Molekularbiologische Charakterisierung neuartiger Enzyme aus dem Basidiomyceten Pleurotus sapidus

[no abstract

Julius Rontgen

The German born composer Julius Rontgen who settled in Amsterdam, set Jan FE Celliers' poem Komaan to music. It was not the only incidence of European composers writing music for Afrikaans poetry.pdf file containing digitised version of an article published in die Huisgenoot of 22 Oct 1937.er2014-2pm2014-

Einfluss von Feldgröße, Geometrie und der Nachbarkultur auf den Befall mit Phytophthora infestans und auf die Ertragsbildung in Kartoffelbeständen

Genetische Vielfalt und das Mikroklima innerhalb eines Feldes kann die Entwicklung von Epidemien massiv beeinflussen. In Kartoffelbeständen kann Vielfalt durch Sortenmischungen in der Reihe, alternierenden Reihen oder Streifen verschiedener Sorten (siehe Finckh et. al. 2003, dieser Band) oder im Streifenanbau abwechselnd mit anderen Kulturen erreicht werden. Beim Streifenanbau mit verschiedenen Kulturen sind eine große Anzahl Anbaufaktoren beeinflußbar wie Feldlänge und - breite, Ausrichtung zum Wind und die Art der Nachbarkulturen. Diese können, wenn sie höher als die Kartoffelbestände sind als Barrieren für Wind und Krankheitsinokulum dienen, eventuell aber auch die Bestandesfeuchte erhöhen. Niedrigere Kulturen dagegen können die Bestandesfeuchte erniedrigen. Neben diesen direkten Effekten auf den Bestand wird auch die regionale Bestandesdichte und damit die absolute Inokulumproduktion reduziert. Neben Einflüssen auf den Epidemieverlauf können aber auch Konkurrenzbeziehungen verschiedener Kulturen im Feld eine massive Rolle in der Epidemieentwicklung und in der Ertragsbildung spielen

The Lived Experience of the Novice Nursing Dean: Exploring the Meaning and Significance

Deans of nursing hold a unique position for the future of the profession. Few others have the potential to bring about change within the academic setting, or hold such a strong influence on the future of nursing and on our students. Current statistics reveal multiple vacancies in nursing dean positions. Further understanding of how nursing deans begin their role, attain their identity as dean, and successfully execute this important position is needed to recruit and retain strong leaders who have the methods and skills to develop and support the advancement of the nursing profession. Understanding the process of becoming nursing dean can advance knowledge development in the profession as it applies to retention and recruitment of nurse administrators. The purpose of this phenomenological study was to describe the essence of the lived experience for those who have recently advanced to academic dean of nursing. Theories of identity, human becoming and role transition were referenced as conceptual underpinnings for the study. The interpretive phenomenological approach of van Manen guided the inquiry and data analysis. The overarching research question was: What is the lived experience of a novice nursing dean? Purposive and snowball sampling was used to select participants based on their years as dean. The sample included deans that are Registered Nurses, have the title of Dean, have not been in a dean role previously, and have been appointed in their first dean position three years or less. Seven nursing deans participated in the study, and the technique used to gather data was the recording of face-to-face interviews conducted in a semi-structured format. The interviews were transcribed and manual coding was performed on the data to create categories or themes that reflected the data. The results yielded four major themes and 16 subthemes. The overarching themes of sacrifice, lack of preparation for the role, the uniqueness of the nursing dean role, and highly political environments inform what a nursing dean experiences within the first three years of their role. The information gathered in this study provides more understanding of the complex role of novice nursing deans. It also provides new insight that will be useful as we look to recruit and retain these important leaders

A comparison of cell wall disruption techniques for the isolation of intracellular metabolites from Pleurotus and Lepista sp.

Different techniques were compared for their effectiveness in the disruption of the rigid cell walls of Basidiomycetes, Grinding under liquid nitrogen, stirred glass bead milling and enzymatic cell lysis were applied to the mycelia of Pleurotus sapidus and Lepista irina grown submerged. Each of the disruption procedures was evaluated by testing the quantity and quality of released intracellular metabolites: DNA, RNA, enzymes, and secondary metabolites. The most suitable method for nucleic acid isolation was grinding under liquid nitrogen, while bead mill homogenization was the superior technique for isolation of active enzymes. A new effective method is proposed for isolation of secondary metabolites with the aid of bead milling of fungal mycelia. © 2006 Verlag der Zeitschrift für Naturforschung

Neue Erkenntnisse zur Ertragsrelevanz der Kraut- und Knollenfäule

Zur Kontrolle der Kraut- und Knollenfäule (Phytophthora infestans) im ökologischen Kartoffelanbau wurde in einem EU-Forschungsprojekt ein Systemansatz entwickelt. Ziel war unter anderem, den Einsatz von Kupferfungiziden zu reduzieren

Conditioned neuroendocrine and cardiovascular stress responsiveness accompanying behavioral passivity and activity in aged and in young rats

Mean arterial pressure (MAP), heart rate (HR), plasma epinephrine (E), plasma norepinephrine (NE), and plasma corticosterone (CORT) were measured in 3-month- and 24-month-old male Wistar rats exposed to a conditioned emotional stress response (CER) paradigm and a conditioned defensive burying (CDB) paradigm. In the CER situation blood samples were taken during reexposure to the training environment one day after a single inescapable footshock (0.6 mA, AC for 3 s) had been administered. In the CER paradigm the young rats displayed passive behavior (immobility) accompanied by an increase in plasma levels of CORT and E. whereas both the control and conditioned animals showed increased NE responses. Previously shocked aged rats exhibited an attenuated plasma NE response, whereas levels of E remained elevated to a greater extent. Aged animals showed elevated basal levels of CORT one day after footshock administration. Stress-induced immobility was preserved in the aged rats. These animals had an increase in basal MAP values and a decrease in basal HR values compared to young ones.In the CDB paradigm, rats were exposed to a nonelectrified probe 1 day after the repeated shock (2 mA/contact) procedure. Young rats displayed defensive burying accompanied by increments in MAP, HR, CORT. and NE. The aged animals showed similar hormonal, autonomic, and behavioral stress responses.Thus, the age-related alterations in neuroendocrine and autonomic response patterns are apparent in stressed animals during behavioral passivity in absence of control (CER) rather than during active control (defensive burying).</p