Effects of hematopoietic growth factors on purified bone marrow progenitor cells

Abstract

We have used highly enriched hematopoietic progenitor cells and in-vitro culture to examine the following questions: 1. The effects of recombinant lL-3 and GM-CSF on proliferation and differentiation of enriched hematopoietic progenitor cells have not been clearly defined: - how do IL~3 and GM~CSF compare with respect to number and types of colonies induced? -to what extent do accessory cells influence colony formation induced by IL-3 and GM~CSF? {chapters 2 and 3) 2. The effects of recombinant G-CSF, M~CSF and IL-6 on enriched hematopoietic progenitor cells in connection with IL-3 and GM~CSF have not been fully elucidated: ~ what is the role of synergistic effects between G~CSF, M-CSF and IL~6 on the one hand and IL~3 and GM~CSF on the other hand, on the proliferation and differentiation of colony forming cells? (chapters 4, 5 and 6) 3. Consistent results on the effects of ll-1 on proliferation of immature bone marrow cells are lacking: ~does ll-1 directly induce proliferation? -what is the role of GM~CSF and TNF as mediators of the IL-1 effect? (chapter 7) 2