothelial carcinoma with abundant myxoid stroma and inverted papilloma of the urinary bladder

Abundant myxoid stroma rarely occurs in urothelial carcinomas, and may cause diagnostic challenges when cells with eosinophilic cytoplasm forming nests and cords in a myxoid background are seen, particularly in the absence of typical carcinomatous appearance. Microscopic examination of transurethral resection specimen of a 71-year-old male patient revealed non-cohesive oval or elongated tumor cells with eosinophilic cytoplasm arranged in cord-like filigree pattern in an abundant myxoid stroma. Immunohisto chemically the tumor was positive for cytokeratin 7, cytokeratin 20, and 34BE12. About 90 to 100% nuclear staining was observed with p63, p53, and Ki-67. A second neoplasm with a flat overlying urothelial epithelium and a complete inverted cellular growth pattern was also noted. The neoplasm exhibited less than 2% and 10% nuclear staining with Ki-67 and p53, respectively. Considering histological, histochemical, and immunohistochemical findings, a diagnosis of synchronous urothelial carcinoma with abundant myxoid stroma and inverted papilloma was made

The Histomorphogenetic Relationship between Melanocytes and Langerhans Cells in Ovarian Mature Cystic Teratomas

Amaç: Biz bu çalışmada; hücre birimleri, organizasyon ve arşitektürel yapılanmalar bakımından orijinaline yakın sayılabilecek genel anlamda bir vücut yapımı gösteren matür kistik teratomlarda epidermisteki melanositler ve Langerhans hücrelerinin varlığı, birbiri ile sayısal ilişkileri ve birlikte bulunmaları halinde embriyolojik kaynakları hakkında fikir yürütebilmeyi amaçladık. Yöntemler: Sağlık Bakanlığı İstanbul Eğitim ve Araştırma Hastanesi Patoloji Bölümü'nde 2006-2009 yılları arasında rapor edilen 45 matür kistik teratom olgusu çalışmaya alındı. İmmünohistokimyasal olarak; melanositler için Human Melanoma Black-45 (HMB-45) ve Melanoma Antigens (Melan-A) recognized by T cells-1), Langerhans hücreleri için Cluster of Differentiation 1a (CD1a) ve Langerin uygulandı. Bulgular: Langerhans hücreleri olguların %100'ünde tespit edilmekle birlikte, melanositler %88,2'sinde mevcuttu. Sonuç: Bizim çalışmamıza göre Langerhans hücrelerinin, melanositlerin köken aldığı bilinen nöral krest dışında bir kaynaktan gelişmiş olabileceği ve bu iki hücre arasında ilişki olamayabileceği sonucuna vardık.Objective: The purpose of this study was to form a view about the existence, numerical relationship and embryological origin of melanocytes and Langerhans cells when they are found together in mature cystic teratomas which have similar cell types and architectural structure to the original vertebrated body. Methods: Forty five mature cystic teratomas cases, diagnosed in the Ministry of Health İstanbul Education and Research Hospital Pathology Department between 2006-2009 were included in the study. Immunohistochemically, Human Melanoma Black-45 (HMB-45) and Melanoma Antigens (Melan-A) recognized by T cells-1) for melanocytes, Cluster of Differentiation 1a (CD1a) and Langerin for Langerhans cells were applied. Results: Although Langerhans cells were detected in 100%, melanocytes were established in 88.2% of cases. Conclusion: According to our study, Langerhans cells could derive from another source except the neural crest where melanocytes evolve and there is no relationship between them

Histologic features of spindle cell lipoma and problems in the differential diagnosis

AMAÇ: İğsi hücreli lipomun histolojik ve tipik immunhistokimyasal özelliklerini araştırmak ve diğer subkutan yerleşimli benzer histolojik ve immunhistokimyasal özellikleri olan neoplazmlarla ayırıcı tanısını yapmaktır. YÖNTEMLER: İstanbul Eğitim ve Araştırma Hastanesi patoloji laboratuarında lipom tanısı almış 3100 olguda son Dünya Sağlık Örgütü Yumuşak Doku Tümörleri Sınıflamasına göre alt tipler belirlendi ve bunların içinden 22 İHL çalışmaya alındı. 22 iğsi hücreli lipom olgusunda klinik (yaş, lokalizasyon, cinsiyet, nüks), morfolojik özellikler (tümör boyutu, alt tip, histoloji, mast hücre varlığı ve immunhistokimya), tümörlerin natürü ve ayrıcı tanı problemleri değerlendirildi. BULGULAR: 3100 lipomun alt tiplere göre dağılımı şu şekildeydi: 2864 klasik lipom (%90), 293 anjiyolipom (%9), 1 kondroid lipom (%0.03), 1 miyolipom (%0.03 ve 22 iğsi hücreli lipom (% 0.7). 22 iğsi hücreli lipomların 9’u fibröz, 3’ü miksoid, 1’i anjiyomatoid ve 9’u nonfibröz alt tipti. Erkek kadın oranı 18/4 olarak belirlendi. Olguların 2’si sırt, 3’ü omuz, 1’i ön kol, 1’i oral olup 15’ü baş-boyun bölgesinde lokalize idi. Ortalama tümör çapı 3.2 cm olup en büyük ve küçük çaplar 1 cm ve 6.1 cm olarak ölçüldü. Fibromatöz alt tipte belirgin olmak üzere Toluidin blue boyasıyla her olguda mast hücresi mevcuttu. Mast hücre sayısı on büyük büyütme alanında ortalama 23 olarak bulundu. S–100 tüm iğsi hücrelerde negatif ve yağ hücrelerinde pozitif olarak tespit edildi. CD34 olguların 21’inde pozitif, 1’inde negatif bulundu. Hiçbir olguda nüks izlenmedi. SONUÇ: İğsi hücreli lipom, subkutan lokalizasyonlu, iyi sınırlı, iğsi hücreli komponenti CD34 pozitif, mast hücrelerinden zengin bir tümör olup birçok subkutan tümörle ayırıcı tanı yapılmasını gerektiren nadir bir antitedir.OBJECTIVE: To investigate the histologic and immunohistochemical properties of spindle cell lipoma and to make its differential diagnosis from other subcutaneous neoplasms having similar histologic or immunohistochemical charecteristics. METHODS: 3100 cases of lipoma diagnosed in the Pathology Laboratory of İstanbul Educational and Research Hospital were reclassified according to the recent classification of Soft Tissue Tumors by World Health Organization and 22 spindl cell lipomas were included in the study. Clinical (age, gender, location, and recurrences) and morphologic features (tumor size, subtype, histology, presence of mast cells, and immunohistochemistry) as well as tumor nature and problems in differential diagnosis were evaluated. RESULTS: The histologic subtypes of 3100 lipomas were as follows: 2864 classical lipoma (90 %), 293 angiolipoma (9 %), 1 chondroid lipoma (0.03 %), 1 myolipoma (0.03 %) and 22 spindl cell lipoma (% 0.7). Of the 22 spindl cell lipoma 9 were fibrous, 3 myxoid, 1 angiomatoid and 9 non-fibrous subtypes. Male to female ratio was 18: 4. 2 cases were localized in dorsal region, 3 in shoulders, 1 in forearm, 1 in oral cavity and 15 in head and neck. Average tumor diameter was 3.2 cm ranging from 1 to 6.1 cm. Mast cells highligtened by Toluidin blue were seen in all cases, most prominently in the fibrous subtype. Mast cell count in 10 high power field was 23. S–100 was negative in all spindle cells while lipomcytes were positive. CD34 was positive in 21 cases and negative in 1 case. No case recurred. CONCLUSION: Spindl cell lipoma is a subcutaneous, well-circumscribed tumor rich in mast cells with a CD34+ spindle cell component and is an uncommon entity requiring differential diagnosis with several subcutaneous tumors

The Histomorphogenetic Relationship between Melanocytes and Langerhans Cells in Ovarian Mature Cystic Teratomas

Objective: The purpose of this study was to form a view about the existence, numerical relationship and embryological origin of melanocytes and Langerhans cells when they are found together in mature cystic teratomas which have similar cell types and architectural structure to the original vertebrated body. Methods: Forty five mature cystic teratomas cases, diagnosed in the Ministry of Health Istanbul Education and Research Hospital Pathology Department between 2006-2009 were included in the study. Immunohistochemically, Human Melanoma Black-45 (HMB-45) and Melanoma Antigens (Melan-A) recognized by T cells-1) for melanocytes, Cluster of Differentiation 1a (CD1a) and Langerin for Langerhans cells were applied. Results: Although Langerhans cells were detected in 100%, melanocytes were established in 88.2% of cases. Conclusion: According to our study, Langerhans cells could derive from another source except the neural crest where melanocytes evolve and there is no relationship between them

The Histomorphogenetic Relationship between Melanocytes and Langerhans Cells in Ovarian Mature Cystic Teratomas

Objective: The purpose of this study was to form a view about the existence, numerical relationship and embryological origin of melanocytes and Langerhans cells when they are found together in mature cystic teratomas which have similar cell types and architectural structure to the original vertebrated body. Methods: Forty five mature cystic teratomas cases, diagnosed in the Ministry of Health Istanbul Education and Research Hospital Pathology Department between 2006-2009 were included in the study. Immunohistochemically, Human Melanoma Black-45 (HMB-45) and Melanoma Antigens (Melan-A) recognized by T cells-1) for melanocytes, Cluster of Differentiation 1a (CD1a) and Langerin for Langerhans cells were applied. Results: Although Langerhans cells were detected in 100%, melanocytes were established in 88.2% of cases. Conclusion: According to our study, Langerhans cells could derive from another source except the neural crest where melanocytes evolve and there is no relationship between them