Polyserositis (Concato's disease) due to granulocyte colony stimulating factor therapy for lymphoma

Polyserositis is defined as general inflammation of serous membranes associated with serous effusion due to many causes. In this study, we evaluated polyserositis as a rare complication of GCSF therapy. A 26-year-old man with a history of lymphoma was referred to our hospital complaining of dyspnea, tachycardia, tachypnea, chest pain, bilateral pleural effusion, ascites and massive pericardial effusion after 5 days of treatment with GCSF. After 7 days of treatment with indomethacin, dexamethasone and discontinuation of GCSF, the patient improved and was referred to an oncologist for chemotherapy of lymphoma. Based on this case report, we should consider various causes of pleural effusion in a patient with lymphoma, and use drugs with caution in such patients as they may be predisposed to dyspnea and fluid retention. ©2009 NRITLD, National Research Institute of Tuberculosis and Lung Disease

Detection and identification of Leishmania isolates from patients with Cutaneous Leishmaniasis (CL) in Isfahan (central region of Iran) by PCR method

Leishmaniasis is caused by parasitic protozoa of the genus Leishmania. Cutaneous leishmaniasis (CL) is a complex disease with wide spectrum of clinical manifestations. In order to identify leishmania species causing CL in Isfahan by a definite molecular technique (PCR method), this study was undertaken over 2010- 2011. 124 Patients with suspicious lesion of Leishmaniasis and positive direct smear from lesion were selected. Samples were cultured in NNN and RPMI 1640 media Negative and positive control and clinical samples was applied for PCR in the same condition. In the next step, standard PCR was carried out using classic protocol. From 124 patients, 111 (89.51%) cases were infected as L. major and 12 (9.67%) cases were infected by L. tropica, However only in one patient simultaneous infectious with both L. major and L. tropica was identified by PCR techniques which could not be possible in microscopy. L.major was the most prevalent species in the studied patients (p-value<0.001)