Element signature analysis: its validation as a tool for geographic authentication of the origin of dried beef and poultry meat

Element concentrations of 56 poultry meat and 53 dried beef samples were determined and statistically analyzed using analysis of variance and linear discriminant analysis (LDA) to identify the single or combination of elements with the highest potential to determine the geographic origin. In order to validate the applicability of this technique, the results were additionally combined with data from an earlier assessment including 25 poultry meat and 23 dried beef samples. Validation was performed by estimating the origin of the first samples based on the data of the second, larger, dataset. Elements significantly discriminating among countries were As, Na, Rb, Se, Sr, and Tl for poultry meat and As, B, Ba, Ca, Cd, Cu, Dy, Er, Fe, Li, Mn, Pd, Rb, Se, Sr, Te, Tl, U, and V for dried beef out of about 50 elements each. The LDA gave mean correct classification rates of 77 and 79% for poultry meat and dried beef, respectively. Validation allowed identifying some, but not all, origins. For a higher discriminative power, this method should be combined with other ways of authenticatio

Quantitative analysis of physical and chemical measurands in honey by mid-infrared spectrometry

Fourier transform infrared spectroscopy (FT-IR) was used to determine 20 different measurands in honey. The reference values for 144 honey samples of different botanical origin were determined by classical physical and chemical methods. Partial least squares regression was used to develop the calibration models for the measurands studied. They were validated using independent samples and proved satisfying accuracies for the determination of water (R 2=0.99), glucose (0.94), fructose (0.84), sucrose (0.91), melezitose (0.98) and monosaccharide content (0.82) as well as fructose/glucose ratio (0.98), glucose/water ratio (0.94), electrical conductivity (0.98), pH-value (0.87) and free acidity (0.96). The prediction accuracy for hydroxymethylfurfural, proline and the minor sugars maltose, turanose, erlose, trehalose, isomaltose and kojibiose was rather poor. The results demonstrate that mid-infrared spectrometry is a valuable, rapid and non-destructive tool for the quantitative analysis of the most important measurands in hone

Indications for the applicability of element signature analysis for the determination of the geographic origin of dried beef and poultry meat

In order to determine the geographic origin of poultry and dried beef, concentrations of a total of 72 different elements (occasionally represented with several isotopes) were analyzed by inductively coupled plasma high resolution mass spectrometry (ICP-HRMS). Additionally, gross chemical composition (GCC) was analyzed. The 25 poultry breast filets samples originated from Switzerland, France, Germany, Hungary, Brazil, and Thailand, and the 23 dried beef samples, made from M. biceps femoris and M. semitendinosus, were produced in Switzerland, Austria, Australia, United States, and Canada out of raw meat originating either from these or from other countries. A total of 66 and 46 of the elements and isotopes followed were detected in beef and poultry, respectively. For statistical analyses, only the most abundant isotopes per element were used. For both poultry meat and dried beef, a differentiation of the origins was possible using those elements, which were significantly different across countries (As, Na, Rb, and Tl in poultry; B, Ca, Cd, Cu, Dy, Eu, Ga, Li, Ni, Pd, Rb, Sr, Te, Tl, Tm, V, Yb, and Zn in beef). No sufficient differentiation between origins was possible with GCC. Further studies have to confirm the suitability of this approach for meat authentication with more sample

Quantitative determination of physical and chemical measurands in honey by near-infrared spectrometry

Fourier transform near-infrared spectroscopy (FT-NIR) was evaluated to quantitatively determine 24 different measurands in honey. The reference values of 421 honey samples of different botanical origins were determined by classical physical and chemical methods. Partial least squares regression was used to develop the calibration models for the measurands studied. These calibrations were then validated using independent samples and proved satisfying accuracies for the determination of water (standard error of prediction: 0.3g/100g), glucose (1.3g/100g), fructose (1.6g/100g), sucrose (0.4g/100g), total monosaccharide content (2.6g/100g) as well as fructose/glucose ratio (0.09) and glucose/water ratio (0.12). The prediction accuracy for hydroxymethylfurfural, proline, pH-value, electrical conductivity, free acidity and the minor sugars maltose, turanose, nigerose, erlose, trehalose, isomaltose, kojibiose, melezitose, raffinose, gentiobiose, melibiose, maltotriose was poor and unreliable. The results demonstrate that near-infrared spectrometry is a valuable, rapid and non-destructive tool for the quantitative analysis of some measurands related to the main components in hone

Tracing the geographic origin of poultry meat and dried beef with oxygen and strontium isotope ratios

Two promising isotope ratios, the δ18O of the water fraction, as extracted by azeotropic distillation, and the 87Sr/86Sr of the ash fraction were tested for their applicability to determine the geographic origin. In two sampling phases, in total 78 fresh poultry breast meat and 72 dried beef samples, independent from each other and originating from different countries, were analysed. The δ18O was measured with isotope ratio mass spectrometry and the isotope abundance ratio of 87Sr/86Sr with a multicollector inductively coupled plasma mass spectrometer. With δ18O it was possible to distinguish (p<0.001) poultry and dried beef samples according to their country of origin. The beef data suggests that the procedure of processing is of only low additional influence on δ18O and, if so, it seems to reduce the initial between-country differences. The 87Sr/86Sr ratio did not give sufficient indications for differentiation by geographic origin in either poultry meat or dried beef in the smaller, first phase, data set and was therefore not further teste

Utilisation of mid-infrared spectroscopy for determination of the geographic origin of Gruyere PDO and L'Etivaz PDO Swiss cheeses

Correspondance auteur: Karoui R. E-mail: [email protected] audienceThe potential of mid-infrared spectroscopy (MIR), using an attenuated total reflectance (ATR) cell, was evaluated for the authentication of 25 Gruyere PDO and L'Etivaz PDO cheeses produced at different altitudes in Switzerland. In order to test the ability of MIR to authenticate the investigated cheeses, chemometric tools, such as principal component analysis (PCA) and factorial discriminant analysis (FDA), were applied to the three spectral regions of the MIR (e.g. 3000-2800 cm(-1), 1700-1500 cm(-1), and 1500-900 cm(-1)). By applying the FDA to the first 10 principal components (PCs) of the PCA applied to each spectral regions, the best rate of correct classification was obtained in the 3000-2800 cm(-1) and 1500-900 cm(-1) spectral regions, since 90.5% and 90.9% were achieved, respectively. It can be concluded that these two spectral regions could be considered as valuable tools for the determination of the geographical origin of the investigated cheese