Effects of hematopoietic growth factors on purified bone marrow progenitor cells

We have used highly enriched hematopoietic progenitor cells and in-vitro culture to examine the following questions: 1. The effects of recombinant lL-3 and GM-CSF on proliferation and differentiation of enriched hematopoietic progenitor cells have not been clearly defined: - how do IL~3 and GM~CSF compare with respect to number and types of colonies induced? -to what extent do accessory cells influence colony formation induced by IL-3 and GM~CSF? {chapters 2 and 3) 2. The effects of recombinant G-CSF, M~CSF and IL-6 on enriched hematopoietic progenitor cells in connection with IL-3 and GM~CSF have not been fully elucidated: ~ what is the role of synergistic effects between G~CSF, M-CSF and IL~6 on the one hand and IL~3 and GM~CSF on the other hand, on the proliferation and differentiation of colony forming cells? (chapters 4, 5 and 6) 3. Consistent results on the effects of ll-1 on proliferation of immature bone marrow cells are lacking: ~does ll-1 directly induce proliferation? -what is the role of GM~CSF and TNF as mediators of the IL-1 effect? (chapter 7) 2

Prognostic factors in renal-cell carcinoma: Immunohistochemical detection of p53 protein versus clinico-pathological parameters

Immunoreactivity forp53 protein was assessed in 100 cases of primary renal-cell carcinoma (RCC). The results were correlated with clinical survival data (follow-up 24 to 84 months: mean: 39 months) and with clinico-pathological parameters, including nuclear grade, tumour stage, cell type, tumour architecture and tumour diameter. In all, 32% of the tumours were p53-positive; there was no difference in survival between p53-positive and -negative cases. Similarly, p53 expression did not correlate with any of the clinico-pathological parameters mentioned. Nuclear grade (grade 1 + 2 vs. grade 3 + 4) had a striking impact on prognosis and so, to a lesser extent, did tumour stage and the occurrence of a spindle-cell component. The immunohistochemical detection of p53 in RCC is not of prognostic value. The estimation of nuclear grade, however is a major predictor of prognosis