Enhancing Stability and Efficacy of Trichoderma Bio-Control Agents Through Layer-by-Layer Encapsulation for Sustainable Plant Protection

Agricultural fungicide pollution poses a significant environmental challenge and causes adverse effects on human health. Therefore, strategies to limit fungicide usage are of paramount importance. Trichoderma fungi, due to their antagonistic activity against various pathogenic fungi, have shown potential as a sustainable alternative to chemical fungicides. However, bio-control agents like Trichoderma are vulnerable to physical stimuli and show diminished efficacy during prolonged storage. To address these challenges, a mild and scalable encapsulation method for Trichoderma spores is introduced, employing a layer-by-layer (LbL) approach using biobased lignin derivatives. It is demonstrated that the LbL encapsulation technique significantly improved spore stability relative to naked spores, even under adverse conditions including extreme temperatures and prolonged exposure to Ultraviolet (UV) irradiation. Notably, encapsulated Trichoderma spores showed enhanced efficacy in cultivating tomato plants compared to naked spores. Additionally, the findings revealed that the in planta efficacy of encapsulated spores is dependent on the specific Trichoderma strain used. This study suggests that Trichoderma spores encapsulated with lignin through the LbL approach, are a promising and sustainable alternative to chemical fungicides with potential for commercialization.</p

Enhancing Stability and Efficacy of Trichoderma Bio-Control Agents through Layer-by-Layer Encapsulation for Sustainable Plant Protection

Agricultural fungicide pollution poses a significant environmental challenge and carries adverse consequences for human health. Therefore, strategies to limit fungicide usage have gained paramount importance. Trichoderma fungi, owing to their antagonistic activity against various pathogenic fungi, have emerged as prospective candidates for enhancing both the effectiveness and sustainability of plant protection. Nevertheless, the utilization of bio-control agents like Trichoderma has unveiled new challenges, notably their vulnerability to physical stimuli and diminished efficacy during prolonged storage. To overcome these drawbacks, we present a mild and scalable encapsulation method for Trichoderma spores, employing a layer-by-layer (LbL) encapsulation approach using biobased lignin derivates. Our investigations demonstrate that the LbL-encapsulation technique imparts remarkable improvements in spore stability, even under adverse conditions such as variable temperature and prolonged exposure to UV irradiation compared to unencapsulated spores. Notably, encapsulated Trichoderma spores exhibit increased efficiency in the cultivation of tomato plants when compared to their unencapsulated counterparts. Additionally, our findings reveal that the in planta efficacy of encapsulated spores is contingent upon the specific Trichoderma strain employed. The results outlined herein suggest that Trichoderma spores, encapsulated within lignin through the LbL approach, exhibit potential as promising and sustainable alternative to chemical fungicides and potential commercialization

Mere undervisning, større studieintensitet? En multilevelanalyse af 7.917 studerendes tidsforbrug

Der har de seneste år været betydelig interesse for danske universitetsstuderendes studieintensitet, og der er blevet givet forskellige anbefalinger i forhold til, hvordan de studerende kan motiveres til at studere mere. Det empiriske grundlag for sådanne anbefalinger er imidlertid sparsomt. Formålet med dette studie er dels at beskrive den gennemsnitlige studieaktivitet blandt studerende på Aarhus Universitet, dels at analysere det indbyrdes forhold mellem undervisning og forberedelse. En multilevelanalyse af 7.917 studerendes selvrapporterede tidsforbrug viser overraskende en negativ sammenhæng mellem antallet af timer brugt på undervisning og antallet af timer brugt på forberedelse. Yderligere analyser tyder på, at sammenhængen mellem undervisningstid og forberedelsestid varierer på tværs af uddannelser. In recent years, the study habits of Danish university students have been a cause for concern, and various initiatives to improve motivation and encourage students to study harder have been introduced. However, the empirical support for such initiatives is limited. This article aims to describe the average student’s study habits and analyse the interrelationship between teaching and independent study time. A multilevel analysis of 7,917 students self-reported time budgets surprisingly reveals negative correlation between hours spent studying and hours spent participating in classes. Further analyses suggest that this correlation varies across study programmes.

The Apolipoprotein M/S1P Axis Controls Triglyceride Metabolism and Brown Fat Activity

Summary: Apolipoprotein M (apoM) is the carrier of sphingosine-1-phosphate (S1P) in plasma high-density lipoproteins. S1P is a bioactive lipid interacting with five receptors (S1P1–5). We show that lack of apoM in mice increases the amount of brown adipose tissue (BAT), accelerates the clearance of postprandial triglycerides, and protects against diet-induced obesity (i.e., a phenotype similar to that induced by cold exposure or β3-adrenergic stimulation). Moreover, the data suggest that the phenotype of apoM-deficient mice is S1P dependent and reflects diminished S1P1 stimulation. The results reveal a link between the apoM/S1P axis and energy metabolism. : Apolipoprotein M (apoM) is the carrier of sphingosine-1-phosphate (S1P) in lipoproteins. Christoffersen et al. show that lack of apoM in mice increases the amount of brown adipose tissue, accelerates the turnover of fat, and protects against obesity. The results reveal a link between the apoM/S1P axis and energy metabolism. Keywords: apolipoproteins, sphingolipids, sphingosine-1-phosphate, lipoproteins, lipid metabolism, triglyceride, brown adipose tissue, apo

Severity of cardiovascular disease outcomes among patients with HIV is related to markers of inflammation and coagulation

Background-In the general population, raised levels of inflammatory markers are stronger predictors of fatal than nonfatal cardiovascular disease (CVD) events. People with HIV have elevated levels of interleukin-6 (IL-6), high-sensitivity C-reactive protein (hsCRP), and D-dimer; HIV-induced activation of inflammatory and coagulation pathways may be responsible for their greater risk of CVD. Whether the enhanced inflammation and coagulation associated with HIV is associated with more fatal CVD events has not been investigated. Methods and Results-Biomarkers were measured at baseline for 9764 patients with HIV and no history of CVD. Of these patients, we focus on the 288 that experienced either a fatal (n=74) or nonfatal (n=214) CVD event over a median of 5 years. Odds ratios (ORs) (fatal versus nonfatal CVD) (95% confidence intervals [CIs]) associated with a doubling of IL-6, D-dimer, hsCRP, and a 1-unit increase in an IL-6 and D-dimer score, measured a median of 2.6 years before the event, were 1.39 (1.07 to 1.79), 1.40 (1.10 to 1.78), 1.09 (0.93 to 1.28), and 1.51 (1.15 to 1.97), respectively. Of the 214 patients with nonfatal CVD, 23 died during follow-up. Hazard ratios (95% CI) for all-cause mortality were 1.72 (1.28 to 2.31), 1.73 (1.27 to 2.36), 1.44 (1.15 to 1.80), and 1.88 (1.39 to 2.55), respectively, for IL-6, D-dimer, hsCRP, and the IL-6 and D-dimer score. Conclusions-Higher IL-6 and D-dimer levels reflecting enhanced inflammation and coagulation associated with HIV are associated with a greater risk of fatal CVD and a greater risk of death after a nonfatal CVD even

Aging mechanism as the "down side" of adaptation:a network approach

International audienc