The Elastic Behaviour of Sintered Metallic Fibre Networks: A Finite Element Study by Beam Theory.

This is the final version of the article. It first appeared from PLOS via http://dx.doi.org/10.1371/journal.pone.0143011BACKGROUND: The finite element method has complimented research in the field of network mechanics in the past years in numerous studies about various materials. Numerical predictions and the planning efficiency of experimental procedures are two of the motivational aspects for these numerical studies. The widespread availability of high performance computing facilities has been the enabler for the simulation of sufficiently large systems. OBJECTIVES AND MOTIVATION: In the present study, finite element models were built for sintered, metallic fibre networks and validated by previously published experimental stiffness measurements. The validated models were the basis for predictions about so far unknown properties. MATERIALS AND METHODS: The finite element models were built by transferring previously published skeletons of fibre networks into finite element models. Beam theory was applied as simplification method. RESULTS AND CONCLUSIONS: The obtained material stiffness isn't a constant but rather a function of variables such as sample size and boundary conditions. Beam theory offers an efficient finite element method for the simulated fibre networks. The experimental results can be approximated by the simulated systems. Two worthwhile aspects for future work will be the influence of size and shape and the mechanical interaction with matrix materials.This research was supported by the European Research Council Grant No 240446 (http://erc.europa.eu/)

Mechanical bone growth stimulation by magnetic fibre networks obtained through a competent finite element technique.

Fibre networks combined with a matrix material in their void phase make the design of novel and smart composite materials possible. Their application is of great interest in the field of advanced paper or as bioactive tissue engineering scaffolds. In the present study, we analyse the mechanical interaction between metallic fibre networks under magnetic actuation and a matrix material. Experimentally validated FE models are combined for that purpose in one joint simulation. High performance computing facilities are used. The resulting strain in the composite's matrix is not uniform across the sample volume. Instead we show that boundary conditions and proximity to the fibre structure strongly influence the local strain magnitude. An analytical model of local strain magnitude is derived. The strain magnitude of 0.001 which is of particular interest for bone growth stimulation is achievable by this assembly. In light of these findings, the investigated composite structure is suitable for creating and for regulating contactless a stress field which is to be imposed on the matrix material. Topics for future research will be the advanced modelling of the biological components and the potential medical utilisation

Nano-CT scans in the optimisation of purposeful experimental procedures: A study on metallic fibre networks.

Motive Metallic fibre networks and their mechanical behaviour are only insufficiently understood. In this particular field of research, the use of nano-CT scans offers advanced opportunities for the optimised planning of experimental work and component design. Several novel applications will benefit from this research; in particular, tissue engineering applications where a controlled and reproducible mechanical stimulus on cells is required can make use of these components. MethodFor the present study, the geometry of metallic fibre network samples is measured and digitalised through the use of nano-CT scan protocols and adequate radiological post-processing steps. Fibre medial axes are transferred into finite element assemblies and are exposed to magnetic actuation models. Network displacement of input geometries is quantified by averaging of node displacement fields. Key resultsComplex 3D deformation fields with regions of tension, shear, and compression are obtained. Results from a previous study about matrix material deformation can be confirmed in this study for greater sample geometries. The strain magnitude is not uniform across the samples; several influencing parameters and deformation patterns are identified. A simple analytical model can be presented which quantifies the material deformation. ConclusionsNano-CT scans provide an efficient radiological tool in the planning of relevant experimental procedures. The present study confirms the general usability of fibre networks for the contactless creation of 3D strain fields in tissue engineering. Mechanical effects in tissue growth stimulation known from experimental work are obtained numerically for the investigated assemblies. Further work about the mechanical effects in tissue cultures appears highly worthwhile.Acknowledgements The authors wish to thank Dr Garth Wells for his advice about Timoshenko beam types, and boundary conditions. Also Dr Arul Britto is acknowledged for sharing his expertise about model implementation in Abaqus. Funding: This research was supported by the European Research Council GrantNo.240446 (http://erc.europa.eu/ )

Physical and biological characterization of ferromagnetic fiber networks: effect of fibrin deposition on short-term in vitro responses of human osteoblasts.

Ferromagnetic fiber networks have the potential to deform in vivo imparting therapeutic levels of strain on in-growing periprosthetic bone tissue. 444 Ferritic stainless steel provides a suitable material for this application due to its ability to support cultures of human osteoblasts (HObs) without eliciting undue inflammatory responses from monocytes in vitro. In the present article, a 444 fiber network, containing 17 vol% fibers, has been investigated. The network architecture was obtained by applying a skeletonization algorithm to three-dimensional tomographic reconstructions of the fiber networks. Elastic properties were measured using low-frequency vibration testing, providing globally averaged properties as opposed to mechanical methods that yield only local properties. The optimal region for transduction of strain to cells lies between the ferromagnetic fibers. However, cell attachment, at early time points, occurs primarily on fiber surfaces. Deposition of fibrin, a fibrous protein involved in acute inflammatory responses, can facilitate cell attachment within this optimal region at early time points. The current work compared physiological (3 and 5 g·L(-1)) and supraphysiological fibrinogen concentrations (10 g·L(-1)), using static in vitro seeding of HObs, to determine the effect of fibrin deposition on cell responses during the first week of cell culture. Early cell attachment within the interfiber spaces was observed in all fibrin-containing samples, supported by fibrin nanofibers. Fibrin deposition influenced the seeding, metabolic activity, and early stage differentiation of HObs cultured in the fibrin-containing fiber networks in a concentration-dependant manner. While initial cell attachment for networks with fibrin deposited from low physiological concentrations was similar to control samples without fibrin deposition, significantly higher HObs attached onto high physiological and supraphysiological concentrations. Despite higher cell numbers with supraphysiological concentrations, cell metabolic activities were similar for all fibrinogen concentrations. Further, cells cultured on supraphysiological concentrations exhibited lower cell differentiation as measured by alkaline phosphatase activity at early time points. Overall, the current study suggests that physiological fibrinogen concentrations would be more suitable than supraphysiological concentrations for supporting early cell activity in porous implant coatings.This is the author accepted manuscript. The final version is available from Mary Ann Liebert at http://online.liebertpub.com/doi/abs/10.1089/ten.TEA.2014.0211?url_ver=Z39.88-2003&rfr_id=ori%3Arid%3Acrossref.org&rfr_dat=cr_pub%3Dpubmed

Experimental and Numerical Design and Evaluation of a Vibration Bioreactor using Piezoelectric Patches

n this present study, we propose a method for exposing biological cells to mechanical vibration. The motive for our research was to design a bioreactor prototype in which in-depth in vitro studies about the influence of vibration on cells and their metabolism can be performed. The therapy of cancer or antibacterial measures are applications of interest. In addition, questions about the reaction of neurons to vibration are still largely unanswered. In our methodology, we used a piezoelectric patch (PZTp) for inducing mechanical vibration to the structure. To control the vibration amplitude, the structure could be excited at different frequency ranges, including resonance and non-resonance conditions. Experimental results show the vibration amplitudes expected for every frequency range tested, as well as the vibration pattern of those excitations. These are essential parameters to quantify the effect of vibration on cell behavior. Furthermore, a numerical model was validated with the experimental results presenting accurate results for the prediction of those parameters. With the calibrated numerical model, we will study in greater depth the effects of different vibration patterns for the abovementioned cell types.Postprint (published version

2018 Proceedings of the International Conference on Trauma Surgery Technology in Giessen

The overarching goal of the gathering was to define a concept for technology design research in Giessen to improve patient outcomes through the design of assistive technology that assists both patients and surgeons. The emerging field of regenerative rehabilitation where trauma patients are treated by methods of regenerative medicine promises great improvements for our field, but clinical success is yet to be realised. The symposium was specifically organised to acknowledge and address these issues

2019 Proceedings of the 2nd International Conference on Trauma Surgery Technology in Giessen (Germany)

It is now for a second time that we can invite researchers to come to Giessen for an international exchange of the latest research and a discussion of ideas. This year again, the Deutsche Forschungsgemeinschaft (DFG) is sponsoring the event. The main topic for 2019 is 'Vibration in antibacterial and oncological therapy'. Many effects of mechanical vibration on tissue have been discovered so far. Clinical applications relying on vibration exist for a variety of conditions. The intracellular processes, however, are still largely not understood. And reproducibility remains a matter of potential for improvement. DFG funds for the 3rd conference in 2020 have already been approved for a focus on multifunctional trauma surgery implants.Deutsche Forschungsgemeischaft (DFG), German