Bir Bomba Fabrikası Çalışanlarindaki Total Kolesterol Düzeyleri ile Depresif Durum Arasındaki İlişki

Aim There is significant amount of evidence linking low cholesterol levels to aggressive behaviors, depression and suicide. Material and Methods The objective of this study was to determine whether a correlation exists between serum total cholesterol levels and depressive state in 77 male workers labouring in a bomb plant and in 80 male office personnel. Depressive statuses of the subjects were determined by using Beck Depression Rating Scale and Zung Self-Rating Depression Scale. Results Depression scores were significantly higher and total cholesterol levels were significantly lower in bomb factory workers than that of office staff. In both types of workers, cholesterol levels were negatively correlated with depression scores according to both Beck and Zung scales. Total cholesterol levels of bomb factory workers with moderate and severe depression symptoms were significantly lower when compared with normal and mild depression. Conclusion These data indicate that lowserum total cholesterol level is associated with increased risk of depressive state

Anaerobik Glikoliz HL-60 Akut Promyelositik Lösemi Hücrelerinde Enerjinin Oluşumu için Temel Yoldur

Aim In physiological conditions, normal cells use mainly the glycolytic aerobic pathway to provide energy. However, most cancer cells utilize anaerobic glycolytic way for energy generation. Aim of this study was to investigate the carbohydrate metabolic pathways of HL-60 acute promyelocytic leukemia cells for energy production. Material and Methods Leukemia cells as well as normal leukocytes were incubated with radiolabelled glucose in aerobic and anaerobic conditions and glycogen consumptions and the ratios of radiolabelled glucose catabolized into CO2 or lactate, that is, the rates of aerobic or anaerobic glycolysis, were determined. Results The glycogen consumption was significantly higher in aerobic leukemia cell culture than normal leukocyte culture (p<0.01). The rate of anaerobic glycolysis was 93.8% in leukemia cells in aerobic conditions and it increased to 96.6% while utilization of glycogen increased by 7.31% in anaerobic conditions. Conclusion In conclusion, principally anaerobic glycolysis is effective for energy generation in HL-60 promyelocytic leukemia cells. This result may be important for the development of new therapeutic approaches in the treatment of promyelocytic leukemia, requiring further comprehensive studies

A research on the level of urine neopterin to see if it may provide a vital clue for a provisional diagnosis of breast cancer in menopausal women

WOS: 000223446200012PubMed: 15283936Objectives: To search the relation between the level of neopterin in urine and breast cancer developed in menopause. Methods: In this study, urine samples were collected from randomly selected menopausal and post-menopausal women who attended hospital, and from a control group. The participants were classified into two representative sample groups and a control group; 1st group: 30 menopausal and post-menopausal women whose ages varied from 45 to 80 and who suffered from breast cancer but had no viral infections, chronic inflammatory diseases and smoking habit; 2nd group: 30 menopausal and post-menopausal women aged between 48 and 63 with no complaint of any type of tumors, viral infections or chronic inflammatory diseases and with no smoking habit; 3rd group: A control group with 20 women aged between 20 and 28, who did not take part in sexual activities yet, and had no complaint of any type of tumors, viral infections or chronic inflammatory diseases and had no smoking habit. Urinalysis was carried out for each sample in order to measure the level of neopterin. Results: The mathematical results of neopterin levels for the groups showed that group I was significantly higher than group 11 and III (P = 0.0001); group H was significantly higher than group III (P = 0.003). Conclusions: If the results of this study were not confounded by another factor, then can we deduce that this relationship can be used as a risk factor that should warrent further investigation of breast cancer during the care and treatment of menopausal women. (C) 2004 Elsevier Ireland Ltd. All rights reserved

Fungal growth inside saline-filled implants and the role of injection ports in fungal translocation: In vitro study

WOS: 000224396700022PubMed: 15457030Infection is a serious complication of breast. augmentation and tissue expansion with inflatable devices. Several reports have shown that fungi may be able to survive, colonize, and even cause infection in saline-filled devices. The mechanism of how them, penetrate, spread, and colonize inside the inflatable implants is not exactly understood. The authors assessed both the expander membrane and the port in terms of leakage and penetration of Candida albicans and Aspergillus niger in an in vitro model. Thirty saline-filled expanders connected to the injection port were placed in sterile containers filled with tryptic soy broth culture medium to simulate the clinical situation in phases I and II. Intactand multipunctured ports were used in the first and second phases of the study, respectivelv. Either the container or the implant was inoculated with one of these fungi, and six implants in containers without fungal inoculation served as controls. As a third phase, intraluminal survival of fungi was investigated in saline-filled containers (n = 12) in 21 clays. The silicone membrane, with its intact connecting tube and port, was impermeable to these fungi, whereas both fungi were able to diffuse inside-out or outside-in through the punctured ports. C. albicans did not Survive beyond 18 days in saline, whereas A. niger continued to multiply at day 21. Chemical analyses of the implant fluids revealed that the contents of the culture medium diffused into the implants in phases I and II. The data show that an intact. silicone membrane is impermeable to fungi, and punctured ports allow translocation of fungi into the implants. Fungi can grow and reproduce in a saline-only environment, and their survival periods differ among the species. Furthermore, their Survival maybe enhanced by the influx of substances through the implant shell

Alteration of tumor metabolism after radiotherapy.

40th Annual Meeting of the American-Society-of-Clinical-Oncology -- JUN 05-08, 2004 -- New Orleans, LAWOS: 000223512403478…Amer Soc Clin Onco

Fasciola hepatica miracidia are dependent on respiration and endogenous glycogen degradation for their energy generation

PubMed: 11272647It is generally accepted that free-living stages of parasitic helminths are dependent on aerobic degradation of endogenous energy sources for their energy generation. This concept, however, is not the result of extensive experimental evidence, but originated mainly intuitively as oxygen is widely available in their habitat and these stages generally have a small size. Schistosoma mansoni, the sole parasitic helminth whose energy metabolism has been studied throughout its life-cycle indeed has aerobically functioning free-living stages. However, large differences exist in energy metabolism between adult stages of distinct parasitic helminths, and caution should be taken in predicting that all free-living stages of all parasitic helminths have the same, aerobic energy metabolism. Hence, this report studied the energy metabolism of Fasciola hepatica miracidia and demonstrated that F. hepatica miracidia are also dependent on aerobic degradation of their endogenous glycogen stores by glycolysis and on Krebs cycle activity for energy generation. However, in contrast to S. mansoni, F. hepatica miracidia cannot function anaerobically, as inhibition of the respiratory chain blocked motility and carbohydrate degradation, and finally resulted in death of the miracidia. Therefore, this report demonstrated that differences exist between miracidia of distinct species, in pre-adaptation of their energy metabolism to the occasional hypoxic conditions within their next host

Primary bilateral spermatocele

Batislam, Ertan/0000-0002-7493-4573WOS: 000180479800015PubMed: 12534930We report the case of a bilateral, relatively huge, multilocular spermatocele diagnosed at sonography with surgical and pathological correlation. A 45-year-old man presented with bilateral, large inguinoscrotal masses. He had had the masses since his adolescence and their size gradually increased in the past 5 years. Scrotal ultrasonographic examination showed cystic fluid masses that were 65 x 45 x 50 mm in size on the right and 55 x 45 x 40 mm in size on the left side in multilocular spaces. The cystic masses were excised via bilateral inguinoscrotal incision staying near the body of the epididymis. The patient had fathered three children with no fertility problem

The detection of urinal neopterin concentration increases the efficiency of cervical smear in the diagnosis of cervical cancer.

PubMed: 15446363OBJECTIVE: To investigate the importance of urinal neopterin detection carried out together with cervical smear test, in the diagnosis of cervical cancer. METHODS: In this study, urine samples were collected from randomly selected vulvovaginitis' women who attended hospital and an independent control group. The participants were classified into two representative sample groups and a control group; 1st Group: 35 women with vulvovaginitis, whose ages varied from 21 to 42 and who had no viral infections, and chronic inflammatory diseases and no smoking habit, but had complaints of vaginal discharge. 2nd Group: 25 women aged between 38-52 with no smoking habit, who applied to hospital with complaints of vaginal discharge, and cervical cancer was diagnosed for them as the result of biopsy, but no associated treatment of cancer had been carried out yet. 3rd Group: A control group with 30 women who aged between 20 and 28, with no smoking habit, and who had not taken part in sexual activities yet. They had no complaints from any type of tumors, and viral infections or chronic inflammatory diseases. Urinalysis was carried out for each group member's urine sample in order to measure the level of neopterin. Neopterin was estimated by HPLC. Some statistical analyses were done by SPSS Windows 10.0 and were analyzed by Oneway Annova test. (p=0.000) Meaningful differences between the groups were determined by Post Hoc Tukey Test. RESULTS: The mathematical results of neopterin levels for the groups revealed that the level of Group 1 was significantly higher than the level of Group 3 (p= 0.0001). When the levels of Group 1 and 2 were compared with each other, a significant difference was determined (p=0.004). Also, the difference in the levels of Group 2 and 3 was found to be significant (p=0.0001). CONCLUSIONS: If the results of this study were not confounded by another factor, then we can deduce that an increment in the level of neopterin may be considered as a risk factor that should warrant further investigation of cervical cancer. Then, the detection of urinal neopterin level as a noninvasive test done together with cervical smear can increase the sensitivity of smear test

Urinary neopterin levels in the different stages of pregnancy

Yucel, Aykan/0000-0002-5888-692XWOS: 000228257200011PubMed: 15692214Background/Aims: Neopterin is a biochemical marker of activated cell-mediated immune response which increases in pathological conditions associated with cellular immune activation as well as in pregnancy where cellular immune response is predominant. The aim of this study was to determine the urinary neopterin level in each trimester of pregnancy and to determine if it can be used as a marker. Methods: 104 healthy pregnant women ( mean age 22.10 +/- 4.39 years; 36 in the first, 30 in the second and 38 in the third trimester) and 16 nonpregnant healthy women ( mean age 20.94 +/- 4.48 year) were included. Results: The mean urinary neopterin concentration of all pregnant women was higher than that of non-pregnant women (166.4 +/- 31.7 and 103.1 +/- 27.5 mu mol/mol creatinine respectively, p < 0.01). The mean urinary neopterin levels in each trimester and nonpregnant women were 139.8 +/- 49.6, 131 +/- 40.2, 227.9 +/- 86.5 and 103.1 +/- 27.5 mu mol/mol creatinine, respectively. Urinary neopterin levels were not significantly different between non-pregnant, first and second trimester groups, but were significantly higher in the third trimester than each of these groups ( p < 0.05, p < 0.01 and p < 0.01 respectively). Conclusion: Urinary neopterin levels increase significantly in the third trimester probably due to more predominated cellular immunity. The pathologies causing cellular immune activation, especially in the first two trimesters can be predicted with urinary neopterin levels. Copyright (C) 2005 S. Karger AG, Basel