Exploration du récit de vie d’adolescents

peer reviewedThe present paper follows on from the research we presented in previous JADT (Boulard, Poudat, Gauthier 2012). We still focus on the development of narrative competence (Habermas et Bluck 2000) in children and adolescents. Although children develop narrative skills, life narratives only emerge in adolescence. On the basis of a corpus made of spontaneous oral speech, we had empirically demonstrated that pre-adolescents aged of 12 had developed stabilized narrative skills enabling them to produce life narratives. Here, we propose a first exploration of the overall structure of adolescent life stories, thanks to a corpus of 268 oral self narratives completed with a questionnaire

Des mots pour se dire: Développement de la fonction narrative chez l'enfant.

peer reviewedThe present study is based on a corpus made of spontaneous oral speech: 136 children aged from 3 to 13 have been recorded in three different environments that are favorable to the production of self-narratives. Using linguistic annotations and tried and tested text statistics methods, we have been able to show different aspects of the development of narratives and self-narratives in children speeches, including the impact of age and environment on the construction of narratives. The results we obtained are significant, and we hope we succeeded in demonstrating how relevant text statistics methods are when applied to clinical psychology

Récits de vie d’adolescents. Humeur dépressive et figures parentales

La présente étude se propose d’explorer d’explorer les liens entre l’humeur dépressive et le langage sur la base d’un corpus de récits de vie oraux spontanés produits par 206 adolescents âgés de 12 à 18 ans. On se concentrera plus spécifiquement sur les représentations des figures d’attachement parentales dans les discours, premiers paramètres à jouer un rôle déterminant pour spécifier les différences d’humeur de l’adolescent, en mobilisant des méthodes issues de l’analyse de données textuelles et de la linguistique de corpus

Cooccurrences des personnes dans le discours de l’enfant : une approche statistique de la construction de l’identité

National audienc

The development of children's and preadolescent's narratives

The aim of this study is to explore the development of children’s narratives in three different contexts that are favorable to the production of (self-)narratives: (i) at home with parent(s), (ii) at school with peers and (iii) with a researcher in psychology. 136 children aged from 3 to 13 have been recorded 50 minutes in each situation. Once transcribed, the data were morphosyntactically annotated and processed using multivariate exploratory techniques, including Correspondence Analysis, Principal Component and Classification Analysis. The results we obtained first demonstrated that contextual indicators were more significant than age in narrative production: conversations with parents were significantly more interactive whereas interactions between children turned out to be more regressive (i.e. simplified vocabulary, demonstrative pronouns). The situation with the psychologist was the one leading to the more elaborate narratives, as children seem to show greater language control with unfamiliar adults. However, age naturally impacts the linguistic complexity of narratives, both on the lexical and morphosyntactic levels. The results we obtained enabled us to observe a gradation in the complexity of textual productions, setting the roots of the future self-narratives the subjects will be able to develop later, during adolescence. Thus, the findings show different and significant aspects of the development of narratives and self-narratives in children’s speech, considering age and context. Last but not least, they also suggest the relevance of the application of text statistics methods to clinical psychology

Cooccurrences des personnes dans le discours de l'enfant: une approche statistique de la consturction de l'identité

peer reviewedÀ partir de l’enregistrement de discours spontanés d’enfants âgés de 3 à 8 ans dans trois conditions (psychologue, groupe, parents), l’objectif de cette recherche sera d’appréhender le développement du rapport que l’enfant entretient avec lui-même et les autres en observant la distribution et les utilisations particulières des formes personnelles chez l’enfant. Pour qualifier ces emplois, nous examinerons le détail des cooccurrents des pronoms pour chaque catégorie considérée. La situation de groupe s’étant avérée particulièrement concluante, nous lui consacrerons la dernière partie de nos explorations en nous concentrant sur l’asymétrie cooccurrentielle du couple moi-je dans la continuité de nos travaux autour de l’asymétrie de la relation coocurrentielle (Luong et al. 2010) en insistant sur leurs résonances dans le développement de l’identité chez l’enfant

Purification and protein composition of oil bodies from Brassica napus seeds

Seed oil bodies are intracellular particles to store lipids as food reserves in oleaginous plants. Description of oil body-associated proteins of Arabidopsis thaliana has been recently reported whereas only few data are available in the case of rapeseed. Oil bodies have been prepared from two double-low varieties of Brassica napus seeds, a standard variety (Explus) and an oleic variety (Cabriolet). Oil bodies have been purified using floatation technique in the successive presence of high salt concentration, detergent or urea in order to remove non-specifically trapped proteins. The integrity of the oil bodies has been verified and their size estimated. Their protein and fatty acid contents have been determined. The proteins composing these organelles were extracted, separated by denaturing gel electrophoresis, digested by trypsin and their peptides were subsequently analyzed by liquid chromatography-tandem mass spectrometry. Protein identification was performed using Arabidopsis thaliana protein sequence database and a collection of Expressed Sequence Tag (EST) of Brassica napus generated from the framework of the French plant genomics programme “Genoplante”. This led to the identification of a limited number of proteins: eight oleosins showing a high similarity each other and representing up to 75% of oil body proteins, a 11 β hydroxysteroid dehydrogenase-like protein highly homologous to the same protein from A. thaliana, and only few contaminating proteins associated with myrosinase activity

Purification and protein composition of oil bodies from Brassica napus seeds

Seed oil bodies are intracellular particles to store lipids as food reserves in oleaginous plants. Description of oil body-associated proteins of Arabidopsis thaliana has been recently reported whereas only few data are available in the case of rapeseed. Oil bodies have been prepared from two double-low varieties of Brassica napus seeds, a standard variety (Explus) and an oleic variety (Cabriolet). Oil bodies have been purified using floatation technique in the successive presence of high salt concentration, detergent or urea in order to remove non-specifically trapped proteins. The integrity of the oil bodies has been verified and their size estimated. Their protein and fatty acid contents have been determined. The proteins composing these organelles were extracted, separated by denaturing gel electrophoresis, digested by trypsin and their peptides were subsequently analyzed by liquid chromatography-tandem mass spectrometry. Protein identification was performed using Arabidopsis thaliana protein sequence database and a collection of Expressed Sequence Tag (EST) of Brassica napus generated from the framework of the French plant genomics programme “Genoplante”. This led to the identification of a limited number of proteins: eight oleosins showing a high similarity each other and representing up to 75% of oil body proteins, a 11 β hydroxysteroid dehydrogenase-like protein highly homologous to the same protein from A. thaliana, and only few contaminating proteins associated with myrosinase activity

Purification and protein composition of oil bodies from Brassica napus seeds

Seed oil bodies are intracellular particles to store lipids as food reserves in oleaginous plants. Description of oil body-associated proteins of Arabidopsis thaliana has been recently reported whereas only few data are available in the case of rapeseed. Oil bodies have been prepared from two double-low varieties of Brassica napus seeds, a standard variety (Explus) and an oleic variety (Cabriolet). Oil bodies have been purified using floatation technique in the successive presence of high salt concentration, detergent or urea in order to remove non-specifically trapped proteins. The integrity of the oil bodies has been verified and their size estimated. Their protein and fatty acid contents have been determined. The proteins composing these organelles were extracted, separated by denaturing gel electrophoresis, digested by trypsin and their peptides were subsequently analyzed by liquid chromatography-tandem mass spectrometry. Protein identification was performed using Arabidopsis thaliana protein sequence database and a collection of Expressed Sequence Tag (EST) of Brassica napus generated from the framework of the French plant genomics programme “Genoplante”. This led to the identification of a limited number of proteins: eight oleosins showing a high similarity each other and representing up to 75% of oil body proteins, a 11 β hydroxysteroid dehydrogenase-like protein highly homologous to the same protein from A. thaliana, and only few contaminating proteins associated with myrosinase activity

Selective One-Step Extraction of Arabidopsis thaliana Seed Oleosins Using Organic Solvents

International audienceOleosins are hydrophobic proteins from oleaginous seeds, surrounding and stabilizing oil bodies. They are known to display interesting interfacial properties. Specific sera were raised against four different A. thaliana oleosins and used in dot-blot assays for oleosin quantification. These assays were used to set up extraction of oleosins from A. thaliana seeds. One mixture of chloroform/methanol gave optimal oleosin extraction. Extracted proteins represented 9% of seed proteins and were identified by immunoblot and proteomic analyses. Oleosins accounted for 79% of the extracted proteins. This simple one-step procedure allows selective extraction and concentration of oleosins from seeds without tedious oil body purification. Oleosin extract was indeed used to demonstrate the presence of the rare oleosin S5 in mature seeds. Moreover, this method will be useful to investigate the potential use of oleosins as emulsifier and to question their possible allergenicity